Therapeutic strategy for diabetic microcirculatory disorder using transcutaneous carbon dioxide absorption combined pulsed ultrasonic irradiation
| Project/Area Number |
16H03208
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Rehabilitation science/Welfare engineering
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| Research Institution | Nagoya Women's University |
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Principal Investigator |
Kondo Hiroyo 名古屋女子大学, 健康科学部, 准教授 (50333183)
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| Co-Investigator(Kenkyū-buntansha) |
藤野 英己 神戸大学, 保健学研究科, 教授 (20278998)
石原 昭彦 京都大学, 人間・環境学研究科, 教授 (90184548)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2018: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
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| Keywords | 微小循環 / 代謝 / 糖尿病 / 二酸化炭素 / 血流 / 毛細血管 / 血管 / 物理療法 |
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| Outline of Final Research Achievements |
Chronic hyperglycemia exposure caused a decrease in citrate synthase activity and regression of capillary network in skeletal muscle. Carbon absorption suppressed the decline of citrate synthase activity and capillary regression due to hyperglycemia and suppressed blood glucose rise. Furthermore, the expression levels of eNOS, PGC-1α, COX IV, and VEGF proteins related to metabolism and angiogenesis were increased and the expression level of angiogenesis inhibitor (TSP-1) was decreased in skeletal muscle. Pulsed ultrasound irradiation increased phosphorylation of integrin/focal adhesion kinase (FAK) and decrease phosphorylation of p38MAPK in myotubes induced inflammation. The irradiation also reduced the expression of inflammatory cytokines. These results indicated that percutaneous absorption of carbon dioxide and pulsed ultrasound therapy could be an effective intervention against microangiopathy induced by diabetes.
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| Academic Significance and Societal Importance of the Research Achievements |
これらの研究結果から経皮的二酸化炭素吸収は，微小血管におけるずり応力とeNOS発現を増加させ，骨格筋の代謝や血管新生因子の増加と血管新生抑制因子の低下を引き起こし，高血糖曝露による骨格筋の酸化的リン酸化の機能低下や毛細血管退行を抑制することを明らかにした．また，パルス超音波照射はp38MAPKのリン酸化を抑制することで炎症を軽減させることが観察された．本研究成果から経皮的炭酸ガス吸収とパルス超音波による糖尿病性微小循環障害の予防改善への可能性が示された．
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Report
(4 results)
Research Products
(114 results)
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[Journal Article] Transcutaneous carbon dioxide attenuates impaired oxidative capacity in skeletal muscle in hyperglycemia2019
Author(s)
Matsumoto T, Tanaka M, Nakanishi R, Takuwa M, Hirabayashi T, Ono K, Ikeji T, Maeshige N, Sakai Y, Akisue T, Kondo H, Ishihara A, Fujino H
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Journal Title
General Physiology and Biophysics
Volume: -
Related Report
Peer Reviewed
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[Journal Article] Preventive effects of low-intensity exercise on cancer cachexia-induced muscle atrophy2019
Author(s)
Tanaka M, Sugimoto K, Fujimoto T, Xie K, Takahashi T, Akasaka H, Kurinami H, Yasunobe Y, Matsumoto T, Fujino H, Rakugi H
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Journal Title
FASEB J
Volume: 27
Issue: 7
Pages: 7852-7862
DOI
Related Report
Peer Reviewed
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[Journal Article] Inhibitory Effects of Short-Chain Fatty Acids and ω-3 Polyunsaturated Fatty Acids on Profibrotic Factors in Dermal Fibroblasts2019
Author(s)
Maeshige N, Torii K, Tabuchi H, Imai M, Koga Y, Uemura M, Aoyama-Ishikawa M, Miyoshi M, Fujino H, Terashi H, Usami M
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Journal Title
NAID
Related Report
Peer Reviewed
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[Presentation] Effects of Transcutaneous Application of Carbon Dioxide on diabetic muscle atrophy2019
Author(s)
Kondo H, Tani T, Naito K, Niimi Y, Nakane M, Sugiyama Y, Okayasu K, Takemoto H, Matsumoto T, Takuwa M, Hirabayashi T Ikeji T, Ono K, Fujino H.
Organizer
Experimental Biology
Related Report
Int'l Joint Research
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[Presentation] Effects of Transcutaneous Application of Carbon Dioxide on diabetic muscle atrophy2018
Author(s)
Kondo H, Tani T, Naito K, Niimi Y, Nakane M, Sugiyama Y, Okayasu K, Takemoto H, Matsumoto T, Takuwa M, Hirabayashi T Ikeji T, Ono K, Fujino H
Organizer
Experimental Biology (San Diego)
Related Report
Int'l Joint Research
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