The basic principles of RNA recognition mechanims of RNA modification enzymes
Project/Area Number |
16H04763
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Functional biochemistry
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Research Institution | Ehime University |
Principal Investigator |
HORI HIROYUKI 愛媛大学, 理工学研究科(工学系), 教授 (20256960)
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Co-Investigator(Kenkyū-buntansha) |
竹本 千重 国立研究開発法人理化学研究所, 生命機能科学研究センター, 副チームリーダー (40306527)
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Research Collaborator |
Hirata Akira
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Project Period (FY) |
2016-04-01 – 2019-03-31
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Project Status |
Completed (Fiscal Year 2018)
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Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2018: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2016: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
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Keywords | タンパク質 / 酵素 / 核酸 / RNA / ゲノム / 構造 |
Outline of Final Research Achievements |
The aim of this research is the clarifications of substrate RNA recognition mechanisms of RNA modification enzymes. The findings have been reported as follows. (1) THUMP domain, a RNA binding domain, binds to a single-stranded region of substrate RNA. In the cases of tRNA modification enzymes, it often recognizes the CCA-terminus of tRNA. (2) The C-terminal domain of thermostable TrmB, a tRNA methyltransferase, forms an S-adenosyl-L-methionine binding pocket and a part of the domain contact with the substrate tRNA. (3) In the network between modified nucleosides in tRNA and tRNA modification enzymes from extremetermophilic bacterium, TrmFO, a folate-and FAD-dependent tRNA methyltrnasfearse supports the adaptation for low-temperatures.
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Academic Significance and Societal Importance of the Research Achievements |
RNA修飾の基本的役割はタンパク質合成系の制御です。ヒトの場合、その制御が乱れると遺伝病や発がんに結びつくことが知られています。また、コレラ菌、チフス菌、ヒト免疫不全ウイルスなどにとって、RNA修飾は感染必須因子です。さらに、RNA修飾が環境(ストレス)応答因子であることも明らかとなりつつあります。したがって、どのようなRNA修飾酵素がどのようなRNAに作用するのか、また、いかに制御されているのかを理解することは、タンパク質合成系の基本制御の仕組みを解明し、遺伝病の病態の理解、遺伝子診断・遺伝子治療法の開発、感染性微生物対策、生物多様性の理解、環境応答の仕組みの解明などにつながります。
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Report
(4 results)
Research Products
(40 results)
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[Journal Article] Long and branched polyamines are required for maintenance of the ribosome, tRNAHis and tRNATyr in Thermus thermophilus cells at high temperatures2017
Author(s)
M. Nakashima, R. Yamagami, C. Tomikawa, Y. Ochi, T. Moriya, H. Asahara, D. Fourmy, S. Yoshizawa, *T. Oshima, and *H. Hori
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Journal Title
Genes to Cells
Volume: 22
Issue: 7
Pages: 628-645
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Long and branched polyamines are required for maintenance of the ribosome, tRNAHis, and tRNATyr in Thermus thermophilus cells at high temperatures2017
Author(s)
M. Nakashima, R. Yamagami, C. Tomikawa, Y. Ochi, T. Moriya, H. Asahara, D. Fourmy, S. Yoshizawa, T. Oshima and H. Hori
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Journal Title
Genes to Cells
Volume: 印刷中
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] The folate/FAD-dependent tRNA methyltransferase (TrmFO) from Thermus thermophilus regulates the other modifications in tRNA at low temperatures2016
Author(s)
R. Yamagami, C. Tomikawa, N. Shigi, A. Kazayama, S. Asai, H. Takuma, A. Hirata, D. Fourmy, H. Asahara, K. Watanabe, S. Yoshizawa and H. Hori
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Journal Title
Genes to Cells
Volume: 21
Issue: 7
Pages: 740-754
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Presentation] Long and branced polyamines are required for maintenance of 70S ribosome, tRNAHis, and tRNATyr in Thermus thermophilus2018
Author(s)
Nakashima, M., Yamagami, R., Tomikawa, C., Ochi, Y., Moriya, T., Asahara, H., Fourmy, D., Yoshizawa, S., Oshima, T. and Hori, H.
Organizer
27th tRNA Conference
Related Report
Int'l Joint Research
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[Presentation] The long and branched polyamines of Thermus thermophilus, an extremely thermophilic eubacterium, are required for maintenance of ribosome at high temperatures.2016
Author(s)
Misa Nakashima, Ryota Yamagami, Yuki Ochi, Chie Tomikawa, Toshiyuki Moriya, Dominique Fourmy, Satoko Yoshizawa, Tairo Oshima, and Hiroyuki Hori
Organizer
Extremophiles 2016
Place of Presentation
Kyoto University, Kyoto
Year and Date
2016-09-12
Related Report
Int'l Joint Research
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