| Budget Amount *help |
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2018: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2017: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2016: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
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| Outline of Final Research Achievements |
Development of new techniques for artificial regulation of specific cells in our body is one of essential technologies in life science researches. We have recently reported a new method for artifical activation of ion-channel-type glutamate receptors using coordination chemistry in live cells. In this method, two histidine mutations were introduced near the ligand-binding domain. Addition of Pd(bpy) complex, which coordinates to the histidine residues in a bidentate fashion, lead to stabilize the active conformation of the receptors for allosteric activation. Here, we successfully expanded our method for artificial regulation of G-protein coupled receptors (GPCRs). This means that our method is useful for artificial regulation of many kinds of receptors in live cells.
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