Toxicological assessment of organohalogen contaminants in cat (Felis catus) omics approach
Project/Area Number |
16H02989
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Environmental risk control and evaluation
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Research Institution | Ehime University |
Principal Investigator |
Nomiyama Kei 愛媛大学, 沿岸環境科学研究センター, 准教授 (30512686)
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Co-Investigator(Kenkyū-buntansha) |
水川 葉月 北海道大学, 獣医学研究院, 博士研究員 (60612661)
国末 達也 愛媛大学, 沿岸環境科学研究センター, 教授 (90380287)
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Research Collaborator |
Ishizuka Mayumi
Iwata Hisato
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Project Period (FY) |
2016-04-01 – 2019-03-31
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Project Status |
Completed (Fiscal Year 2018)
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Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2018: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2017: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2016: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
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Keywords | ネコ / PCBs / OH-PCBs / トランスクリプトーム / メタボローム / in vivo / PBDEs / イエネコ / 腎集積 / 免疫異常 / 甲状腺ホルモン / オミックス / イヌ / 脳移行 / プロテオーム / 酸化的リン酸化 / リスク評価 / トキシコロジー / 代謝 / メタボロミクス / 酸化ストレス |
Outline of Final Research Achievements |
In vivo exposure of 12 PCB congeners mixture were conducted on cats, and analyzed transcriptome and metabolome in blood, liver and brain. Of the 21890 genes in the transcriptome, 531 genes showed significant differences in the PCBs-exposed group. As a result of the pathway analysis, the inflammation and infection related pathways are affected, and the influence on the immune system by PCBs was suggested. In metabolome analysis of serum, 122 metabolites showed significant difference. As a result of pathway analysis, metabolome related to central carbon metabolism such aspentose phosphate pathway and histidine metabolic system decreased, suggesting suppression of these metabolic pathways accompanying PCBs exposure.
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Academic Significance and Societal Importance of the Research Achievements |
本研究によりネコが有機ハロゲン化合物に高濃度曝露され、免疫系や甲状腺系、酸化ストレスの増大等の多様な悪影響を受けていることが示唆された。ネコのin vivo試験を実施した研究は極めて少なく、ペット動物の汚染実態と生理学的健康リスクの評価といった包括的なアプローチを試みた研究は世界的にみても例がなく、本申請研究成果は先導的である。 ネコは身近なペット動物であるにも関わらず、化学物質による汚染の実態と生体影響を調査した事例は乏しい。ヒトと生活環境を共にすることで、ネコは多種多様な化学物質に曝露されていることが予想される。本研究で化学物質の曝露と影響の一端が示され、学術的・社会的波及効果は大きい。
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Report
(4 results)
Research Products
(60 results)
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[Journal Article] Polybrominated diphenyl ethers (PBDEs) and their hydroxylated and methoxylated analogues in the blood of harbor, Dall's and finless porpoises from the Japanes e coastal waters2016
Author(s)
Ochiai, M., Nomiyama, K., Isobe, T., Yamada, T. K., Tajima, Y., Matsuda, A., Shiozaki, A., Matsuishi, T., Amano, M., Iwata, H., Tanabe, S.
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Journal Title
Marine Environmental Research
Volume: 1136(16)
Pages: 30260-30264
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] Effects of polychlorinated biphenyls (PCBs) in cats: Exhaustive gene expression analysis approach2018
Author(s)
Mizukawa, H., Khidkhan, K., Ikenaka, Y., Nakayama, S. M. M., Nomiyama, K., Yokoyama, N., Ichii, O., Takiguchi, M., Tsend-ayush, S., Tanabe, S and Ishizuka
Organizer
38th International Symposium on Halogenated Persistent Organic Pollutants (DIOXIN 2018)
Related Report
Int'l Joint Research
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[Presentation] Contamination status and global comparison of organohalogen compounds and their related compounds in the pet cats in Thailand2018
Author(s)
Shimasaki, M., Nomiyama, K., Mizukawa, H., Saengtienchai, A., Ngamchirttakul, A., Pencharee, D., Ikenaka, Y., Nakayama, S., Kunisue, T. and Tanabe, S
Organizer
38th International Symposium on Halogenated Persistent Organic Pollutants (DIOXIN 2018)
Related Report
Int'l Joint Research
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[Presentation] Tissue distribution and CYP expression related-PCBs exposure in cats.2018
Author(s)
Khidkhan, K., Mizukawa, H., Ikenaka, T., Nakayama, S., Darwish, W.S., Nomiyama, K., Takiguchi, M., Yokoyama, N., Ichii, O., Tanabe, S. and Ishizuka, M
Organizer
38th International Symposium on Halogenated Persistent Organic Pollutants (DIOXIN 2018)
Related Report
Int'l Joint Research
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[Presentation] Pilot study on the toxicological assessment of organohalogen compounds in pet cat (Felis catus) serum using metabolomics approach2018
Author(s)
Nomiyama, K., Eguchi, A., Mizukawa, H., Yamamoto, Y., Nishikawa, H., Takiguchi, M., Nakayama, S., Ikenaka, Y., Isizuka, M. and Tanabe, S.
Organizer
38th International Symposium on Halogenated Persistent Organic Pollutants (DIOXIN 2018)
Related Report
Int'l Joint Research
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[Presentation] PCBs as an environmental obesogen in dogs: evidence from hepatic transcriptome, metabolome, and lipidome analyses.2018
Author(s)
Tamura, S., Agusa, T., Hirano, M., Eguchi, A., Nomiyama, K., Li, L., Kannan, K., Tanabe, S., Kim, E. Y. and Iwata, H.
Organizer
38th International Symposium on Halogenated Persistent Organic Pollutants (DIOXIN 2018)
Related Report
Int'l Joint Research
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[Presentation] In vitro and in silico approaches for assessing the activation of Baikal seal estrogen receptors by bisphenols and OH-PCBs2018
Author(s)
Yoshinouchi, Y., Hirano, M., Nakata, H., Nomiyama, K., Tanabe, S., Kim, E. Y. and Iwata, H.
Organizer
38th International Symposium on Halogenated Persistent Organic Pollutants (DIOXIN 2018)
Related Report
Int'l Joint Research
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[Presentation] Effects of PCB exposure on thyroid hormone levels in serum of dogs and cats2018
Author(s)
Takaguchi, K., Nishikawa, H., Mizukawa, H., Tanoue, R., Yokoyama, N., Ichii, O., Takiguchi, M., Nakayama, S. M., Ikenaka, Y., Kunisue, T., Ishizuka, M., Tanabe, S., Iwata, H. and Nomiyama, K.
Organizer
SETAC North America 39th Annual Meeting
Related Report
Int'l Joint Research
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[Presentation] UDP-glucuronosyltransferase (UGT) among pinnipedia and other carnivores.2017
Author(s)
Kondo, T., Ikenaka, Y., Nakayama, S. M. M., Kawai, Y. K., Mizukawa, H., Mitani, Y., Nomiyama, K., Tanabe, S. and Ishizuka, M.
Organizer
19th International Symposium on Pollutant Responses in Marine Organisms (PRIMO19)
Related Report
Int'l Joint Research
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[Presentation] Identification for the biotransformation of BDE209 by in vivo toxicokinetic analysis in Felis catus.2017
Author(s)
Tanaka, K., Nomiyama, K., Mizukawa, H., Takaguchi, K., Tanoue, R., Saengtienchai, A., Sainnoxoi, T., Yokoyama, N., Ichii, O., Takiguchi, M., Nakayama, S. Ikenaka, Y., Ishizuka, M., Iwata, H., Kunisue, T. and Tanabe, S.
Organizer
19th International Symposium on Pollutant Responses in Marine Organisms (PRIMO19)
Related Report
Int'l Joint Research
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[Presentation] Effects of PCB-exposure on thyroid hormone homeostasis of dogs and cats.2017
Author(s)
Takaguchi, K., Nomiyama, K., Nishikawa, H., Mizukawa, H., Tanoue, R., Yoshinouchi, Y., Yokoyama, N., Ichii, O., Takiguchi, M., Nakayama, S., Ikenaka, Y., Ishizuka, M., Iwata, H., Kunisue, T. and Tanabe, S.
