Development of a new evaluation tool for measuring malaria infection risk based on antibody responses to Anopheles saliva protein
Project/Area Number |
16H05815
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 海外学術 |
Research Field |
Parasitology (including sanitary zoology)
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Research Institution | Kanazawa University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
伊従 光洋 金沢大学, 薬学系, 准教授 (20608351)
砂原 俊彦 長崎大学, 熱帯医学研究所, 助教 (50264156)
都野 展子 金沢大学, 自然システム学系, 准教授 (60295102)
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Project Period (FY) |
2016-04-01 – 2019-03-31
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Project Status |
Completed (Fiscal Year 2018)
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Budget Amount *help |
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2018: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
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Keywords | マラリア / ハマダラカ / 唾液タンパク / バイオマーカー / 抗体価 / 疫学調査 / インドネシア / 蚊唾液タンパク / 唾液タンパク質 |
Outline of Final Research Achievements |
The objective of the present study was to determine whether the IgG antibody response to the Anopheles stephensi saliva antigen, AAPP, is an immuno-epidemiological marker of exposure specific to Anopheles mosquito bites in the Sumba Islanders in Indonesia. Recombinant AAPP was produced in E. coli system. Anti-AAPP IgG prevalences and levels were significantly higher in the sera of the Sumba Islanders as compared to non-exposed Japanese controls. These observations show that the AAPP is immunogenic, evokes in exposed individuals an IgG antibody response. This initial test provided encouraging preliminary information on the immunogenicity of the AAPP protein; however, the E. coli-expressed AAPP generated a relatively high background in control sera (i.e. sera from individuals not exposed to Anopheles bites) even though it was apparently pure. We are trying to establish another protocol for recombinant AAPP using cell-free system without any trace of bacterial contamination.
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Academic Significance and Societal Importance of the Research Achievements |
マラリア感染地域住民のハマダラカ唾液に対する抗体価を季節毎(雨季乾季)に測定し、その季節変動とマラリア感染率との相関関係を解明することが可能となる。これにより、衛生動物学的手法と血清疫学的手法を融合でき、マラリアベクターコントロールの有効性を評価することに加えて再流行のリスク管理に有効な手法となる。さらには、インドネシア島嶼群のマラリア疫学調査および三日熱マラリアワクチンの抗原性評価研究成果をもって、日本―インドネシア二国間の永続的研究協力体制を確立することができる社会的意義がある。
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Report
(4 results)
Research Products
(48 results)
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[Journal Article] Adeno-Associated Virus as an Effective Malaria Booster Vaccine Following Adenovirus Priming2019
Author(s)
Yusuf Y, Yoshii T, Iyori M, Yoshida K, Mizukami H, Fukumoto S, Yamamoto DS, Alam A, Emran TB, Amelia F, Islam A, Otsuka H, Takashima E, Tsuboi T, Yoshida S.
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Journal Title
Frontiers in Immunology
Volume: 10
Pages: 730-730
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Adenovirus-prime and baculovirus-boost heterologous immunization achieves sterile protection against malaria sporozoite challenge in a murine model.2018
Author(s)
Yoshida K, Iyori M, Blagborough AM, Salman AM, Dulal P, Sala KA, Yamamoto DS, Khan SM, Janse CJ, Biswas S, Yoshii T, Yusuf Y, Tokoro M, Hill AVS, Yoshida S
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Journal Title
Scientific Reports
Volume: 8
Issue: 1
Pages: 3896-3896
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Baculovirus-Induced Fast-Acting Innate Immunity Kills Liver-Stage Plasmodium.2018
Author(s)
Emran TB, Iyori M, Ono Y, Amelia F, Yusuf Y, Islam A, Alam A, Tamura M, Ogawa R, Matsuoka H, Yamaoto DS, Yoshida S.
