Active guidance and control in regenerative transplantation treatment for central nervous network with the aid of three-dimensional micro-dynamic stimulations
| Project/Area Number |
16H04289
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dynamics/Control
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| Research Institution | Yamagata University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
馮 忠剛 山形大学, 大学院理工学研究科, 准教授 (10332545)
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| Project Period (FY) |
2016-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2018: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2017: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2016: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
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| Keywords | iPS細胞 / 分化・成長誘導制御 / ３次元ゲル包埋培養 / 超小型３次元振動ステージ / ３次元動的力学刺激 / 中枢神経細胞 / ３次元ニューロンネットワーク / 移植モデル / 分化・成長誘導 / 超小型３Ｄ振動ステージ / ３次元神経回路網 / ニューロン簡易移植モデル / ３Ｄ動的マイクロ力学刺激 / 移植性促進法 / 3D振動ステージ / 3D動的力学刺激 / 3D神経回路網構築 / 再生移植モデル / ３次元神経回路網構築 / ３次元動的力学刺激環境 / 移植性促進法開発 / 3D中枢神経回路網 / ゲル包埋培養法 / 移植適合性評価 / ニューロンネットワーク / ３Ｄ動的力学刺激 / ３Ｄ振動ステージ / 3Dマイクロアクチュエータ / 脊髄再生移植モデル |
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| Outline of Final Research Achievements |
A novel small sized bimorph-piezoelectric cantilever type 3-D vibration table is developed to impose 3-D dynamic stimulations on cultured living cells in order to support regenerative transplantation treatment for central nervous network with the aid of iPS cells. Together with original gel-embedded 3-D culture, these devices provide us active guidance, control and construction of 3-D cultured central nervous networks.
A simulated transplantation treatment model of spinal cord injury is developed by utilizing the constructed 3-D central nervous networks. Using the developed 3-D vibration table, appropriate 3-D dynamic stimulations are applied upon the transplantation treatment model to promote engraftment and fusion of the neurons, and that usefulness of the present study as a supporting means for the neuronal regenerative transplantation treatment has been confirmed.
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| Academic Significance and Societal Importance of the Research Achievements |
再生移植治療は，人類の医療の行方のみならず人間の生き方をも左右する重要な技術で，早期実用化が切望されている。本研究で開発を目指した３次元動的マイクロ力学刺激付加デバイスシステムによるiPS細胞からの中枢神経回路網立体誘導制御，移植適合性事前評価法は，脊髄損傷，パーキンソン病，ALSなど重篤な神経系難病の治療法開発に新たな糸口をもたらす点で，学術的にも社会的にも極めて意義が大きい。その根幹をなす「３次元動的力学刺激環境のアクティブ応用」という技術は今後，細胞，組織，臓器レベルにわたる全く新たな次元の操作，制御，計測，治療のための手段となり得る可能性を秘めている点で極めて大きな波及効果を有する。
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Report
(4 results)
Research Products
(17 results)
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[Journal Article] Fine control of endothelial VEGFR-2 activation: caveolae as fluid shear stress shelters for membrane receptors2019
Author(s)
Shin, H, Haga, J.H., Kosawada, T., Kimura, K., Li, Y.S., Chien, S., Schmid-Schönbein, G.W.
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Journal Title
Biomechanics and Modeling in Mechanobiology
Volume: 18
Issue: 1
Pages: 5-16
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] Biomechanics of Collagen-Based Hydrogels as Tissue Engineering Scaffolds,2016
Author(s)
Z. Feng, Y. K. Fujita, T. Kosawada, D. Sato, T. Nakamura, Y. Shiraishi, T. Kitajima, M. Umezu
Organizer
38th Annual International Conference of the IEEE Engineering in Medicine & Biology Society
Place of Presentation
Orlando, FL, USA.
Year and Date
2016-08-16
Related Report
Int'l Joint Research
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