Efforts toward precise and efficient homologous recombination in genome editing.

• Project/Area Number: 16K07093
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: Kumamoto University
• Principal Investigator: Araki Kimi 熊本大学, 生命資源研究・支援センター, 教授 (90211705)
• Co-Investigator(Kenkyū-buntansha): 荒木 正健 熊本大学, 生命資源研究・支援センター, 准教授 (80271609)
• Research Collaborator: TAKEDA Naoki
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2017: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: ゲノム編集 / 相同組換え / ES細胞 / マウスES細胞 / 相同組み換え / 遺伝子改変マウス / 発生工学 / 遺伝子操作

Outline of Final Research Achievements

Genome editing using CRISPR / Cas9 is an essential tool for producing genetically modified mice. However, when double strand breaks (DSBs) are introduced on the genome DNA to induce homologous recombination (HR)in mouse ES cells, although the rate of successful HR is dramatically increased, alleles in which HR have not occurred frequently show gross rearrangement. We developed a system that can distinguish between accurate gene targeting by HR and gene targeting with rearrangement in ES cells. We examined the use of Cas9 D10A nickase, which rarely induce rearrangement, and found that multiple nicks on the same strand is effective, although the efficient of HR was lower than that of DSB.

Academic Significance and Societal Importance of the Research Achievements

ゲノム編集は、DNAを目的の場所で2本鎖切断(DSB)することで遺伝子の改変を効率よく行う技術で、遺伝子改変マウス作製効率は飛躍的に上昇した。しかし、DSBを加えると、２つある対立アレルのうち、目的通りの遺伝子改変が起こらなかった対立アレルでは、大きなリアレンジが高い頻度で生じてしまう。そのような細胞やマウスは解析に適さないため、リアレンジを抑える技術の開発は解析効率を上げるために重要である。１本鎖切断を起こすCas9 D10A nickaseを効果的に利用できることがわかったので、マウスES細胞を用いた遺伝子改変を安全かつ迅速に進めることができるようになった。

Research Products

• [Journal Article] Loss of Endogenous HMGB2 Promotes Cardiac Dysfunction and Pressure Overload-Induced Heart Failure in Mice (2019)
  • Author(s): Sato M, Miyata K, Tian Z, Kadomatsu T, Ujihara Y, Morinaga J, Horiguchi H, Endo M, Zhao J, Zhu S, Sugizaki T, Igata K, Muramatsu M, Minami T, Ito T, Bianchi ME, Mohri S, Araki K, Node K, Oike Y
  • Journal Title: Circulation Journal Volume: 83 Issue: 2 Pages: 368-378
  • DOI: 10.1253/circj.CJ-18-0925
  • NAID: 130007557092
  • ISSN: 1346-9843, 1347-4820
  • Year and Date: 2019-01-25
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] LPA5 signaling is involved in multiple sclerosis-mediated neuropathic pain in the cuprizone mouse model (2018)
  • Author(s): Tsukahara Ryoko、Yamamoto Shinji、Yoshikawa Keisuke、Gotoh Mari、Tsukahara Tamotsu、Neyama Hiroyuki、Ishii Satoshi、Akahoshi Noriyuki、Yanagida Keisuke、Sumida Hayakazu、Araki Masatake、Araki Kimi、Yamamura Ken-ichi、Murakami-Murofushi Kimiko、Ueda Hiroshi
  • Journal Title: Journal of Pharmacological Sciences Volume: 136 Issue: 2 Pages: 93-96
  • DOI: 10.1016/j.jphs.2018.01.001
  • Peer Reviewed / Open Access
• [Journal Article] Amyloid deposition in a mouse model humanized at the transthyretin and retinol-binding protein 4 loci (2018)
  • Author(s): Li Xiangshun、Lyu Yanyi、Shen Jingling、Mu Yanshuang、Qiang Lixia、Liu Li、Araki Kimi、Imbimbo Bruno P.、Yamamura Ken-ichi、Jin Shoude、Li Zhenghua
  • Journal Title: Laboratory Investigation Volume: 98 Issue: 4 Pages: 512-524
  • DOI: 10.1038/s41374-017-0019-y
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Differential roles of Rad18 and Chk2 in genome maintenance and skin carcinogenesis following UV exposure. (2018)
  • Author(s): Tanoue, Y., Toyoda, T., Sun, J, Mustofa, M.K., Tateishi, C., Endo, S., Motoyama, N., Araki, K., Wu, D., Okuno, Y., Tsukamoto, T., Takeya, M., Ihn, H., Vaziri, C., Tateishi, S.