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Journal Title
The Journal of Immunology
Volume: 201
Issue: 8
Pages: 2441-2441
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Development of a novel malaria vaccine based on heterologous prime-boost immunization regimen using adenovirus and adeno-associated virus vectors2018
Author(s)
Iyori M, Yoshida K, Yusuf Y, Emran TB, Yoshii T, Hashizume H, Shimada S, Yamamoto DS, Fukumoto S, Mizukami H, Yoshida S
Organizer
14th International congress of parasitology
Related Report
Int'l Joint Research
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[Presentation] Plasmodium falciparum CSP vaccine based on a heterologous adenoviru-sprime and baculovirus-boost immunization regimen confers sterile protection against transgenic P.berghei sporozoite challenge2017
Author(s)
Kunitaka Yoshida, Mitsuhiro Iyori, Ahmed M. Salman, Pawan Dulal, Shahid M. Khan, Chris J. Janse, Sumi Biswas, Masaharu Tokoro, Andrew M. Blagborough, Adrian V.S. Hill, Shigeto Yoshida
Organizer
The U.S.‐Japan Cooperative Medical Sciences Program Presents the 19th International Conference on Emerging Infectious Diseases (EID)
Place of Presentation
韓国 ソウル
Year and Date
2017-02-07
Related Report
Int'l Joint Research
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[Presentation] Plasmodium falciparum CSP vaccine based on a heterologous adenovirus prime and baculovirus-boost immunization regimen confers sterile protection against transgenic P. berghei sporozoite challenge2017
Author(s)
Kunitaka Yoshida, Mitsuhiro Iyori, Ahmed M. Salman, Pawan Dulal, Shahid M. Khan, Chris J. Janse, Sumi Biswas, Masaharu Tokoro, Andrew M. Blagborough, Adrian V.S. Hill, Shigeto Yoshida
Organizer
The U.S.‐Japan Cooperative Medical Sciences Program Presents the 19th International Conference on Emerging Infectious Diseases (EID)
Related Report
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[Presentation] Enhanced protective efficacy of a P. falciparum malaria vaccine using a heterologous prime-boost immunization with a baculoviral vaccine and ChAd63 expressing PfCSP against challenge with a transgenic P. berghei sporozoites2016
Author(s)
Kunitaka Yoshida, Ahmed M. Salman, Pawan Dulal, Shahid M. Khan, Chris J. Janse, Sumi Biswas, Masaharu Tokoro, Mitsuhiro Iyori, Andrew M. Blagborough, Adrian V. S. Hill, Shigeto Yoshida
Organizer
ASTMH 65th Annual Meeting
Place of Presentation
アメリカ アトランタ
Year and Date
2016-11-13
Related Report
Int'l Joint Research
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[Presentation] Vectored PfCSP Vaccines based on Baculovirus Dual Expression System and AdHu5 induce Strong Protective Efficacy against Transgenic Plasmodium berghei2016
Author(s)
Mitsuhiro Iyori, Andrew M. Blagborough, Sota Ogata, Hidesato Nishiura, Miako Sakaguchi, Masanori Mizutani, Takahiko Tamura, Kento Genshi, Satoshi Shimada, Daisuke S. Yamamoto, Hiroyuki Matsuoka and Shigeto Yoshida
Organizer
ASTMH 65th Annual Meeting
Place of Presentation
アメリカ アトランタ
Year and Date
2016-11-13
Related Report
Int'l Joint Research
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[Presentation] A hybrid Plasmodium falciparum malaria vaccine based on adenovirus-prime and baculovirus-boost immunization regimen2016
Author(s)
Kunitaka Yoshida, Mitsuhiro Iyori, Ahmed M. Salman, Pawan Dulal, Shahid M. Khan, Chris J. Janse, Sumi Biswas, Masaharu Tokoro, Andrew M. Blagborough, Adrian V.S. Hill, Shigeto Yoshida
Organizer
第57回日本熱帯医学会大会
Place of Presentation
一橋大学
Year and Date
2016-11-05
Related Report
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[Presentation] アデノウイルスベクターとバキュロウイルスベクターを用いた新規ワクチンプラットフォームによる熱帯熱マラリアワクチンの開発2016
Author(s)
吉田邦嵩, Ahmed M. Salman, Pawan Dulal, Shahid M. Khan, Chris J. Janse, Sumi Biswas, 所正治, 伊従光洋, Andrew M. Blagborough, Adrian V.S. Hill, 吉田栄人
Organizer
第72回日本寄生虫学会西日本支部大会
Place of Presentation
岐阜大学
Year and Date
2016-10-15
Related Report
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[Presentation] A hybrid Plasmodium falciparum malaria vaccine based on adenovirus-prime and baculovirus-boost immunization regimen.2016
Author(s)
Kunitaka Yoshida, Ahmed M. Salman, Pawan Dulal, Shahid M. Khan, Chris J. Janse, Sumi Biswas, Masaharu Tokoro, Mitsuhiro Iyori, Andrew M. Blagborough, Adrian V. S. Hill, Shigeto Yoshida
Organizer
第14回松山国際学術シンポジウム
Place of Presentation
愛媛大学
Year and Date
2016-09-15
Related Report
Int'l Joint Research
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