| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Here, we attempted to develop avian genome manipulation technology for the generation of transgenic avian bioreactor. We designed various guide RNAs against egg white-related genes and evaluated genome editing efficiency on chicken cell genome of CEF and DF-1 cells. After constructing knock-in vector encoding reporter genes, transgene could be integrated correctly into expected locus using CRISPR/Cas9 system. We decided the condition that endogenous OVA locus was activated by dCas9 transactivation system. We optimized gene transfer condition into chicken embryos to observe the expression of transgene.
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