Organizer
19th International Symposium on Pollutant Responses in Marine Organisms (PRIMO19)
Related Report
Int'l Joint Research
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[Presentation] In vivo exposure to PCBs in cats: Analysis of metabolic capacities and effects on the thyroid hormone homeostasis.2017
Author(s)
Mizukawa, H., Nomiyama, K., Nishikawa, H., Maehara, M., Takai, N., Yokoyama, N., Ichii, O., Takiguchi, M., Ikenaka, Y., Shouta, M. M., Takaguchi, K., Tanabe, S. and Ishizuka, M.
Organizer
37th International Symposium on Halogenated Persistent Organic Pollutants (DIOXIN 2017)
Related Report
Int'l Joint Research
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[Presentation] Tissue distribution of polychlorinated biphenyls and their metabolites in dog and cat.2017
Author(s)
Takaguchi, K., Nomiyama, K., Nagano, Y., Mizukawa, H., Nakatsu, S., Kunisue, T. and Tanabe, S.
Organizer
37th International Symposium on Halogenated Persistent Organic Pollutants (DIOXIN 2017)
Related Report
Int'l Joint Research
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[Presentation] Identification for the biotransformation of BDE209 by in vivo toxicokinetic analysis in Felis catus.2017
Author(s)
Tanaka, K., Nomiyama, K., Mizukawa, H., Takaguchi, K., Tanoue, R., Saengtienchai, A., Sainnoxoi, T., Yokoyama, N., Ichii, O., Takiguchi, M., Nakayama, S., Ikenaka, Y., Ishizuka, M., Kunisue, T. and Tanabe, S.
Organizer
37th International Symposium on Halogenated Persistent Organic Pollutants (DIOXIN 2017)
Related Report
Int'l Joint Research
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[Presentation] DecaBDE might be associated with inhibition of thyroid hormone levels in serum of Japanese cats.2017
Author(s)
Nomiyama, K., Yamamoto, Y., Mizukawa, H., Takiguchi, M., Eguchi, A., Nakayama, S., Ikenaka, Y., Ishizuka, M., Kunisue, T. and Tanabe, S.
Organizer
37th International Symposium on Halogenated Persistent Organic Pollutants (DIOXIN 2017)
Related Report
Int'l Joint Research
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[Presentation] Serum biochemical responses in cats exposed to organohalogen compounds.2017
Author(s)
Kraisiri, K., Mizukawa, H., Ikenaka, Y., Nakayama, S. M. M., Nomiyama, K., Yokoyama, N., Sasaoka, K., Ichii, O., Takiguchi, M., Nishikawa, H., Tanaka, K., Takaguchi, K., Tanabe, S. and Ishizuka, M.
Organizer
Chemical Hazard Symposium (Joint Seminar on Environmental Chemistry and Toxicology)
Related Report
Int'l Joint Research
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[Presentation] Accumulation features of organohalogen compounds and their hydroxylated metabolites in pet cats and dogs: effects on thyroid hormones homeostasis.2016
Author(s)
Nomiyama, K., Yamamoto, Y., Mizukawa, H., Eguchi, A., Nakayama, S. M., Ikenaka, Y., Ishizuka, M., Kunisue, T. and Tanabe, S.
Organizer
SETAC North America 37th Annual Meeting/7th SETAC World Congress
Place of Presentation
Orlando, FL, USA
Year and Date
2016-11-06
Related Report
Int'l Joint Research
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[Presentation] Characteristic for biotransformation of polychrolinated biphenyls in cats.2016
Author(s)
Mizukawa, H., Maehara, M., Yokoyama, N., Ichii, O., Takiguchi, M., Nomiyama, K., Nishikawa, H., Ikenaka, Y., Nakayama, S. M., Takaguchi, K., Tanabe, S., Ishizuka, M.
