Single cell proteomics to molecular characterization of cancer stem cells
Project/Area Number |
16H04730
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
System genome science
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Research Institution | Kyushu University |
Principal Investigator |
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Research Collaborator |
Nakayama Keiichi
Bamba Takeshi
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Project Period (FY) |
2016-04-01 – 2019-03-31
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Project Status |
Completed (Fiscal Year 2018)
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Budget Amount *help |
¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Fiscal Year 2018: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2016: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
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Keywords | プロテオミクス / 1細胞分析 / がん幹細胞 / シングルセル / プロテオーム / シングルセル解析 / バイオテクノロジー |
Outline of Final Research Achievements |
We have developed a new targeted proteomics platform―in vitro proteome assisted MRM for protein absolute quantification (iMPAQT)― using >18,000 human recombinant proteins for genome-wide protein absolute quantification (Matsumoto et al., Nature Methods, 2017). Improvements of sample preparation and sample loading system enable us to measure proteome in very small amount of sample. We found that a cell cycle regulator, p57 protein is a good marker for cancer stem cell. On the basis of the expression of p57, cancer stem cells were effectively enriched. Finally, we performed multi-omics approach to identify the molecular signature of cancer stem cells.
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Academic Significance and Societal Importance of the Research Achievements |
本研究によって次世代型の定量プロテオミクスの技術基盤を確立することができた。これは、汎用的に利用されている手法と比べて精度や特異性において優れており、生命科学におけるタンパク質の解析に革新をもたらすことが期待される。さらに、高感度化によって小数細胞プロテオーム解析を実現可能としたことで、がん幹細胞の特徴を分子レベルで捉えることができるようなったことは、がんの根治を目指す医学研究を大いに促すものである。
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Report
(4 results)
Research Products
(26 results)
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[Journal Article] PKM1 Confers Metabolic Advantages and Promotes Cell-Autonomous Tumor Cell Growth.2018
Author(s)
M Morita, T Sato, M Nomura, Y Sakamoto, Y Inoue, R Tanaka, S Ito, K Kurosawa, K Yamaguchi, Y Sugiura, H Takizaki, Y Yamashita, R Katakura, I Sato, Y Okada, H Watanabe, G Kondoh, et al.
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Journal Title
Cancer Cell
Volume: 12
Issue: 3
Pages: 355-367
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Small Nucleolar Noncoding RNA SNORA23, Upregulated in Human Pancreatic Ductal Adenocarcinoma, Regulates Expression of SYNE2 to Promote Growth and Metastasis of Xenograft Tumors in Mice.2017
Author(s)
Cui L, Nakano K, Obchoei S, Setoguchi K, Matsumoto M, Yamamoto T, Obika S, Shimada K, Hiraoka N.
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Journal Title
Gastroenterology
Volume: 印刷中
Issue: 1
Pages: 292-306.e2
DOI
Related Report
Peer Reviewed
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[Journal Article] A large-scale targeted proteomics assay resource based on an in vitro human proteome.2017
Author(s)
Matsumoto M, Matsuzaki F, Oshikawa K, Goshima N, Mori M, Kawamura Y, Ogawa K, Fukuda E, Nakatsumi H, Natsume T, Fukui K, Horimoto K, Nagashima T, Funayama R, Nakayama K, Nakayama KI.
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Journal Title
Nat Methods
Volume: 14
Issue: 3
Pages: 251-258
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] jPOSTrepo: an international standard data repository for proteomes.2017
Author(s)
Okuda S, Watanabe Y, Moriya Y, Kawano S, Yamamoto T, Matsumoto M, Takami T, Kobayashi D, Araki N, Yoshizawa AC, Tabata T, Sugiyama N, Goto S, Ishihama Y.
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Journal Title
Nucleic Acids Res.
Volume: 45
Issue: D1
Pages: D1107-D1111
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] GRWD1 negatively regulates p53 via the RPL11-MDM2 pathway and promotes tumorigenesis.2017
Author(s)
Kayama K, Watanabe S, Takafuji T, Tsuji T, Hironaka K, Matsumoto M, Nakayama KI, Enari M, Kohno T, Shiraishi K, Kiyono T, Yoshida K, Sugimoto N, Fujita M.
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Journal Title
EMBO Rep.
Volume: 18
Issue: 1
Pages: 123-137
DOI
Related Report
Peer Reviewed
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[Journal Article] The novel heart-specific RING finger protein 207 is involved in energy metabolism in cardiomyocytes.2016
Author(s)
Mizushima, W., Takahashi , H., Watanabe, M., Kinugawa, S., Matsushima, S., Takada, S., Yokota, T., Furihata, T., Matsumoto, J., Tsuda, M., Chiba, I., Nagashima, S., Yanagi, S., Matsumoto, M., Nakayama, K.I., Tsutsui, H. and Hatakeyama, S.
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Journal Title
J. Mol. Cell. Cardiol.
Volume: 100
Pages: 43-53
DOI
NAID
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Ribophorin II is involved in the tissue factor expression mediated by phosphatidylserine-dependent antiprothrombin antibody on monocytes2016
Author(s)
Fujieda Y., Amengual O., Matsumoto M., Kuroki K., Takahashi H., Kono M., Kurita Y., Otomo K., Kato M., Oku K., Bohgaki T., Horita T., Yasuda S., Maenaka K., Hatakeyama S., Nakayama K.I. and Atsumi T. et al.
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Journal Title
Rheumatology
Volume: 印刷中
Issue: 6
Pages: 1117-1126
DOI
Related Report
Peer Reviewed / Open Access
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