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Development of a device for capturing immune cells

Research Project

Project/Area Number 16H03181
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biomedical engineering/Biomaterial science and engineering
Research InstitutionTokyo Medical and Dental University

Principal Investigator

KIMURA Tsuyoshi  東京医科歯科大学, 生体材料工学研究所, 准教授 (10393216)

Co-Investigator(Kenkyū-buntansha) 中村 奈緒子  芝浦工業大学, システム理工学部, 助教 (70754878)
Research Collaborator HASHIMOTO Yoshihide  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2018: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2017: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2016: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Keywords生体材料 / バイオ界面 / 免疫学
Outline of Final Research Achievements

Regulatory T cells (Tregs) are important role for cancer immunotherapy and autoimmune disease because Tregs show immune suppressive functions. For cancer immunotherapy, Tregs prevent effector T cells attacking to cancer cells. For autoimmune disease, when Tregs doesn’t work well, effector T cells attack themselves. So, in the preset study, we developed a device that capture Tregs and effector T cell specifically in order to regulate the balance of them in body. We designed the device loading a filter that was modified with non-cell adhesive polymer grafting and antibodies against Treg and effector T cells in order to capture them selectively. The filter could capture target cells effectively and selectively. The captured cells maintained the cell functions. Also, the target cells were captured under flow condition. These results suggest that the device capturing target cells was developed.

Academic Significance and Societal Importance of the Research Achievements

本研究で開発した免疫細胞捕集デバイスは、循環中の標的とする免疫細胞を選択的に捕獲することができ、捕獲した細胞の機能を維持させつつ回収することが可能であり、免疫調節における材料学的な点から新しい方法論と考えられる。また、本研究は免疫細胞のみならず多くの細胞種に対しても応用可能であり発展性のある研究と位置づけられる。さらに、体内での免疫調節法としての新たな知見も見出され、材料学の発展に寄与すると考えられる。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (25 results)

All 2019 2018 2017 2016 Other

All Journal Article (6 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 6 results,  Acknowledgement Compliant: 1 results) Presentation (17 results) (of which Int'l Joint Research: 4 results) Book (1 results) Remarks (1 results)

  • [Journal Article] A hybrid small‐diameter tube fabricated from decellularized aortic intima‐media and electrospun fiber for artificial small‐diameter blood vessel2019

    • Author(s)
      Wu Pingli、Nakamura Naoko、Morita Hiroko、Nam Kwangwoo、Fujisato Toshiya、Kimura Tsuyoshi、Kishida Akio
    • Journal Title

      Journal of Biomedical Materials Research Part A

      Volume: 107 Issue: 5 Pages: 1064-1070

    • DOI

      10.1002/jbm.a.36631

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Surface Topography of PDMS Replica Transferred from Various Decellularized Aortic Lumens Affects Cellular Orientation2019

    • Author(s)
      Kimura Tsuyoshi、Kondo Mayuka、Hashimoto Yoshihide、Fujisato Toshiya、Nakamura Naoko、Kishida Akio
    • Journal Title

      ACS Biomaterials Science & Engineering

      Volume: 印刷中 Issue: 11 Pages: 5721-5726

    • DOI

      10.1021/acsbiomaterials.8b01536

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Induction of in Vivo Ectopic Hematopoiesis by a Three-Dimensional Structured Extracellular Matrix Derived from Decellularized Cancellous Bone2019

    • Author(s)
      Nakamura Naoko、Kimura Tsuyoshi、Nam Kwangwoo、Fujisato Toshiya、Iwata Hiroo、Tsuji Takashi、Kishida Akio
    • Journal Title

      ACS Biomaterials Science & Engineering

      Volume: 印刷中 Issue: 11 Pages: 5669-5680

    • DOI

      10.1021/acsbiomaterials.8b01491

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Selective Cell Capture and Release Using Antibody-Immobilized Polymer-Grafted Surface2018