Organizer
SETAC North America 37th Annual Meeting/7th SETAC World Congress
Place of Presentation
Orlando, FL, USA
Year and Date
2016-11-06
Related Report
Int'l Joint Research
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[Presentation] Cross omics analysis for PCBs toxicity in the dog brain - Effect on mitochondrial functions-2016
Author(s)
Takaguchi, K., Nomiyama, K., Yoo, J., Mizukawa, H., Iwata, H., Kunisue, T. and Tanabe, S.
Organizer
SETAC North America 37th Annual Meeting/7th SETAC World Congress
Place of Presentation
Orlando, FL, USA
Year and Date
2016-11-06
Related Report
Int'l Joint Research
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[Presentation] In vitro and in silico assessment of cytochrome P450-dependent biotransformation capacity of PCBs in the Caniformia; the Baikal seal vs the beagle dog2016
Author(s)
Iwata, H., Yoo, J., Tamura, S., Hirano, M., Nomiyama, K. and Tanabe, S.
Organizer
SETAC North America 37th Annual Meeting/7th SETAC World Congress
Place of Presentation
Orlando, FL, USA
Year and Date
2016-11-06
Related Report
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[Presentation] Toxicological assessment of PCBs and OH-PCBs in the brain of dogs using metabolic approach.2016
Author(s)
Nomiyama, K., Takaguchi, K., Eguchi, A., Mizukawa, H., Yoo, J., Tamaru, S., Oshihoi, T., Iwata, H., Kunisue, T. and Tanabe, S.
Organizer
The 9th International PCB Workshop
Place of Presentation
神戸国際会議場, 兵庫, 神戸市
Year and Date
2016-10-09
Related Report
Int'l Joint Research / Invited
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[Presentation] In vivo analysis of PCB metabolic capacities and effects on the thyroid hormone in cats.2016
Author(s)
Mizukawa, H., Maehara, M., Yokoyama, N., Ichii, O., Takiguchi, M., Nomiyama, K., Nishikawa, H., Takakuchi, K., Saengtienchai, A., Sainnoxoi, T., Ikenaka, Y., Nakayama, S. M. M., Tanabe, S. and Ishizuka, M.
Organizer
The 9th International PCB Workshop
Place of Presentation
神戸国際会議場, 兵庫, 神戸市
Year and Date
2016-10-09
Related Report
Int'l Joint Research
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[Presentation] Disruption of mitochondrial functions by PCB exposure in the dog brain: Toxicological assessment using cross omics.2016
Author(s)
Takaguchi, K., Nomiyama, K., Eguchi, A., Claret, L. M., Kusaki, M., Yoo, J., Mizukawa, H., Iwata, H., Kunisue, T. and Tanabe, S.
Organizer
The 9th International PCB Workshop
Place of Presentation
神戸国際会議場, 兵庫, 神戸市
Year and Date
2016-10-09
Related Report
Int'l Joint Research
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[Presentation] Hepatic transcriptome analysis to assess the effects of polychlorinated biphenyls on beagle dogs2016
Author(s)
Tamura, S., Agusa, T., Hirano, M., Eguchi, A., Nomiyama, K., Tanabe, S., Kim, E. and Iwata, H.
Organizer
The 9th International PCB Workshop
Place of Presentation
神戸国際会議場, 兵庫, 神戸市
Year and Date
2016-10-09
Related Report
Int'l Joint Research
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[Presentation] Contamination status of organohalogen compounds and their hydroxylated metabolites in pet cats and dogs: effects on thyroid hormones homeostasis.2016
Author(s)
Nomiyama, K., Yamamoto, Y., Mizukawa, H., Takaguchi, K., Takiguchi, M., Eguchi, A., Nakayama, S., Ikenaka, Y., Ishizuka, M., Kunisue, T. and Tanabe, S.
Organizer
36th International Symposium on Halogenated Persistent Organic Pollutants (DIOXIN 2016)
Place of Presentation
Firenze, Italy
Year and Date
2016-08-28
Related Report
Int'l Joint Research
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