  • Journal Title: J. Invest. Dermatol. Volume: 138 Issue: 12 Pages: 2550-2557
  • DOI: 10.1016/j.jid.2018.05.015
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Impact of loss-of-function mutations at the RNF43 locus on colorectal cancer development and progression (2018)
  • Author(s): Eto T, Miyake K, Nosho K, Ohmuraya M, Imamura Y, Arima K, Kanno S, Fu L, Kiyozumi Y, Izumi D, Sugihara H, Hiyoshi Y, Miyamoto Y, Sawayama H, Iwatsuki M, Baba Y, Yoshida N, Furukawa T, Araki K, Baba H, Ishimoto T
  • Journal Title: The Journal of Pathology Volume: 245 Issue: 4 Pages: 445-455
  • DOI: 10.1002/path.5098
  • Peer Reviewed
• [Journal Article] Differential expression of Lutheran/BCAM regulates biliary tissue remodeling in ductular reaction during liver regeneration (2018)
  • Author(s): Miura Yasushi、Matsui Satoshi、Miyata Naoko、Harada Kenichi、Kikkawa Yamato、Ohmuraya Masaki、Araki Kimi、Tsurusaki Shinya、Okochi Hitoshi、Goda Nobuhito、Miyajima Atsushi、Tanaka Minoru
  • Journal Title: eLife Volume: 7 Pages: 36572-36572
  • DOI: 10.7554/elife.36572
  • Peer Reviewed / Open Access
• [Journal Article] Inhibition of 15-PGDH causes Kras-driven tumor expansion through prostaglandin E2-ALDH1 signaling in the pancreas (2018)
  • Author(s): Arima Kota、Ohmuraya Masaki、Miyake Keisuke、Koiwa Mayu、Uchihara Tomoyuki、Izumi Daisuke、Gao Feng、Yonemura Atsuko、Bu Luke、Okabe Hirohisa、Imai Katsunori、Hashimoto Daisuke、Baba Yoshifumi、Chikamoto Akira、Yamashita Yo-ichi、Furukawa Toru、Araki Kimi、Baba Hideo、Ishimoto Takatsugu
  • Journal Title: Oncogene Volume: 38 Issue: 8 Pages: 1211-1224
  • DOI: 10.1038/s41388-018-0510-y
  • Peer Reviewed
• [Journal Article] Generation of and characterization of anti-IL-11 antibodies using newly established Il11-deficient mice. (2018)
  • Author(s): Deguchi Y, Nishina T, Asano K, Ohmuraya M, Nakagawa Y, Nakagata N, Sakuma T, Yamamoto T, Araki K, Mikami T, Tanaka M, Nakano H.
  • Journal Title: Biochemical Biophysical Research Communications Volume: 505 Issue: 2 Pages: 453-459
  • DOI: 10.1016/j.bbrc.2018.09.128
  • Peer Reviewed / Open Access
• [Journal Article] Emergence of immunoregulatory Ym1+Ly6Chi monocytes during recovery phase of tissue injury (2018)
  • Author(s): Ikeda Naoki、Asano Kenichi、Kikuchi Kenta、Uchida Yoshimi、Ikegami Hiroki、Takagi Ryo、Yotsumoto Satoshi、Shibuya Takumi、Makino-Okamura Chieko、Fukuyama Hidehiro、Watanabe Takashi、Ohmuraya Masaki、Araki Kimi、Nishitai Gen、Tanaka Masato
  • Journal Title: Science Immunology Volume: 3 Issue: 28
  • DOI: 10.1126/sciimmunol.aat0207
  • Peer Reviewed
• [Journal Article] Deletion of GIRK2 subunit containing GIRK channels of neurons expressing dopamine transporter decrease immobility time on forced swimming in mice (2018)
  • Author(s): Honda Ikutaro、Araki Kimi、Honda Sokichi、Soeda Fumio、Shin Min-Chul、Misumi Shogo、Yamamura Ken-ichi、Takahama Kazuo
  • Journal Title: Neuroscience Letters Volume: 665 Pages: 140-146
  • DOI: 10.1016/j.neulet.2017.11.028
  • Peer Reviewed
• [Journal Article] Rescue of retinal morphology and function in a humanized mouse at the mouse retinol-binding protein locus. (2017)
  • Author(s): Liu, L., Suzuki, T., Shen, J., Wakana, S., Araki, K., Yamamura, K., Lei, L. and Li, Z.
