Importance of postexercise calcium ion dynamics to regulate the adaptation of myocytes

• Project/Area Number: 16H03240
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Sports science
• Research Institution: The University of Electro-Communications
• Principal Investigator: Kano Yutaka 電気通信大学, 大学院情報理工学研究科, 教授 (90293133)
• Project Period (FY): 2016-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000); Fiscal Year 2019: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2018: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2017: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2016: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
• Keywords: 骨格筋 / カルシウムイオン / 可塑性 / 性差 / 筋収縮 / 熱ストレス / タンパク質合成 / エキセントリック収縮 / 廃用性筋萎縮 / 糖尿病 / 細胞内カルシウムイオン / 筋損傷 / 筋肥大 / バイオイメージング / in vivo / 生理学

Outline of Final Research Achievements

The mechanism of skeletal muscle adaptation to maintain muscle mass and strength is not fully understood. This study investigated the possibility that intracellular calcium ion controls muscle mass and muscle function. It was revealed that intracellular calcium ion changes continuously for a while after muscle contractions. These changes were different depending on the type of exercise stress and male/female. The characteristic calcium ion dynamics may control skeletal muscle mass due to exercise stress.

Academic Significance and Societal Importance of the Research Achievements

加齢や寝たきりなどの不活動状態，あるいは代謝的疾患（糖尿病など）に関連した筋肉量の減少は，Quality of Life(QOL)や健康寿命などに直結するリスクファクターである．本研究によって解明された運動時のカルシウムイオン変動パターンは，筋肉量を維持するための運動トレーニングやリハビリテーションのプログラム開発への応用が期待される．

