A comprehensive proteomic analysis for the Identification of factors contributing to hyaline cartilage regeneration
Project/Area Number |
16H05455
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Orthopaedic surgery
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Research Institution | Tokai University |
Principal Investigator |
SATO Masato 東海大学, 医学部, 教授 (10056335)
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Co-Investigator(Kenkyū-buntansha) |
豊田 恵利子 東海大学, 医学部, 特定研究員 (90749269)
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Project Period (FY) |
2016-04-01 – 2019-03-31
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Project Status |
Completed (Fiscal Year 2018)
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Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2018: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
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Keywords | タンパク質 / miRNA / マイクロアレイ解析 / 細胞シート / 再生医学 / マイクロアレイ / 蛋白質 |
Outline of Final Research Achievements |
To identify the mechanisms and anabolic factors that contribute to the hyaline cartilage regenerative properties of chondrocyte sheets, we performed a proteomic analysis (SOMAscan) of factors that vary in correlation with the cartilage regenerative properties of chondrocyte sheets fabricated under different culture conditions and identified 26 such factors. Through the analysis of the change in levels of gene expression and secreted factors of chondrocyte sheets with varying cartilage regenerative properties, regulators of Wnt signaling, regulators of inflammation, and growth factors were found to correlate with the cartilage regenerative properties. Furthermore, through the microarray analysis of exosomal miRNAs in culture supernatants, miRNAs that correlate with the cartilage regenerative properties were identified. Validation through RT-PCR revealed 4 such miRNAs.
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Academic Significance and Societal Importance of the Research Achievements |
軟骨細胞シートは、損傷した軟骨からのプロテオグリカンの流出の阻止や、関節液中の軟骨分解因子からの軟骨の保護に働き、軟骨成長因子の持続的な供給や、軟骨下骨から誘導される骨髄由来幹細胞に対する軟骨分化促進イニシエーターとしての機能等を介して軟骨修復を促進していると考えられるが、これらの作用にかかわる因子やメカニズムについては不明な点も多い。 自然には修復しない関節軟骨の再生に有用な因子の一端を明らかにした本研究成果は、軟骨細胞シートが軟骨修復を促進するメカニズムの解明に繋がるものである。
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Report
(4 results)
Research Products
(12 results)
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[Journal Article] Combined surgery and chondrocyte cell-sheet transplantation improves clinical and structural outcomes in knee osteoarthritis2019
Author(s)
Sato M, Yamato M, Mitani G, Takagaki T, Hamahashi K, Nakamura Y, Ishihara M, Matoba R, Kobayashi H, Okano T, Mochida J, Watanabe M
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Journal Title
NPJ Regen Med
Volume: 4
Issue: 1
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] 多指症由来軟骨細胞シートの拡大培養と特性変化の解析2019
Author(s)
豊田恵利子, 佐藤正人, 前原美樹, 高橋匠, 野中謙, 飯島寛, 的場亮, 川口佑加, 佐藤千香子, 高木岳彦, 阿久津英憲, 梅澤明弘, 赤松正, 渡辺雅彦
Organizer
第32回日本軟骨代謝学会
Related Report
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[Presentation] Analysis of micro-RNAs in exosomes secreted by human polydactyly-derived chondrocyte sheets2018
Author(s)
Toyoda E, Sato M, Maehara M, Takahashi T, Takagi T, Nonaka K, Iijima H, Matoba R, Akutsu H, Umezawa A, Akamatsu T, Watanabe M
Organizer
The 2018 Osteoarthritis Research Society International (OARSI) World Congress
Related Report
Int'l Joint Research
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[Presentation] Identification of altered gene expression in the expansion polydactyly-derived chondrocytes2018
Author(s)
Toyoda E, Sato M, Maehara M, Takahashi,T, Takagi T, Kotoku T, Sato C, Nonaka K, Matoba R, Akutsu H, Umezawa A, Akamatsu T, Watanabe M
Organizer
5th TERMIS World Congress 2018
Related Report
Int'l Joint Research
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[Presentation] Evaluation of Humoral Factors Produced by Polydactyly-derived Chondrocyte Sheets using SOMAscanTM2018
Author(s)
Takahashi T, Sato M, Toyoda E, Maehara M, Takizawa D, Sogo Y, Tominaga A, Okada E, Takagi T, Okazaki K, Akamatsu T, Watanabe M
Organizer
Orthopaedic Research Society 2018 Annual Meeting
Related Report
Int'l Joint Research
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[Presentation] ヒト軟骨細胞シートが産生するタンパク質の網羅的解析.2016
Author(s)
豊田恵利子, 佐藤正人, 高橋匠, 前原美樹, 高垣智紀, 浜橋恒介, 幸得友美, 岡田恵里, 渡部綾子, 佐藤千香子, 滝澤大智, 的場亮, 阿久津英憲, 梅澤明弘, 持田讓治.
Organizer
第31回日本整形外科学会基礎学術集会
Place of Presentation
福岡国際会議場(福岡県福岡市)
Year and Date
2016-10-13
Related Report
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