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Elucidation of the food chain for conservation of Tsushima leopard cat, and relation analysis between gut microbiome and individual characteristics

Research Project

Project/Area Number 16H06892
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Conservation of biological resources
Research InstitutionKyoto University

Principal Investigator

Ito Hideyuki  京都大学, 野生動物研究センター, 特任研究員 (10779648)

Co-Investigator(Renkei-kenkyūsha) MURAYAMA Miho  京都大学, 野生動物研究センター, 教授 (60293552)
Research Collaborator SATO Hiroki  環境省・九州地方環境事務所, 対馬自然保護官事務所(対馬野生生物保護センター), 首席自然保護官
MINOURA Chisa  環境省・九州地方環境事務所, 対馬自然保護官事務所(対馬野生生物保護センター), 獣医師
TAKAGI Naoko  京都市動物園, 種の保存展示課, 飼育員
YASUI Saki  京都市動物園, 種の保存展示課, 飼育員
Project Period (FY) 2016-08-26 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsツシマヤマネコ / 食性解析 / 腸内細菌叢 / 次世代シークエンス / 遺伝的多様性 / 食性 / マイクロサテライト / メタゲノム / 細菌叢解析
Outline of Final Research Achievements

Tsushima leopard cat inhabits only in Tsushima island, Nagasaki Prefecture. Due to various factors (eg. destruction of habitats), the number of population is decreasing.
Analysis using feces which is a noninvasive technique is very useful for analysis of wild animals. Accurate information on food is extremely useful for in situ and ex situ conservation and captive breeding management. Analysis of gut microbiome which is related to animal health (eg. immunity and fat) can be useful information for conservation. In this study, we analyzed food and metagenome analysis of gut microbiome using the next generation sequencer.
DNA was extracted using feaces from captive individuals and wild individuals, and PCR and tag labeling were conducted for the purpose of preparing a library for the next generation sequence, but the concentration of the final library was low and the conditions had to be reexamined.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • Research Products

    (5 results)

All 2017

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (4 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Contrasting results from molecular and pedigree-based population diversity measures in captive zebra highlight challenges facing genetic management of zoo populations.2017

    • Author(s)
      Ito H, Ogden R, Langenhorst T, Inoue-Murayama M
    • Journal Title

      Zoo Biology

      Volume: 36 Issue: 1 Pages: 87-94

    • DOI

      10.1002/zoo.21342

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] 京都大学野生動物研究センターにおけるGenetic resource bankの概要及び応用例2017

    • Author(s)
      伊藤英之, 佐藤悠, 小林宏美, 森村成樹, 大沼学, 村山美穂
    • Organizer
      第23回日本野生動物医学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Genetic diversities in the two captive grevy’s zebra populations2017

    • Author(s)
      Hideyuki ITO
    • Organizer
      The 6th International Seminar on Biodiversity and Evolution
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Genetic polymorphisms of candidate genes related to behavior traits in zebra species2017

    • Author(s)
      Hideyuki Ito, Rob Ogden, Tanya Langenhorst, Miho Murayama-Inoue
    • Organizer
      11th International Conference on Behaviour, Physiology and Genetics of Wildlife
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 京都大学野生動物研究センター(WRC)の性判別研修会について2017

    • Author(s)
      村山美穂・伊藤英之・森村成樹
    • Organizer
      動物園大学7
    • Place of Presentation
      愛媛県立とべ動物園(愛媛県伊予郡砥部町)
    • Related Report
      2016 Annual Research Report

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Published: 2016-09-02   Modified: 2019-03-29  

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