    • Author(s)
      木村剛、中村奈緒子、橋本良秀、坂口志文、木村俊作、岸田晶夫、
    • Journal Title

      KOBUNSHI RONBUNSHU

      Volume: 75 Issue: 2 Pages: 155-163

    • DOI

      10.1295/koron.2017-0074

    • NAID

      130006528515

    • ISSN
      0386-2186, 1881-5685
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Capture and release of cells using a temperature-responsive surface that immobilizes an antibody through DNA duplex formation2017

    • Author(s)
      Kimura Tsuyoshi、Nakamura Naoko、Umeda Kanji、Hashimoto Yoshihide、Kishida Akio
    • Journal Title

      Journal of Biomaterials Science: Polymer Edition

      Volume: 28 Issue: 10-12 Pages: 1172-1182

    • DOI

      10.1080/09205063.2017.1309124

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Capture and Release of Target Cells Using a Surface that Immobilizes an Antibody via Desthiobiotin–Avidin Interaction2016

    • Author(s)
      Tsuyoshi Kimura, Naoko Nakamura, Nao Sasaki, Yoshihide Hashimoto, Shimon Sakaguchi, Shunsaku Kimura, and Akio Kishida
    • Journal Title

      Sensors and Materials

      Volume: 28 Pages: 1

    • DOI

      10.18494/SAM.2016.1287

    • ISSN
      0914-4935, 2435-0869
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Development of an antibody immobilizing device for label-free selective capture of target cells2018

    • Author(s)
      Tsuyoshi Kimura, Naoko Nakamura, Shimon Sakaguchi, Shunsaku Kimura, Akio Kishida
    • Organizer
      Finland-Japan Workshop: The next generation medical engineering in biomaterials
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 抗体固定化フィルターによる免疫細胞捕集2018

    • Author(s)
      木村剛, 中村奈緒子, 橋本良秀、坂口志文, 木村俊作, 岸田晶夫
    • Organizer
      第67回高分子討論会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Development of antibody-immobilized surface for capture of target cells2018

    • Author(s)
      Naoko Nakamura, Takumi Akaiwa, Tsuyoshi Kimura, Akio Kishida
    • Organizer
      International Symposium on Biomedical Engineering 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 生体内免疫調節を目指した細胞捕集技術開発2018

    • Author(s)
      木村剛、橋本良秀、中村奈緒子、岸田晶夫
    • Organizer
      第27回ライフサポート学会フロンティア講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 制御性T細胞の選択的捕獲と機能評価2017

    • Author(s)
      木村剛, 中村奈緒子, 巻口遥翔, 橋本良秀, 坂口志文, 木村俊作, 岸田晶夫
    • Organizer
      第26回インテリジェント材料・システムシンポジウム
    • Place of Presentation
      東京女子医科大学(東京都新宿区)
    • Year and Date
      2017-01-11
    • Related Report
      2016 Annual Research Report
  • [Presentation] Selective capture of regulatory T cells for cancer immunotherapy2017

    • Author(s)
      Tsuyoshi Kimura, Naoko Nakamura, Haruto Makiguchi, Yoshihide Hashimoto, Shimon Sakaguchi, Shunsaku Kimura and Akio Kishida
    • Organizer
      Society For Biomaterials 2017 Annual Meeting & Exposition
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Label-free capture of lymphocytes using a surface immobilizing antibody2017

    • Author(s)
      Tsuyoshi Kimura, Naoko Nakamura, Yoshihide Hashimoto, Shimon Sakaguchi, Shunsaku Kimura and Akio Kishida
    • Organizer
      第66回高分子学会年次大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 免疫系セルセパレーターの開発を目指した界面設計2017

    • Author(s)
      木村剛, 中村奈緒子, 橋本良秀、坂口志文, 木村俊作, 岸田晶夫
    • Organizer
      第46回医用高分子シンポジウム
    • Related Report
      2017 Annual Research Report
  • [Presentation] 抗体固定化フィルターを用いた免疫細胞捕集2017

