| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
The purpose of this study was to investigate factors that are necessary to develop high-grade endometrial cancer (EMC). We focused PTEN and p53 gene mutations which are frequently altered in EMC and estrogen which is thought to relate to EMC progression. Using mice with endometrial deletion of Pten, we found that the invasion of tumor cells into the myometrial layer was promoted by the supplement of physiologically low level of estrogen. By generating mice carried uterine epithelial-specific loss of p53 or missense mutation of p53, we found these mutations are not enough to develop high-grade EMC. We then tried to introduce other genes directly to uterine epithelium by novel in vivo genetic engineering method; however, we could not analyze the effects further because the gene induction was temporary. We think to reveal the gene mutations and/or overexpression for developing high-grade EMC by improving in vivo genetic engineering method.
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