  • Journal Title: Lab. Invest. Volume: 97 Issue: 4 Pages: 395-408
  • DOI: 10.1038/labinvest.2016.156
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] ANGPTL2 expression in intestinal stem cell niche controls epithelial regeneration and homeostasis. (2017)
  • Author(s): Horiguchi H, Endo M, Kawane K, Kadomatsu T, Terada K, Morinaga J, Araki K, Miyata K, Oike Y,
  • Journal Title: EMBO Journal Volume: 36 Issue: 4 Pages: 409-424
  • DOI: 10.15252/embj.201695690
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Soluble IL-6R expressed by myeloid cells reduces tumor-specific Th1 differentation and drives tumor progression (2017)
  • Author(s): Tsukamoto H, Fujieda K, Hirayama M, Ikeda T, Yuno A, Matsumura K, Fukuma D, Araki K, Mizuta H, Nakayama H, Senju S, Nishimura Y
  • Journal Title: Cancer Research Volume: 14 Issue: 9 Pages: 2279-2291
  • DOI: 10.1158/0008-5472.can-16-2446
  • Peer Reviewed / Open Access
• [Journal Article] Development of an efficient screening system to identify novel bone metabolism-related genes using the exchangeable gene trap mutagenesis mouse models (2017)
  • Author(s): Syuji Kurogi, Tomohisa Sekimoto, Taro Funamoto, Tomomi Ohta, Shihoko Nakamura, Takuya Nagai, Mai Nakahara, Kumiko Yoshinobu, Kimi Araki, Masatake Araki, Etsuo Chosa
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 40692-40692
  • DOI: 10.1038/srep40692
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] MiR-142 Is Required for Staphylococcus aureus Clearance at Skin Wound Sites via Small GTPase-Mediated Regulation of the Neutrophil Actin Cytoskeleton (2017)
  • Author(s): Tanaka K, Kim SE, Yano H, Matsumoto G, Ohuchida R, Ishikura Y, Araki M, Araki K, Park S, Komatsu T, Hayashi H, Ikematsu K, Tanaka K, Hirano A, Martin P, Shimokawa I, Mori R
  • Journal Title: J Invest Dermatol Volume: 137 Issue: 4 Pages: 931-940
  • DOI: 10.1016/j.jid.2016.11.018
  • NAID: 40021436657
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Endothelial CD99 supports arrest of mouse neutrophils in venules and binds to neutrophil PILRs (2017)
  • Author(s): Goswami Debashree、Marz Sigrid、Li Yu-Tung、Artz Annette、Schafer Kerstin、Seelige Ruth、Pacheco-Blanco Mariana、Jing Ding、Bixel Maria Gabriele、Araki Masatake、Araki Kimi、Yamamura Ken-Ichi、Vestweber Dietmar
  • Journal Title: Blood Volume: 129 Issue: 13 Pages: 1811-1822
  • DOI: 10.1182/blood-2016-08-733394
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Accumulation of HLA-DR4 in Colonic Epithelial Cells Causes Severe Colitis in Homozygous HLA-DR4 Transgenic Mice (2017)
  • Author(s): Irie Atsushi、Imamura Takahisa、Michibata Yayoi、Kubo Ttatsuko、Takeda Naoki、Shibuya Isao、Sogo Shinji、Araki Kimi、Nishimura Yasuharu
  • Journal Title: Inflammatory Bowel Diseases Volume: 23 Issue: 12 Pages: 2121-2133
  • DOI: 10.1097/mib.0000000000001282
  • Peer Reviewed / Open Access
• [Journal Article] WDR11‐mediated Hedgehog signalling defects underlie a new ciliopathy related to Kallmann syndrome (2017)
