| Budget Amount *help |
¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000)
Fiscal Year 2018: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2017: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
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| Outline of Final Research Achievements |
A variety of microorganisms play a significant role in the mineralization of plant lignin. We have isolated a number of biphenyl/PCB-degrading bacteria from various environmental samples and studied the degradation modes from the biochemical and genetic bases. In this study, we found that some biphenyl/PCB-degrading bacteria can grow well on lignin-derived aromatic compounds as the sole sources of carbon and energy. Using gel-based proteomics, we found that many proteins responsible for the metabolism of aromatic compounds are inducibly expressed in a Pseudomonas strain. We also cloned the lignin peroxidase genes from a Rhodococcus strain and expressed them. The results show that using proteomic and genomic approaches, we can efficiently screen and identify candidate proteins and genes involved in the metabolism of lignin-derived aromatic compounds from biphenyl/PCB-degrading bacteria. These approaches can also lead to the production of useful aromatic compounds from wood biomass.
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