Research Products

• [Int'l Joint Research] カンザス州立大学/ユタ州立大学(米国)
• [Int'l Joint Research] Kansas stete University/University of Utah School of Medicine(米国)
• [Int'l Joint Research] カンザス州立大学(米国)
• [Int'l Joint Research] カンザス州立大学(米国)
• [Journal Article] Sex differences in mitochondrial Ca2+ handling in mouse fast-twitch skeletal muscle in vivo (2020)
  • Author(s): Watanabe Daiki、Hatakeyama Koji、Ikegami Ryo、Eshima Hiroaki、Yagishita Kazuyoshi、Poole David C.、Kano Yutaka
  • Journal Title: Journal of Applied Physiology Volume: 128 Issue: 2 Pages: 241-251
  • DOI: 10.1152/japplphysiol.00230.2019
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Accumulation of intramyocyte TRPV1-mediated calcium during heat stress is inhibited by concomitant muscle contractions (2019)
  • Author(s): Ikegami Ryo、Eshima Hiroaki、Mashio Takuro、Ishiguro Tomosada、Hoshino Daisuke、Poole David C.、Kano Yutaka
  • Journal Title: Journal of Applied Physiology Volume: 126 Issue: 3 Pages: 691-698
  • DOI: 10.1152/japplphysiol.00668.2018
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Regional differences in Ca2+ entry along the proximal-middle-distal muscle axis during eccentric contractions in rat skeletal muscle (2019)
  • Author(s): Tabuchi Ayaka、Eshima Hiroaki、Tanaka Yoshinori、Nogami Shunsuke、Inoue Naoki、Sudo Mizuki、Okada Hidetaka、Poole David C.、Kano Yutaka
  • Journal Title: Journal of Applied Physiology Volume: 127 Issue: 3 Pages: 828-837
  • DOI: 10.1152/japplphysiol.01005.2018
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Muscle hypertrophy following blood flow-restricted low force isometric electrical stimulation in rat tibialis anterior: Role for muscle hypoxia. (2018)
  • Author(s): Nakajima T, Koide S, Yasuda T, Hasegawa T, Yamasoba T, Obi S, Toyoda S, Nakamura F, Inoue T, Poole DC, Kano Y
  • Journal Title: J Appl Physiol Volume: 印刷中 Issue: 1 Pages: 134-145
  • DOI: 10.1152/japplphysiol.00972.2017
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Microvascular Permeability of Skeletal Muscle After Eccentric Contraction-Induced Muscle Injury: In Vivo Imaging Using Two-Photon Laser Scanning Microscopy (2018)
  • Author(s): Kazuki Hotta, Bradley Behnke, Kazuto Masamoto, Rie Shimotsu, Naoya Onodera, Akihiko Yamaguchi, David Poole, Yutaka Kano
  • Journal Title: Journal of Applied Physiology Volume: 125 Issue: 2 Pages: 369-380
  • DOI: 10.1152/japplphysiol.00046.2018
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Improved skeletal muscle Ca2+ regulation in vivo following contractions in mice overexpressing PGC-1α. (2017)
  • Author(s): Eshima, H., Miura, S., Senoo, N., Hatakeyama, K., Poole, D.C., and Kano, Y.
  • Journal Title: Am J Physiol Regul Integr Comp Physiol Volume: 印刷中 Issue: 6 Pages: 1017-1028
  • DOI: 10.1152/ajpregu.00032.2017
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] In vivo Ca2+ dynamics induced by Ca2+ injection in individual rat skeletal muscle fibers (2016)
  • Author(s): Mario Wakizaka, Hiroaki Eshima, Yoshinori Tanaka, Hideki Shirakawa, David C. Poole, Yutaka Kano
  • Journal Title: Physiological Reports Volume: 5 Issue: 5
  • DOI: 10.14814/phy2.13180
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] pH buffering of single rat skeletal muscle fibers in the in vivo environment. (2016)
  • Author(s): Yoshinori Tanaka, Tadakatsu Inagaki, David C. Poole and Yutaka Kano.
  • Journal Title: American Journal of Physiology - Regulatory, Integrative and Comparative Physiology. Volume: in press Issue: 10 Pages: R926-R933
  • DOI: 10.1152/ajpregu.00501.2015
  • Peer Reviewed / Int'l Joint Research
• [Presentation] バイオイメージング科学の筋細胞研究への応用 (2019)
  • Author(s): 狩野 豊
  • Organizer: 第74回 日本体力医学会大会 つくば
  • Invited
• [Presentation] 損傷再生過程における細胞内カルシウムイオンダイナミクスとmTOR系の変化 (2018)
  • Author(s): 田渕絢香，白川英樹，芝口翼，杉浦崇夫，狩野豊
  • Organizer: 第73回 日本体力医学会 学会大会 福井
• [Presentation] 熱ストレスおよび筋収縮が筋細胞質内カルシウムイオン濃度に及ぼす影響 (2018)
  • Author(s): 池上諒，江島弘晃，星野太佑，狩野豊
  • Organizer: 第73回 日本体力医学会 学会大会 福井
• [Presentation] In vivo intracellular Ca2+ dynamics over 7 days following novel eccentric contractions in rat skeletal muscle (2017)
  • Author(s): Ayaka Tabuchi, Hideki Shirakawa, David C Poole, Yutaka Kano
  • Organizer: 第64回アメリカスポーツ医学会
  • Int'l Joint Research
• [Presentation] 伸張性筋収縮後に観察される筋細胞内Ca2+ダイナミクス (2016)
  • Author(s): 田渕絢香，狩野豊
  • Organizer: 第71回日本体力医学会
  • Place of Presentation: 市民文化ホール（岩手県盛岡市）
  • Year and Date: 2016-09-23
• [Remarks] 電気通信大学 基盤理工学専攻 化学生命工学プログラム 狩野研究室
  • URL: http://www.ecc.es.uec.ac.jp/index.html
• [Remarks] Research Highlights: In vivo bioimaging
  • URL: http://www.ru.uec.ac.jp/e-bulletin/research-highlights/2020/in-vivo-bioimaging-technology-to-elucidate-sex-dependent-differences-in-skeletal-muscle-function.html
• [Remarks] 電気通信大学 基盤理工学専攻 狩野研究室
  • URL: http://www.ecc.es.uec.ac.jp/index.html
• [Remarks] 電気通信大学狩野研究室ホームページ
  • URL: http://www.ecc.es.uec.ac.jp/index.html