  • Author(s): Kim Yeon‐Joo、Osborn Daniel PS、Lee Ji‐Young、Araki Masatake、Araki Kimi、Mohun Timothy、K?ns?koski Johanna、Brandstack Nina、Kim Hyun‐Taek、Miralles Francesc、Kim Cheol‐Hee、Brown Nigel A、Kim Hyung‐Goo、Martinez‐Barbera Juan Pedro、Ataliotis Paris、Raivio Taneli、Layman Lawrence C、Kim Soo‐Hyun
  • Journal Title: EMBO reports Volume: 19 Issue: 2 Pages: 269-289
  • DOI: 10.15252/embr.201744632
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Severe ocular phenotypes in Rbp4-deficient mice in the C57BL/6 genetic background. (2016)
  • Author(s): Shen, J., Shi, D., Suzuki, T., Xia, Z., Zhang, H., Araki, K., Wakana, S., Takeda, N., Yamamura, K., Jin, S. and Li. Z.
  • Journal Title: Lab. Invest. Volume: in press Issue: 6 Pages: 1-12
  • DOI: 10.1038/labinvest.2016.39
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] The importance of basonuclin 2 in adult mice and its relation to basonuclin 1. (2016)
  • Author(s): Vanhoutteghem A, Delhomme B, Hervé F, Nondier I, Petit JM, Araki M, Araki K, Djian P.
  • Journal Title: Mechanisms of Development Volume: Feb 29 Pages: 1-21
  • DOI: 10.1016/j.mod.2016.02.002
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Rad18 confers hematopoietic progenitor cell DNA damage tolerance independently of the Fanconi Anemia pathway in vivo. (2016)
  • Author(s): Yang, Y., Poe, J. C., Yang, L., Fedoriw, A., Desai, S., Magnuson, T., Li, Z., Fedoriw, Y., Araki, K., Gao, Y., Tateishi, S., Sarantopoulos, S. and Vaziri, C.
  • Journal Title: Nucleic Acids Res. Volume: February 15 Issue: 9 Pages: 1-15
  • DOI: 10.1093/nar/gkw072
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Cathepsin D in pancreatic acinar cells is implicated in cathepsin B and L degradation, but not in autophagic activity. (2016)
  • Author(s): Mehanna, S, Suzuki, C., Shibata, M., Sunabori, T., Imanaka, T., Araki, K., Yamamura, K., Uchiyama, Y. and Ohmuraya, M.
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 469 Issue: 3 Pages: 405-411
  • DOI: 10.1016/j.bbrc.2015.12.002
  • Peer Reviewed
• [Journal Article] Mutation of the key residue for extraribosomal function of ribosomal protein S19 cause increased grooming behaviors in mice. (2016)
  • Author(s): Jun Chen, Taku Kaitsuka, Rika Fujino, Kimi Araki, Kazuhito Tomizawa, Tetsuro Yamamoto
  • Journal Title: Neuroscience letters Volume: 629 Pages: 221-226
  • DOI: 10.1016/j.neulet.2016.07.022
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Two transgenic mouse models for beta subunit components of succinate-CoA ligase yielding pleiotropic metabolic alterations. (2016)
  • Author(s): Kacso, G., Ravasz, D., Doczi, J., Nemeth, B., Madgar, O., Saada, A., Ilin, P., Miller, C., Ostergaard, E., Iordanov, I., Adams, D., Vargedo, Z., Araki, M., Araki, K., Nakahara, M., Ito, H., Gal, A., Molnar, M., Nagy, Z., Patocs, A., Adam-Vizi, V., and Chinopoulos C.
