Comprehensive analysis of RNA binding proteins in the immune system
Project/Area Number |
16H06898
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Research Category |
Grant-in-Aid for Research Activity Start-up
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Allocation Type | Single-year Grants |
Research Field |
Immunology
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Research Institution | Kyoto University |
Principal Investigator |
Uehata Takuya 京都大学, ウイルス・再生医科学研究所, 助教 (50785970)
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Project Period (FY) |
2016-08-26 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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Keywords | RNA結合蛋白質 / 転写後制御 / 炎症制御 / RNA結合タンパク質 / RNA結合タンパク / RBPome / 免疫細胞 / プロテオーム / 免疫学 |
Outline of Final Research Achievements |
RNA binding proteins (RBPs) play a central role in the fate decision of mRNA. Better understanding of dynamic changes in RBP-RNA interaction during inflammation is important for discovery of new post-transcriptional mechanisms. In this study, RBP-RNA interactome was determined using T cell line post-activation. I found that 750 RBPs were detected and that a gene cluster related to inflammatory responses was identified from among them. RBPs that are presumably involved in inflammatory responses were selected and I obtained preliminary data that these RBPs regulate inflammation including cytokine production. Collectively, I demonstrated that RBPs underwent a dynamic change in RNA interaction status during inflammation.
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Report
(3 results)
Research Products
(4 results)