Identification of key molecule for influenza virus entry
Project/Area Number |
16H06227
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Research Category |
Grant-in-Aid for Young Scientists (A)
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Allocation Type | Single-year Grants |
Research Field |
Experimental pathology
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Research Institution | Hokkaido University |
Principal Investigator |
|
Project Period (FY) |
2016-04-01 – 2020-03-31
|
Project Status |
Completed (Fiscal Year 2019)
|
Budget Amount *help |
¥25,090,000 (Direct Cost: ¥19,300,000、Indirect Cost: ¥5,790,000)
Fiscal Year 2019: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2018: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2017: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2016: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
|
Keywords | ウイルス / エンドサイトーシス / シグナル伝達 / カルシウム / インフルエンザウイルス / イメージング |
Outline of Final Research Achievements |
We have discovered that the Ca2+ channel, a transmembrane protein that allows Ca2+ to move across the plasma membrane, is the key receptor molecule for influenza virus infections. Furthermore, treating human cells with a calcium channel blocker (CCB), which is commonly used as anti-hypertension drug, significantly suppressed virus infections.
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Academic Significance and Societal Importance of the Research Achievements |
我々は従前のウイルス中心のインフルエンザ研究等とは異なり、宿主細胞側シグナル伝達をターゲットとした研究から、カルシウムチャネルがインフルエンザウイルス感染に鍵となる受容体タンパク質であると同定した。インフルエンザウイルス宿主侵入機構の全貌を解明した研究成果は高い評価を受けている。さらに、我々が提案する手法はこれまでの治療標的とは全く異なるプロセスである宿主細胞への“侵入”を標的とするため、新規治療法の開発に直結する可能性を秘めている。
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Report
(5 results)
Research Products
(23 results)
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[Journal Article] Calcium Wave Promotes Cell Extrusion2020
Author(s)
Takeuchi Y、Narumi R、Akiyama R、Vitiello E、Shirai T、Tanimura N、Kuromiya K、Ishikawa S、Kajita M、Tada M、Haraoka Y、Akieda Y、 Ishitani T、Fujioka Y、Ohba Y、Yamada S、Hosokawa Y、Toyama Y、Matsui T、Fujita Y
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Journal Title
Current Biology
Volume: 30
Issue: 4
Pages: 670-681.e6
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] A Peptide Derived from Phosphoinositide 3-kinase Inhibits Endocytosis and Influenza Virus Infection2019
Author(s)
Fujioka Y, Satoh AO, Horiuchi K, Fujioka M, Tsutsumi K, Sasaki J, Nepal P, Kashiwagi S, Paudel S, Nishide S, Nanbo A, Sasaki T, Ohba Y
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Journal Title
Cell Structure and Function
Volume: 44
Issue: 1
Pages: 61-74
DOI
NAID
ISSN
0386-7196, 1347-3700
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Folding Latency of Fluorescent Proteins Affects the Mitochondrial Localization of Fusion Proteins2019
Author(s)
Sayaka Kashiwagi, Yoichiro Fujioka, Aya O. Satoh, Aiko Yoshida, Mari Fujioka, Prabha Nepal, Atsushi Tsuzuki, Ozora Aoki, Sarad Paudel, Hitoshi Sasajima, Yusuke Ohba
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Journal Title
Cell Structure and Function
Volume: 44
Issue: 2
Pages: 183-194
DOI
NAID
ISSN
0386-7196, 1347-3700
Related Report
Peer Reviewed / Open Access
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[Journal Article] Localization of BCR-ABL to Stress Granules Contributes to Its Oncogenic Function2019
Author(s)
Sayaka Kashiwagi, Yoichiro Fujioka, Takeshi Kondo, Aya O. Satoh, Aiko Yoshida, Mari Fujioka, Hitoshi Sasajima, Maho Amano, Takanori Teshima, Yusuke Ohba
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Journal Title
Cell Structure and Function
Volume: 44
Issue: 2
Pages: 195-204
DOI
NAID
ISSN
0386-7196, 1347-3700
Related Report
Peer Reviewed / Open Access
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[Journal Article] A Sialylated Voltage-Dependent Ca2+ Channel Binds Hemagglutinin and Mediates Influenza A Virus Entry into Mammalian Cells2018
Author(s)
Fujioka Y, Nishide S, Ose T, Suzuki T, Kato I, Fukuhara H, Fujioka M, Horiuchi K, Satoh AO, Nepal P, Kashiwagi S, Wang J, Horiguchi M, Sato Y, Paudel S, Nanbo A, Miyazaki T, Hasegawa H, Maenaka K, Ohba Y
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Journal Title
Cell Host Microbe
Volume: 23
Issue: 6
Pages: 809-818
DOI
NAID
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] A leukemogenic kinase, FIP1L1-PDGFRA, and a SUMO E3 ligase, PIAS1, form a positive crosstalk via their enzymatic activities.2017
Author(s)
M. Ibata, J. Iwasaki, Y. Fujioka, K. Nakagawa, S. Darmanin, M. Onozawa, D. Hashimoto, Y. Ohba, S. Hatakeyama, T. Teshima and T. Kondo.
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Journal Title
Cancer Sci.
Volume: 108
Issue: 2
Pages: 200-207
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Rab5-regulated endocytosis plays a crucial role in apical extrusion of transformed cells.2017
Author(s)
S. Saitoh, T. Maruyama, Y. Yako, M. Kajita, Y. Fujioka, Y. Ohba, N. Kasai, N. Sugama, S. Kon, S. Ishikawa, T. Hayashi, T. Yamazaki, M. Tada and Y. Fujita.
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Journal Title
Proc. Natl. Acad. Sci. U.S.A.
Volume: 114
Issue: 12
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Improved FRET Biosensor for the Measurement of BCR-ABL Activity in Chronic Myeloid Leukemia Cells2017
Author(s)
M. Horiguchi, M. Fujioka, T. Kondo, Y. Fujioka, X. Li, K. Horiuchi, A.O. Satoh, P. Nepal, S. Nishide, A. Nanbo, T. Teshima and Y. Ohba.
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Journal Title
Cell Structure and Function
Volume: 42
Issue: 1
Pages: 15-26
DOI
NAID
ISSN
0386-7196, 1347-3700
Related Report
Peer Reviewed / Open Access
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[Journal Article] Cell competition with normal epithelial cells promotes apical extrusion of transformed cells through metabolic changes2017
Author(s)
Kon S, Ishibashi K, Katoh H, Kitamoto S, Shirai T, Tanaka S, Kajita M, Yako Y, Nishikawa A, Kameda I, Sasaki Y, Enoki R, Honma S, Imamura H, Oshima M, Soga T, Miyazaki J, Duchen M R, Nam J-M, Onodera Y, Yoshioka S, Kikuta J, Ishii M, Imajo M, Nishida E, Fujioka Y, Ohba Y, Sato T, and Fujita Y
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Journal Title
Nat. Cell Biol.
Volume: 印刷中
Related Report
Peer Reviewed
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[Presentation] Influenza viruses are internalized into host cells via calcium signaling-mediated endocytosis2017
Author(s)
Fujioka Y, Nishide S, Ose T, Kato I, Fukuhara H, Fujioka M, Horiuchi K, Satoh A, Nepal P, Kashiwagi S, Wang J, Horiguchi M, Paudel S, Nanbo A, Miyazaki T, Maenaka K, Ohba Y
Organizer
3rd International symposium on cell competition
Related Report
Int'l Joint Research
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