• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Regulatory mechanisms of tannin accumulation in persimmon fruit and construction of a new innovative breeding strategy using these mechanisms

Research Project

Project/Area Number 16H04876
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Horticultural science
Research InstitutionRyukoku University

Principal Investigator

Yonemori Keizo  龍谷大学, 農学部, 教授 (10111949)

Co-Investigator(Kenkyū-buntansha) 佐藤 明彦  国立研究開発法人農業・食品産業技術総合研究機構, 果樹茶業研究部門, ユニット長 (30355440)
神崎 真哉  近畿大学, 農学部, 准教授 (20330243)
山根 久代  京都大学, 農学研究科, 准教授 (80335306)
Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2019: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2018: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2017: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Keywords園芸学 / 果樹 / 渋味 / 果実生理 / 育種 / 果樹園芸学
Outline of Final Research Achievements

The AST locus controlling persimmon astringency was determined by comparative mapping with Diospyros lotus, a diploid relative of D. kaki, as a reference. The syntenic region of AST locus was 915-kb in physical map of D. lotus. In addition, transcriptome and coexpression network analyses between PCNA and non-PCNA progenies revealed that AST gene affects a few transcription factors centered in the PA regulatory network. On the other hand, transcriptome analysis of the gene conferring non-astringent trait in Chinese-type PCNA persimmon indicated that the genes conferring sugar transporter, flavonoid glucosyltransferase, and flavonoid/phenylplopaloid O-methyltransferase may associate with this trait. Finally, a new innovative strategy was considered for PCNA persimmon breeding including Chinese-type PCNA persimmons.

Academic Significance and Societal Importance of the Research Achievements

タンニンの骨格となるプロアントシアニジン(PA)はカキに限らず、ブドウ、モモ、リンゴなどのさまざまな果樹に存在しており、機能性成分として重要な化合物である。本研究ではこの生合成を制御するAST遺伝子座をほぼ特定することができ、AST遺伝子単離の道筋が示され、今後、果実のPA生合成を制御できる可能性が開けた。このことは、学術的にも実用的にも大きな意義がある。また、カキの育種においても、これまでは完全甘ガキ形質が潜性(劣性)形質であるとの固定観念から育種戦略が構築されたが、中国の完全甘ガキも利用した新育種戦略構築の可能性が示されたことの意義も大きい。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (9 results)

All 2019 2018 2017 2016

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (7 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Comparative Mapping of the <i>ASTRINGENCY</i> Locus Controlling Fruit Astringency in Hexaploid Persimmon (<i>Diospyros kaki</i> Thunb.) with the Diploid <i>D. lotus</i> Reference Genome2018

    • Author(s)
      Nishiyama, S., N. Onoue, A. Kono, A. Sato, K. K. Ushijima, H. Yamane, , R. Tao, and K. Yonemori
    • Journal Title

      The Horticulture Journal

      Volume: 87 Issue: 3 Pages: 315-323

    • DOI

      10.2503/hortj.OKD-140

    • NAID

      130007420593

    • ISSN
      2189-0102, 2189-0110
    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Characterization of a gene regulatory network underlying astringency loss in persimmon fruit2017

    • Author(s)
      Nishiyama, S., N. Onoue, A. Kono, A. Sato, K. Yonemori, and R. Tao
    • Journal Title

      Planta

      Volume: 247 Issue: 3 Pages: 733-743

    • DOI

      10.1007/s00425-017-2819-0

    • NAID

      120006892318

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Presentation] クリプトーム解析による日本タイプ完全甘と中国タイプ完全甘のPA蓄積制御メカニズムの比較2019

    • Author(s)
      西山総一郎・尾上典之・河野淳・佐藤明彦・田尾龍太郎・米森敬三
    • Organizer
      園芸学会平成31年度春季大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] カキの果実発達を制御する遺伝子ネットワーク2019

    • Author(s)
      西山総一郎・尾上典之・河野淳・佐藤明彦・米森敬三・田尾龍太郎
    • Organizer
      園芸学会令和元年度秋季大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] トランスクリプトーム解析による日本タイプ完全甘と中国タイプ完全甘のPA蓄積制御メカニズムの比較2019

    • Author(s)
      西山総一郎・尾上典之・河野淳・佐藤明彦・田尾龍太郎・米森敬三
    • Organizer
      園芸学会平成31年度春季大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] カキ甘渋性決定遺伝子座の多様性解析2018

    • Author(s)
      西山総一郎・藏本晃栄・尾上典之・河野淳・佐藤明彦・米森敬三・田尾龍太郎
    • Organizer
      園芸学会平成30年度秋季大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] カキ甘渋性に関する共発現ネットワーク解析2017

    • Author(s)
      西山総一郎、尾上典之、河野 淳、佐藤明彦、米森敬三、田尾龍太郎
    • Organizer
      園芸学会平成29年度春季大会研究発表会
    • Place of Presentation
      日本大学生物資源学部
    • Year and Date
      2017-03-19
    • Related Report
      2016 Annual Research Report
  • [Presentation] カキ甘渋性決定遺伝子座とシンテニィのあるマメガキゲノム領域の解析2017

    • Author(s)
      西山総一郎・尾上典之・河野淳・佐藤明彦・牛島幸一郎・山根久代・田尾龍太郎・米森敬三
    • Organizer
      園芸学会平成29年度秋季大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Transcriptome analysis for astringency in persimmon fruit2016

    • Author(s)
      Nishiyama, S., A. Kono, A. Sato, K. Yonemori, and R. Tao
    • Organizer
      VI International Symposium on Persimmon
    • Place of Presentation
      Valencia, Spain
    • Year and Date
      2016-10-17
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research

URL: 

Published: 2016-04-21   Modified: 2021-02-19  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi