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The Growth Strategy of Tricholoma matsutake colony with an antimicrobial complex, (oxalato)auyminium

Research Project

Project/Area Number 16H04935
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Forest science
Research InstitutionKyoto University

Principal Investigator

Hirai Nobuhiro  京都大学, 農学研究科, 教授 (00165151)

Co-Investigator(Kenkyū-buntansha) 山田 明義  信州大学, 学術研究院農学系, 准教授 (10324237)
山口 宗義  国立研究開発法人森林研究・整備機構, 森林総合研究所, 主任研究員 等 (20353899)
服部 武文  徳島大学, 大学院社会産業理工学研究部(生物資源産業学域), 准教授 (60212148)
田中 千尋  京都大学, 農学研究科, 教授 (60263133)
Research Collaborator Fujita Toru  
Sakamoto Yuichi  
Narimatsu Masaki  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2018: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2017: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2016: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Keywordsマツタケ / 抗菌物質 / シュウ酸アルミニウム / 菌根 / アカマツ / リン酸
Outline of Final Research Achievements

The mycorrhizal region of Tricholoma matsutake is called shiro, and fruiting bodies occur at the periphery. It has been known from the 1960s that the front of shiro (active mycorrhizal zone) produced an antibacterial substance, but the substance remained unknown. The applicant has recently found that the substance is an aluminum oxalate complex. In this study, we confirmed the distribution and the seasonal changes in content of the complex in shiro, the distribution of soil microbes in shiro, and the occurrence of the complex in shiro of various places, and revealed that it plays an important strategy for the growth of shiro. In addition, identification of oxalic acid biosynthesis genes by T. matsutake was also attempted, and candidate genes were found. These results contribute to the conservation and vitality maintenance of village forest through the application to the cultivation technology of T. matsutake shiro.

Academic Significance and Societal Importance of the Research Achievements

マツタケの生産量は里山林の活用が低下した1960年代以降急激に減少している。マツタケの菌根領域はシロと呼ばれており、子実体はその周縁部で発生する。シロの先端が抗菌物質を生産していることは1960年代より知られていたが、本体は不明のままであった。申請者はその本体がシュウ酸アルミニウム錯体であることを解明し、同錯体がその生理・生態学的機能を通じてシロの成長に重要な役割を果たしていることを初めて明らかにした。従来のマツタケ研究は人工栽培など応用的なものが多かったが、本研究はシュウ酸アルミニウムに関する基礎研究であり、マツタケシロの育成技術への貢献が改めて期待されるものである。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (20 results)

All 2019 2018 2017 2016

All Journal Article (10 results) (of which Peer Reviewed: 10 results,  Acknowledgement Compliant: 1 results) Presentation (8 results) (of which Int'l Joint Research: 3 results) Book (2 results)

  • [Journal Article] 茶葉および茶畑土壌中のアルミニウム錯体濃度2019

    • Author(s)
      西野勝俊、久保中央、福永晃士、平井伸博
    • Journal Title

      茶業研究報告

      Volume: 126 Pages: 17-23

    • NAID

      130007965483

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Classification of tea (Camellia sinensis) landraces and cultivars in Kyoto, Japan and other regions, based on simple sequence repeat markers and restriction site-associated DNA sequencing analysis2019

    • Author(s)
      N. Kubo, Y. Mimura, T. Matsuda, A. J. Nagano, N. Hirai, S. Higashimoto, H. Yoshida, N. Uemura and T. Fujii
    • Journal Title

      Genetic Resources and Crop Evolution

      Volume: 66 Issue: 2 Pages: 441-451

    • DOI

      10.1007/s10722-018-0722-6

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Physiological variation among Tricholoma matsutake isolates generated from basidiospores obtained from one basidioma.2019

    • Author(s)
      Yamada A, Hayakawa N, Saito C, Horimai Y, Misawa H, Yamanaka T, Fukuda M.
    • Journal Title

      Mycoscience

      Volume: 60 Issue: 2 Pages: 102-109

    • DOI

      10.1016/j.myc.2018.12.001

    • NAID

      120007183591

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 抗菌物質・シュウ酸アルミニウムのマツタケシロにおける普遍的存在2018

    • Author(s)
      西野勝俊, 山口宗義, 藤田 徹, 東 智則, 宜寿次盛生, 成松眞樹, 山田明義, 平井伸博
    • Journal Title

      きのこ学会誌

      Volume: 26 Pages: 24-27

    • NAID

      130007753863

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In vitro ectomycorrhization of Tricholoma matsutake strains is differentially affected by soil type2018

    • Author(s)
      Saito C, Ogawa W, Kobayashi H, Yamanaka T, Fukuda M, Yamada A.
    • Journal Title

      Mycoscience

      Volume: 59 Issue: 1 Pages: 89-97

    • DOI

      10.1016/j.myc.2017.09.002

    • NAID

      120007183590

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Seasonal change in the content of the (oxalato)aluminate complex, an antimicrobial substance of the shiro of Tricholoma matsutake, and the bacterial community structure in the shiro area.2017

    • Author(s)
      K. Nishino, K. Matsubara, C. Tanaka, M. Yamaguchi, T. Fujita, A. Yamada and N. Hirai.
    • Journal Title

      Mushroom Science and Biotechnology

      Volume: 25 (1) Pages: 9-16

    • NAID

      130007489347

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The (oxalato)aluminate complex as an antimicrobial substance protecting the “shiro” of Tricholoma matsutake from soil micro-organisms2017

    • Author(s)
      Nishino, K.; Shiro, M.; Okura, R.; Oizumi, K.; Fujita, T.; Sasamori, T.; Tokitoh, N.; Yamada, A.; Tanaka, C.; Yamaguchi, M.; Hiradate, S.; Hirai, N.
    • Journal Title

      Biosci. Biotechnol. Biochem.

