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Analysis of the possibility of siderophore-mediated silicon uptake

Research Project

Project/Area Number 16H04900
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied microbiology
Research InstitutionHiroshima University

Principal Investigator

Ikeda Takeshi  広島大学, 先端物質科学研究科, 助教 (10505754)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2018: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
Keywords細菌 / 胞子 / ケイ素 / 応用微生物 / 生体分子
Outline of Final Research Achievements

Bacillus cereus and its close relatives take up soluble silicate from the environment and accumulate it as insoluble silica in and around the spore coat layer. However, the mechanism underlying silicate uptake has not been clarified. Recently, it has been reported that siderophores, natural iron-chelating compounds secreted by microorganisms, can form a complex with silicon. We hypothesized that siderophores might be involved in silicate uptake in B. cereus. The disruption of one of the two siderophore biosynthesis operons resulted in significant decrease in silicate uptake. However, medium exchange during cultivation did not affect silicate uptake, suggesting that siderophore-mediated transport is not a major route of silicate uptake.

Academic Significance and Societal Importance of the Research Achievements

シデロフォアを介したケイ酸の取り込みが証明できれば新奇な発見であったが、予想に反してケイ酸の取り込みそのものにはシデロフォアが関与していないことが示された。一方、シデロフォアを合成できない遺伝子破壊株ではケイ酸取り込み量が顕著に減少したことから、取り込まれたケイ酸が重合してシリカが形成されるプロセスのどこかでシデロフォアが何らかの役割を果たしている可能性が示唆された。シデロフォアの未知の機能の発見につながることが期待される。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (4 results)

All 2018 2017 2016

All Presentation (4 results) (of which Int'l Joint Research: 1 results)

  • [Presentation] ケイ素を胞子表面に蓄積するBacillus属細菌の解析と界面バイオテクノロジーへの応用2018

    • Author(s)
      池田 丈, 廣田 隆一, 黒田 章夫
    • Organizer
      第70回日本生物工学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ケイ素(Si)で胞子をコーティングするBacillus属細菌2017

    • Author(s)
      池田 丈, 廣田 隆一, 黒田 章夫
    • Organizer
      環境微生物系学会合同大会2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] Silica biomineralization in the gram-positive, spore-forming bacterium Bacillus cereus2017

    • Author(s)
      Takeshi Ikeda, Miki Nakagawa, Kei Motomura, Ryuichi Hirota, Akio Kuroda
    • Organizer
      BiominXIV/14th International Symposium on biomineralization
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Bacillus属細菌のシリカ蓄積メカニズムの解析2016

    • Author(s)
      池田 丈, 本村 圭, 田中 達也, 廣田 隆一, 黒田 章夫
    • Organizer
      環境バイオテクノロジー学会2016年度大会
    • Place of Presentation
      広島県民文化センター
    • Year and Date
      2016-06-13
    • Related Report
      2016 Annual Research Report

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Published: 2016-04-21   Modified: 2020-03-30  

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