| Project/Area Number |
16K06870
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Biofunction/Bioprocess
|
|---|
| Research Institution | Nagaoka University of Technology |
|---|
Principal Investigator |
Shida Yosuke 長岡技術科学大学, 工学研究科, 助教 (70573880)
|
|---|
| Research Collaborator |
YAOI katsuro
KIDOKORO syunichi
OKU naoya
|
|---|
| Project Period (FY) |
2016-04-01 – 2019-03-31
|
| Project Status |
Completed (Fiscal Year 2018)
|
| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
|
|---|
| Keywords | 誘導発現メカニズム / Trichoderma reesei / β-グルコシダーゼ / セルラーゼ / 誘導発現 / トリコデルマ / β－グルコシダーゼ / 酵素 / 発現制御 / 蛋白質 / シグナル伝達 / 応用微生物 |
|---|
| Outline of Final Research Achievements |
In the filamentous fungus Trichodrema reesei, the amino acid mutation at position 409 of intracellular β-glucosidase BGLII leads to enhanced production of cellulase. In order to clarify the relationship between BGLII mutation and cellulase production, saturation mutagenesis was introduced at the mutation site and its influence on cellulase production of T. reesei was analyzed. As a result, the mutation that gave the highest cellulase productivity was the V409T mutation. Analysis of mutant BGLII by heterologous expression revealed that the V409T mutation improves the hydrolysis activity more than twice that of the wild strain and enhances the transglycosylation activity to generate oligosaccharides. Moreover, its transglycosylation products were suggested that they are the inducers of cellulase production.
|
| Academic Significance and Societal Importance of the Research Achievements |
糸状菌Trichoderma reeseiは植物バイオマス分解酵素（セルラーゼ）を大量に生産するため、非常に有用な菌株である。本菌がセルラーゼを生産するためには誘導物質が必要であるが、その誘導物質生成の一端を担っている菌体内酵素β-グルコシダーゼの変異がセルラーゼ生産に与える影響を解析し、409番目のアミノ酸をバリンからスレオニンに変異させることが最も高いセルラーゼ生産性をもたらした。本研究の成果は、本菌の工業利用を促進するとともに、β-グルコシダーゼの酵素機能を理解する上で大きな意義がある。
|