      Volume: 81 Issue: 1 Pages: 101-111

    • DOI

      10.1080/09168451.2016.1238298

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Endogone corticioides sp. nov. from subalpine conifer forests in Japan and China, and its multi-locus phylogeny2017

    • Author(s)
      Yamamoto K, Degawa Y, Takashima Y, Fukuda M, Yamada A.
    • Journal Title

      Mycoscience

      Volume: 58 Issue: 1 Pages: 23-29

    • DOI

      10.1016/j.myc.2016.08.001

    • NAID

      40021065760

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] First detection of Endogone ectomycorrhizas in natural oak forests2017

    • Author(s)
      Yamamoto K, Endo N, Degawa Y, Fukuda M, Yamada A
    • Journal Title

      Mycorrhiza

      Volume: 27 Issue: 3 Pages: 295-301

    • DOI

      10.1007/s00572-016-0740-1

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A qPCR assay that specifically quantifies Tricholoma matsutake biomass in natural soil2016

    • Author(s)
      Yamaguchi M, Narimatsu M, Fujita T, Kawai M, Kobayashi H, Ohta A, Yamada A, Matsushita N, Neda H, Shimokawa T, Murata H.
    • Journal Title

      Mycorrhiza

      Volume: 26 Issue: 8 Pages: 847-861

    • DOI

      10.1007/s00572-016-0718-z

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Presentation] マツタケ(Tricholoma matsutake)の菌根成長に対するシュウ酸の効果2018

    • Author(s)
      高橋翔, 山田明義, 平井伸博
    • Organizer
      日本きのこ学会第22回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] マツタケシロの抗菌物質・シュウ酸アルミニウムの濃度とマツタケ菌体量の季節変化2017

    • Author(s)
      西野勝俊、松原佳耶、田中千尋、山口宗義、藤田 徹、山田明義、平井伸博
    • Organizer
      森林学会第128回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] The (oxalato)aluminate complex as an antimicrobial substance protecting the “shiro” of Tricholoma matsutake from soil microorganisms2017

    • Author(s)
      Katsutoshi Nishino, Misao Shiro, Ryuki Okura, Kazuya Oizumi, Toru Fujita, Takahiro Sasamori, Norihiro Tokitoh, Akiyoshi Yamada, Kaya Matsubara, Chihiro Tanaka, Muneyoshi Yamaguchi, Syuntaro Hiradate, and Nobuhiro Hirai
    • Organizer
      The joint meeting of the 33rd annual meeting of the ISCE (International Society of Chemical Ecology) and the 9th meeting of the APACE (Asia and Pacific Association of Chemical Ecology)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] SSRおよびRADマーカーに基づく京都府内外チャ品種・在来系統の類縁関係2017

    • Author(s)
      久保中央,三村裕,松田智宏,永野惇,平井伸博,吉田浩実,植村則大,藤井孝夫
    • Organizer
      日本育種学会第131回講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Spore germination and ectomycorrhization of Tricholoma matsutake on a host pine root system in vitro.2016

    • Author(s)
      Horimai Y, Yamada A, Furukawa H, Masuno K, Yamashita S, and Yamanaka T.
    • Organizer
      8th International Workshop of Edible Mycorrhizal Mushrooms.
    • Place of Presentation
      フランス,カオール市
    • Year and Date
      2016-10-12
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Repeated Fruiting of Yellow Chanterelle in Pot Culture.2016

    • Author(s)
      Ogawa W, Yamashita S, and Yamada A
    • Organizer
      8th International Workshop of Edible Mycorrhizal Mushrooms.
    • Place of Presentation
      フランス,カオール市
    • Year and Date
      2016-10-12
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] マツタケ菌体量と抗菌物質・シュウ酸アルミニウム濃度の季節変化2016

    • Author(s)
      西野勝俊、松原果耶、田中千尋、山口宗義、藤田 徹、山田明義、平井伸博
    • Organizer
      2016年度日本菌学会年次大会
    • Place of Presentation
      京都市京都大学
    • Year and Date
      2016-09-17
    • Related Report
      2016 Annual Research Report
  • [Presentation] マツタケシロにおけるマツタケ菌体量と抗菌物質・シュウ酸アルミニウム濃度の季節変化2016

    • Author(s)
      西野勝俊,松原佳耶,田中千尋,山口宗義,藤田 徹、山田明義,平井伸博
    • Organizer
      日本きのこ学会第20回大会
    • Place of Presentation
      静岡市アザレア
    • Year and Date
      2016-09-08
    • Related Report
      2016 Annual Research Report
  • [Book] Biogeography of Mycorrhizal Symbiosis, Ecol. Stud. 2302017

    • Author(s)
      Vaario LM, Yang X, Yamada A.
    • Total Pages
      566
    • Publisher
      Springer, Berlin.
    • Related Report
      2017 Annual Research Report
  • [Book] 驚きの菌ワールド:菌類の知られざる世界2017

    • Author(s)
      日本菌学会編(外生菌根,pp38-39,山田明義・遠藤直樹・小川和香奈)
    • Total Pages
      89
    • Publisher
      東海大学出版
    • Related Report
      2016 Annual Research Report

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Published: 2016-04-21   Modified: 2020-03-30  

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