The mechanism of TMJ-OA pathogenesis in HIF-1a gene deficient mice
| Project/Area Number |
16H07019
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| Research Category |
Grant-in-Aid for Research Activity start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthodontics/Pediatric dentistry
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| Research Institution | The University of Tokushima |
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Principal Investigator |
MORI Hiroki 徳島大学, 大学院医歯薬学研究部(歯学系), 助教 (90779985)
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| Project Period (FY) |
2016-08-26 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | TMJ / 歯学 / 低酸素応答遺伝子 / 顎関節 / 病理学 |
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| Outline of Final Research Achievements |
Mechanical stress was applied to the TMJ of C57BL/6NCr wild-type (WT) and HIF-1α+/－ mice with a sliding plate for 10 days. Histological analysis was performed by HE staining, Safranin-O/Fast green staining, and immunostaining specific for articular cartilage homeostasis.HIF-1α+/－ mice had thinner cartilage and smaller areas of proteoglycan than WT controls, without and with mechanical stress. Mechanical stress resulted in prominent degenerative changes with increased expression of HIF-1α, VEGF, and the apoptosis factor cleaved Caspase-3 in condylar cartilage. Our results indicate that HIF-1α may be important for articular cartilage homeostasis and protective against articular cartilage degradation in the TMJ under mechanical stress condition, therefore HIF-1α could be an important new therapeutic target in TMJ-OA.
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Report
(3 results)
Research Products
(1 results)