  • Journal Title: Biochemical J. Volume: 473 Pages: 3463-3485
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Upregulation of ANGPTL6 in mouse keratinocytes enhances susceptibility to psoriasis. (2016)
  • Author(s): Tanigawa H, Miyata K, Tian Z, Aoi J, Kadomatsu T, Fukushima S, Ogata A, Takeda N, Zhao J, Zhu S, Terada K, Endo M, Morinaga J, Sugizaki T, Sato M, Morioka MS, Manabe I, Mashimo Y, Hata A, Taketomi Y, Yamamoto K, Murakami M, Araki K, Jinnin M, Ihn H, Oike Y
  • Journal Title: Scientific Reports Volume: 6 Issue: 1 Pages: 34690-34690
  • DOI: 10.1038/srep34690
  • NAID: 120006534970
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Protein kinase D regulates positive selection of CD4+ thymocytes through phosphorylation of SHP-1 (2016)
  • Author(s): Ishikawa E, Kosako H, Yasuda T, Ohmuraya M, Araki K, Kurosaki T, Saito T, Yamasaki S
  • Journal Title: Nat Commun Volume: 7 Issue: 1 Pages: 12756-12756
  • DOI: 10.1038/ncomms12756
  • NAID: 120006535062
  • Peer Reviewed / Open Access
• [Journal Article] Mtu1-mediated thiouridine formation of mitochondrial tRNAs is required for mitochondrial translation and is involved in reversible infantile liver injury (2016)
  • Author(s): Wu, Y., Wei, F. Y., Kawarada, L., Suzuki, T., Araki, K., Komohara, Y., Fujimura, A., Kaitsuka, T., Takeya, M., Oike, Y., Suzuki, T., Tomizawa, K.
  • Journal Title: PLoS genetics Volume: 12 Issue: 9 Pages: e1006355-e1006355
  • DOI: 10.1371/journal.pgen.1006355
  • Peer Reviewed / Open Access
• [Journal Article] Novel method to rescue a lethal phenotype through integration of target gene onto the X-chromosome (2016)
  • Author(s): Sakata, K. Araki, K. Nakano, H. Nishina, T. Komazawa-Sakon, S. Murai, S. Lee, G. E. Hashimoto, D. Suzuki, C. Uchiyama, Y. Notohara, K. Gukovskaya, A. S. Gukovsky, I. Yamamura, K. I. Baba, H. Ohmuraya, M.
  • Journal Title: Sci Rep Volume: 6 Issue: 1 Pages: 37200-37200
  • DOI: 10.1038/srep37200
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Short form FLICE-inhibitory protein promotes TNFalpha-induced necroptosis in fibroblasts derived from CFLARs transgenic mice (2016)
  • Author(s): Shindo, R. Yamazaki, S. Ohmuraya, M. Araki, K. Nakano, H.
  • Journal Title: Biochem Biophys Res Commun Volume: 480 Issue: 1 Pages: 23-28
  • DOI: 10.1016/j.bbrc.2016.10.015
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] c-Maf過剰発現によって生じるT細胞分化異常の原因の究明 (2019)
  • Author(s): 昇地高雅、入江厚、高橋智、荒木喜美
  • Organizer: 第１回日本遺伝学会春季分科会
• [Presentation] Role of VPS52 During Early Mouse Delopment. (2019)
  • Author(s): Michihiko SUGIMOTO, Kimi ARAKI
  • Organizer: 2019 AMMRA & AMPC Meeting
  • Int'l Joint Research
