Elucidation of structural-functional relationships of genes related to teeth agenesis using reverse genetics
| Project/Area Number |
16H05551
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthodontics/Pediatric dentistry
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| Research Institution | The University of Tokushima |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
田中 栄二 徳島大学, 大学院医歯薬学研究部(歯学域), 教授 (40273693)
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| Project Period (FY) |
2016-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2019: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2018: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2017: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
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| Keywords | 歯の発生 / ゲノム編集 / in vivo DNA deletion / In vivo DNA deletion / CRISPR/Casシステム |
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| Outline of Final Research Achievements |
Msx1 is known as a causative gene for tooth agenesis and is also important for tooth development in mice. Its functional domains have been well studied using cultured cells, on the other hand, the analysis using mice has not been performed because the problem of the gene disruption technology and the cost. Then, we have tried functional analysis of each domain of the Msx1 gene in mice using the genome editing technology that has been developed in recent years. We found that the MH5 and MH6 domains downstream of the homeodomain are important for tooth and bone formation.
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| Academic Significance and Societal Importance of the Research Achievements |
組織・器官の発生過程における遺伝子機能の解析は、培養細胞系では困難な場合が多く、特に発生学研究の主役である転写因子の生体での解析には一定の歯止めがかかって来たのが現状である。これに伴い、ヒトで遺伝子変異が検出された場合も、本当に疾患責任変異かどうかの判定も困難な場合が多い。しかし、今回、動物を用いて検出変異の疾患責任性を検証することが可能とわかり、正しい遺伝子診断への展開も期待できるようになった。
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Report
(5 results)
Research Products
(27 results)
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[Journal Article] MicroRNAs control eyelid development through regulating Wnt signaling.2019
Author(s)
Nagai T, Trakanant S, Kawasaki M, Kawasaki K, Yamada Y, Watanabe M, Blackburn J, Otsuka-Tanaka Y, Hishinuma M, Kitatmura A, Meguro F, Yamada A, Kodama Y, Maeda T, Zhou Q, Saijo Y, Yasue A, Sharpe PT, Hindges R, Takagi R, Ohazama A.
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Journal Title
Dev Dyn
Volume: 248
Issue: 3
Pages: 201-210
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Somatic cell reprogramming-free generation of genetically modified pigs.2016
Author(s)
Tanihara F, Takemoto T, Kitagawa E, Rao S, Do LT, Onishi A, Yamashita Y, Kosugi C, Suzuki H, Sembon S, Suzuki S, Nakai M, Hashimoto M, Yasue A, Matsuhisa M, Noji S, Fujimura T, Fuchimoto D, Otoi T.
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Journal Title
Sci Adv
Volume: 14
Issue: 9
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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