Avian transgenic technology against influenza
Project/Area Number |
16H04574
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Biofunction/Bioprocess
|
Research Institution | Nagoya University |
Principal Investigator |
|
Co-Investigator(Kenkyū-buntansha) |
小野 悦郎 九州大学, 医学研究院, 教授 (00160903)
|
Project Period (FY) |
2016-04-01 – 2020-03-31
|
Project Status |
Completed (Fiscal Year 2019)
|
Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2019: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2017: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
|
Keywords | ニワトリ / 生殖細胞 / ゲノム編集 / ウイルス / インフルエンザ / 始原生殖細胞 / 抗ウイルス / 応用動物 / 感染症 / IFITM10 / ウイルス感染性 / 畜産学 |
Outline of Final Research Achievements |
It was shown that chicken IFITM10 slightly reduced the infectivity of VSV-G-pseudotyped virus. In addition, overexpression of Muc13, of which gene size is below the limit to construct an expression vector, did not prevent infectivity of VSV-G-pseudotyped virus. Thus, expression system for large size mucin is under construction. We examined the gene transfer and genome editing conditions suitable for the long-term cultured primordial germ cells. Using CRISPR/Cas9, we succeeded in producing a genome-edited chicken in which the EGFP gene was knocked in.
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Academic Significance and Societal Importance of the Research Achievements |
本研究では、長期培養した始原生殖細胞を用いることにより、ゲノム編集を含めたニワトリの遺伝子改変を効率よく行えることが示された。一方、抗ウイルス活性が期待できる遺伝子候補の解析を進めつつあり、これらを融合してニワトリでのインフルエンザ流行抑制技術へつなげてゆくことが期待できる。逆に、ウイルス感受性が特に高いニワトリを作製し、侵入したばかりのウイルスを感度良く検出するために利用するなども期待できる。
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Report
(5 results)
Research Products
(18 results)