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Novel microbial metabolism responsible for the carbon flux in deep subseafloor sediments as revealed by isolation and stable isotope probing

Research Project

Project/Area Number 16H05886
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Environmental dynamic analysis
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

Hori Tomoyuki  国立研究開発法人産業技術総合研究所, エネルギー・環境領域, 主任研究員 (20509533)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥24,310,000 (Direct Cost: ¥18,700,000、Indirect Cost: ¥5,610,000)
Fiscal Year 2018: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Fiscal Year 2017: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Fiscal Year 2016: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
Keywords環境分析 / 深海環境 / 地球化学 / 環境 / 微生物
Outline of Final Research Achievements

In this study, the anaerobically methane-oxidizing and acetate-oxidizing iron reducers were enriched from 15 different deep subseafloor core sediments with crystalline iron oxides as electron acceptors. The enrichment process was monitored by high-throughput sequencing. The obtained enrichment cultures were transferred to energetically favorable media including soluble Fe(III) or the other electron acceptors, resulting in the successful isolation of two species of the iron-reducing Firmicutes bacteria. This indicates that the acetate-oxidizing crystalline iron(III) reducers were responsible for the carbon flux in the deep subseafloor sediments.

Academic Significance and Societal Importance of the Research Achievements

本研究で標的とする炭素化合物「メタンおよび酢酸」と地球第4位の構成元素「鉄」は地球上最大の根源物質であるため、その代謝に関わる未知微生物群の実体解明は、地下圏の生命活動全体を紐解くことに直結する。陸域土壌からよく検出されるDeltaproteobacteria綱に属する鉄還元・酢酸酸化菌が地下深部からは取得されなかったことから、海底下では陸上圏とは異なる結晶性酸化鉄の還元機構が存在していることが強く示唆された。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (10 results)

All 2018 2017 2016 Other

All Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results,  Open Access: 4 results) Presentation (4 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results) Remarks (2 results)

  • [Journal Article] Identification of active and taxonomically diverse 1,4-dioxane degraders in a full-scale activated sludge system by high-sensitivity stable isotope probing2018

    • Author(s)
      Aoyagi Tomo、Morishita Fumiaki、Sugiyama Yutaka、Ichikawa Daisuke、Mayumi Daisuke、Kikuchi Yoshitomo、Ogata Atsushi、Muraoka Kenji、Habe Hiroshi、Hori Tomoyuki
    • Journal Title

      The ISME Journal

      Volume: 12 Issue: 10 Pages: 2376-2388

    • DOI

      10.1038/s41396-018-0201-2

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Draft genome sequence of Geobacter pelophilus Strain Dfr2, a ferric iron reducing bacterium2017

    • Author(s)
      青柳 智、小池 英明、森田 友岳、佐藤 由也、羽部 浩、堀 知行
    • Journal Title

      Genome Announcements

      Volume: 5-24 Issue: 24

    • DOI

      10.1128/genomea.00537-17

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Sulfur-oxidizing bacteria mediate microbial community succession and element cycling in launched marine sediment2017

    • Author(s)
      Ihara H, Hori T, Aoyagi T, Takasaki M, Katayama Y
    • Journal Title

      Front. Microbiol.

      Volume: 8 Pages: 152-152

    • DOI

      10.3389/fmicb.2017.00152

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Predominance of Viable Spore-Forming Piezophilic Bacteria in High-Pressure Enrichment Cultures from ~1.5 to 2.4 km-Deep Coal-Bearing Sediments below the Ocean Floor2017

    • Author(s)
      Jiasong Fang、加藤千明、Gabriella Runko、能木 裕一、堀 知行、Jiangtao Li、諸野祐樹、稲垣史生
    • Journal Title

      Frontiers in Microbiology

      Volume: 8 (137) Pages: 1-10

    • DOI

      10.3389/fmicb.2017.00137

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 結晶性酸化鉄の微生物還元とその地球炭素循環との関わり2018

    • Author(s)
      堀知行
    • Organizer
      第70回日本生物工学会大会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Ultra-high-sensitivity rRNA-SIP reveals nitrate-driven metabolic interaction of sulfur-oxidizing bacterial communities in deposited marine sediments2016

    • Author(s)
      青柳 智、Navarro R. Ronald、眞弓 大介、羽部 浩、片山葉子、高崎みつる、堀 知行
    • Organizer
      11th International Congress on Extremophiles
    • Place of Presentation
      Kyoto University、Kyoto、Japan
    • Year and Date
      2016-09-12
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Tracing interspecies carbon transfer of sulfur-oxidizing bacterial communities in deposited marine sediments by ultra-high-sensitivity stable isotope probing of rRNA2016

    • Author(s)
      青柳 智、Navarro R. Ronald、眞弓 大介、羽部 浩、片山葉子、高崎みつる、堀 知行
    • Organizer
      16th International Symposium on Microbial Ecology
    • Place of Presentation
      Palais des Congres、Montreal、Canada
    • Year and Date
      2016-08-21
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Isolation of sulfur-oxidizing bacteria playing the critical role in element cycling in tsunami sediment under aerobic conditions2016

    • Author(s)
      猪原 英之、堀 知行、高崎みつる、片山葉子
    • Organizer
      16th International Symposium on Microbial Ecology
    • Place of Presentation
      Palais des Congres、Montreal、Canada
    • Year and Date
      2016-08-21
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Remarks] 環境微生物グループHP

    • URL

      https://unit.aist.go.jp/emri/114envmicrob/index.html

    • Related Report
      2017 Annual Research Report
  • [Remarks] 産業技術総合研究所 環境微生物研究グループホームページ

    • URL

      https://unit.aist.go.jp/emri/114envmicrob/index.html

    • Related Report
      2016 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2020-03-30  

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