    • Author(s)
      木村剛, 中村奈緒子, 橋本良秀、坂口志文, 木村俊作, 岸田晶夫
    • Organizer
      第66回高分子討論会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 免疫細胞捕集フィルターの開発2017

    • Author(s)
      木村剛, 中村奈緒子, 橋本良秀、坂口志文, 木村俊作, 岸田晶夫
    • Organizer
      第39回日本バイオマテリアル学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Development Of An Antibody Immobilizing Device For Label-Free Selective Capture And Collection Of Stem Cells2017

    • Author(s)
      Tsuyoshi Kimura, Naoko Nakamura, Yoshihide Hashimoto and Akio Kishida
    • Organizer
      TERMIS-Americas
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 抗体固定化基材を用いた細胞の捕獲と回収2016

    • Author(s)
      木村剛, 中村奈緒子, 坂口志文, 木村俊作, 岸田晶夫
    • Organizer
      2016 MRS年次大会
    • Place of Presentation
      横浜市開港記念会館(神奈川県横浜市)
    • Year and Date
      2016-12-19
    • Related Report
      2016 Annual Research Report
  • [Presentation] Label-free capture and collection of target cells using a surface immobilizing antibody2016

    • Author(s)
      Tsuyoshi Kimura, Naoko Nakamura, Yoshihide Hashimoto, Shimon Sakaguchi, Shunsaku Kimura and Akio Kishida
    • Organizer
      TERMIS-AM(国際学会)
    • Place of Presentation
      San Diego, USA
    • Year and Date
      2016-12-11
    • Related Report
      2016 Annual Research Report
  • [Presentation] Capture and release of lymphocyte on an antibody-immobilized surface2016

    • Author(s)
      Tsuyoshi Kimura, Naoko Nakamura, Yoshihide Hashimoto, Shimon Sakaguchi, Shunsaku Kimura, and Akio Kishida
    • Organizer
      3rd international Conference on Biomaterials Science in Tokyo(国際学会)
    • Place of Presentation
      東京大学伊藤ホール(東京都文京区)
    • Year and Date
      2016-11-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] 抗体修飾デバイスによる細胞の選択的捕集と細胞機能評価2016

    • Author(s)
      木村剛, 中村奈緒子, 巻口遥翔, 橋本良秀、坂口志文, 木村俊作, 岸田晶夫
    • Organizer
      バイオマテリアルシンポジウム2016
    • Place of Presentation
      九州国際会議場(福岡県福岡市)
    • Year and Date
      2016-11-21
    • Related Report
      2016 Annual Research Report
  • [Presentation] 免疫系細胞特異的な捕獲・回収デバイスの開発2016

    • Author(s)
      木村剛, 中村奈緒子, 佐々木直, 坂口志文, 木村俊作, 岸田晶夫
    • Organizer
      第65回高分子討論会
    • Place of Presentation
      神奈川大学(神奈川県横浜市)
    • Year and Date
      2016-09-15
    • Related Report
      2016 Annual Research Report
  • [Presentation] 抗体修飾基材を用いた細胞の特異的捕獲と回収2016

    • Author(s)
      木村剛, 中村奈緒子, 佐々木直, 坂口志文, 木村俊作, 岸田晶夫
    • Organizer
      第65回高分子学会年次大会
    • Place of Presentation
      神戸国際展示場(兵庫県神戸市)
    • Year and Date
      2016-05-25
    • Related Report
      2016 Annual Research Report
  • [Book] 歯科再生医学2019

    • Author(s)
      木村剛、岸田晶夫
    • Total Pages
      321
    • Publisher
      医歯薬出版
    • ISBN
      4263458389
    • Related Report
      2018 Annual Research Report
  • [Remarks] 東京医科歯科大学生体材料工学研究所物質医工学分野ホームページ

    • URL

      http://www.labonet.info/kishida/

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report 2016 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2020-03-30  

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