• [Presentation] Motor protein myosinx is a novel regulatory factor for longitudinal bone growth and trabecular bone microstructures via the normal endochondral ossification in the growth plate. (2019)
  • Author(s): Taro Kataoka, Masaru Tamura, Akiteru Maeno, Toshihiko Shiroishi, Kimi Araki
  • Organizer: 2019 AMMRA & AMPC Meeting
  • Int'l Joint Research
• [Presentation] 可変型遺伝子トラップ法を用いた骨に影響を及ぼす新規遺伝子群の網羅的機能解析 (2018)
  • Author(s): 関本朝久、黒木修司、舩元太郎、永井琢哉、田島卓也、谷口昇、中原舞、吉信公美子、荒木喜美、荒木正健、帖佐悦男
  • Organizer: 第91回日本整形外科学会学術総会
• [Presentation] 骨表現型スクリーニングで選別したTmem161a欠損トラップマウスは明らかな骨量増加を呈する (2018)
  • Author(s): 永井琢哉、関本朝久、黒木修司、舩元太郎、田島卓也、谷口昇、中原舞、吉信公美子、荒木喜美、荒木正健、帖佐悦男
  • Organizer: 第91回日本整形外科学会学術総会
• [Presentation] 生体内におけるLincRNA-p21の機能解析 (2018)
  • Author(s): 古畑理樹、今坂 舞、藤川 遥平、荒木 正健、吉信 公美子、荒木 喜美
  • Organizer: 先端モデル動物支援プラットフォーム 若手支援技術講習会
• [Presentation] 遺伝子改変マウスを使って研究する時、手を抜いてはいけないこと (2018)
  • Author(s): 荒木喜美
  • Organizer: 先端モデル動物支援プラットフォーム 若手支援技術講習会
• [Presentation] コンディショナルノックアウトを効率良く行うためのタモキシフェン投与法の検討 (2018)
  • Author(s): 吉信公美子、荒木美幸、森田彩香、國場訓、荒木喜美、荒木正健
  • Organizer: 日本遺伝学会第90回大会
• [Presentation] 海綿骨構造と身長を制御するモータータンパク質Myosin10の機能解析 (2018)
  • Author(s): 片岡太郎、田村勝、前野哲輝、城石俊彦、荒木喜美
  • Organizer: 日本遺伝学会第90回大会
• [Presentation] Identification of responsible genes for Stmm 1 a locus conferring resistance to early-stage chemically induced skin tumors (2018)
  • Author(s): Kazuhiro Okumura, Megumi Saito, Eriko Isogai, Kimi Araki, Yuichi Wakabayashi
  • Organizer: 第77回日本癌学会学術総会
• [Presentation] The genetic polymorphism in p19Arf confersresistance to tumor progression (2018)
  • Author(s): Megumi Saito, Kazuhiro Okumura, Eriko Isogai, Kimi Araki, Yuichi Wakabayashi
  • Organizer: 第77回日本癌学会学術総会
• [Presentation] 可変型遺伝子トラップ法を用いた新規骨代謝関連遺伝子群の網羅的機能解析 (2018)
  • Author(s): 山口洋一朗 関本朝久 永井琢哉 舩元太郎 田島卓也 谷口昇 今坂舞 荒木喜美 吉信公美子 荒木正健 帖佐悦男
  • Organizer: 第33回日本整形外科学会基礎学術集会
• [Presentation] プロタミン2遺伝子改変マウスの作製と解析 (2018)
  • Author(s): 竹田直樹、小林千余子、荒木喜美
  • Organizer: 第41回日本分子生物学会年会
• [Presentation] マウスゲノムにおける遺伝子および転写産物の存在しない領域に集積するトラップクローンの探索 (2018)
  • Author(s): 齋藤 桂花、堤 成美、原田 実穂、吉信 公美子、荒木 喜美、荒木 正健
  • Organizer: 第41回日本分子生物学会年会
• [Presentation] Analyses of Tmem161a function in bone metabolism using the exchangeable gene trap mutagenesis show significant bone ingrowth. (2018)
  • Author(s): Nagai T, Sekimoto T, Kurogi S, Funamoto T, Tajima T, Imasaka M, Yoshinobu K, Araki K, Araki M, Chosa E
  • Organizer: Australian New Zealand Bone & Mineral Society Annual Scientific Meeting 2018
  • Int'l Joint Research
• [Presentation] Generation of genome edited mice by electroporation with high efficiency and high survival rate. (2018)
  • Author(s): Michihiko SUGIMOTO, Daisuke ARIYASU, Kei-ichiro ISHIGURO, Shingo ITO, Kimi ARAKI.
  • Organizer: 平成29年度先端モデル動物支援プラットフォーム「成果発表会」
• [Presentation] 染色体特異的にクラスターを形成しているトラップ領域（CSCT）の解析 (2017)
  • Author(s): 荒木 正健、武田 伊世、大賀 敏範、江頭 聡、中原 舞、吉信 公美子、荒木 喜美
  • Organizer: 第64回日本実験動物学会総会
• [Presentation] An easy and high efficient technique for mouse genome manipulation by electroporation (2017)
  • Author(s): Michihiko Sugimoto, Daisuke Ariyasu, Kei-ichiro Ishiguro, Shingo Ito, Kimi Araki.
  • Organizer: AMMRA & AMPC meeting
  • Int'l Joint Research
• [Presentation] 遺伝子改変マウス作製の落とし穴 ~落ちて後悔しないために~ (2017)
  • Author(s): 荒木 喜美
  • Organizer: 平成29年度先端モデル動物支援プラットフォーム若手支援技術講習会
• [Presentation] エレクトロポレーションを用いた簡便・高効率マウスゲノム操作技術 (2017)
  • Author(s): 杉本道彦、有安大典、石黒啓一郎、伊藤慎悟、荒木喜美
  • Organizer: 日本遺伝学会第89回大会
• [Presentation] 染色体特異的にクラスターを形成しているトラップ領域：CSCT13は、マウス初期発生過程において重要な役割を演じていることが示唆された (2017)
  • Author(s): 荒木 正健、武田 伊世、中原 舞、吉信 公美子、 荒木 喜美
  • Organizer: 日本遺伝学会第89回大会
• [Presentation] 常染色体優性遺伝性GH1遺伝子異常症モデルマウスのGH分泌不全は、Ghrhr mRNAの低下による (2017)
  • Author(s): 有安 大典、久保 英美香、芝田 晋介、長谷川 行洋、長谷川 奉延、荒木 喜美
  • Organizer: 第51回日本小児内分泌学会学術集会
• [Presentation] 可変型遺伝子トラップクローンデータベース『EGTC』の開発及び解析 (2017)
  • Author(s): 荒木 正健、吉信 公美子、中原 舞、山村 研一、荒木 喜美
  • Organizer: トーゴーの日シンポジウム
• [Presentation] エレクトロポレーション法によるCRISPR/Cas9ゲノム編集マウス作製時における高効率化と生存率向上の試み (2017)
  • Author(s): 杉本道彦、有安大典、石黒啓一郎、伊藤慎悟、荒木喜美
  • Organizer: 第40回日本分子生物学会年会
• [Presentation] 生体内におけるLincRNA-p21の発現解析 (2017)
  • Author(s): 古畑 理樹, 今坂 舞, 伊東 春香, 荒木 正健, 吉信 公美子, 山村 研一, 荒木 喜美
  • Organizer: 第40回日本分子生物学会年会
• [Presentation] 常染色体優性遺伝性GH1遺伝子異常症の発症機序に関する検討 ー遺伝子置換システムを用いたヒト化GHマウスの作出と解析ー (2017)
  • Author(s): 久保 英実香、有安 大典、芝田 晋介、荒木 喜美
  • Organizer: 第40回日本分子生物学会年会
• [Presentation] 哺乳類ミトコンドリアtRNAに存在する5-メチルシチジンの機能解析 (2016)
  • Author(s): 北川 翔，中野 沙緒里，鈴木 健夫，荒木 喜美，荒木 正健，鈴木 勉
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: 神奈川県横浜市（パシフィコ横浜）
  • Year and Date: 2016-11-30
• [Presentation] p53により誘導されるLincRNA-p21の生体内発現解析 (2016)
  • Author(s): 古畑 理樹，中原 舞，伊東 春香，木戸 悠次郎，大村谷 昌樹，荒木 正健，吉信 公美子，山村 研一，荒木 喜美
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: 神奈川県横浜市（パシフィコ横浜）
  • Year and Date: 2016-11-30
• [Presentation] Gene-trap mutagenesis is useful for analysis of long intergenic non-coding RNA genes in vivo. (2016)
  • Author(s): (14).Mai, Nakahara., Kumiko, Yoshinobu., Riki, Furuhata., Yujiro, Kido., Michihiko, Sugimoto., Ken-ichi, Yamamura., Masatake, Araki., and Kimi, Araki.
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: 神奈川県横浜市（パシフィコ横浜）
  • Year and Date: 2016-11-30
• [Presentation] 常染色体優性遺伝性GH1遺伝子異常症の発症機序に関する検討－遺伝子置換システムを用いたヒト化GHマウスの作出－ (2016)
  • Author(s): 久保 英実香，有安 大典，荒木 喜美
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: 神奈川県横浜市（パシフィコ横浜）
  • Year and Date: 2016-11-30
• [Presentation] 常染色体優性遺伝性GH1遺伝子異常症の発症機序に関する検討－ヒト化GHマウスを用いたin vivo解析－ (2016)
  • Author(s): 有安 大典，久保 英実香，芝田 晋介，荒木 喜美
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: 神奈川県横浜市（パシフィコ横浜）
  • Year and Date: 2016-11-30
• [Presentation] CRISPR-Cas9を用いた遺伝子改変マウス作製 (2016)
  • Author(s): 荒木 喜美
  • Organizer: 第48回日本臨床分子形態学会総会・学術集会
  • Place of Presentation: 熊本県熊本市（くまもと県民交流館パレア）
  • Year and Date: 2016-09-23
  • Invited
• [Presentation] CRISPR-Cas9とゲノム編集で解き明かす尾部退行症候群の発症メカニズム (2016)
  • Author(s): 仙波 圭，大場 隆，片渕 秀隆，飯田 有俊，池川 志郎，荒木 正健，荒木 喜美
  • Organizer: 第48回日本臨床分子形態学会総会・学術集会
  • Place of Presentation: 熊本県熊本市（くまもと県民交流館パレア）
  • Year and Date: 2016-09-23
• [Presentation] Cre-driverマウスラインの樹立と解析（Cre-mER T2におけるタモキシフェンの投与方法について） (2016)
  • Author(s): 国場 訓，江藤 聡，荒木 美幸，森田 彩香，來海 葉子，山村 研一，吉信 公美子，荒木 喜美，荒木 正健
  • Organizer: 日本遺伝学会第88回大会
  • Place of Presentation: 静岡県三島市（日本大学）
  • Year and Date: 2016-09-07
• [Presentation] p53により誘導されるLincRNA-p21の生体内発現解析 (2016)
  • Author(s): 古畑 理樹，中原 舞，伊東 春香，荒木 正健，吉信 公美子，山村 研一，荒木 喜美
  • Organizer: 日本遺伝学会第88回大会
  • Place of Presentation: 静岡県三島市（日本大学）
  • Year and Date: 2016-09-07
• [Presentation] 染色体特異的にクラスターを形成しているトラップ領域（CSCT13）の解析 (2016)
  • Author(s): 荒木 正健，武田 伊世，大賀 俊範，江藤 聡，中原 舞，吉信 公美子，荒木 喜美
  • Organizer: 日本遺伝学会第88回大会
  • Place of Presentation: 静岡県三島市（日本大学）
  • Year and Date: 2016-09-07
• [Presentation] CSCT2領域（3.1Mbp）欠損マウスラインの樹立 (2016)
  • Author(s): 大賀 俊範，武田 伊世，江藤 聡，中原 舞，吉信 公美子，荒木 喜美，荒木 正健
  • Organizer: 日本遺伝学会第88回大会
  • Place of Presentation: 静岡県三島市（日本大学）
  • Year and Date: 2016-09-07
• [Presentation] Gene-trap mutagenesis is useful for analysis of long intergenic non-coding RNA genes in vivo (2016)
  • Author(s): Nakahara, M., Yoshinobu, K., Ito, H., Furuhata, R., Yamamura, K., Araki, M., Araki, K.
  • Organizer: The 2016 AMMRA & AMPC meeting
  • Place of Presentation: 神奈川県足柄下郡箱根町ホテルおかだ
  • Year and Date: 2016-05-20
  • Int'l Joint Research
• [Remarks] 熊本大学 生命資源研究・支援センター 疾患モデル分野
  • URL: http://irda-genetics.kuma-u.jp/