| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
When we produce iPS cells, a transcription of RNA from an inactivated X chromosome is induced. And the induction is insufficient in a partially-reprogrammed iPS cells. Thus, we tried to find regions from which the X chromosome reactivation starts and investigate a mechanism to produce well-reprogrammed iPS cells. We succeeded to develop SNP cDNA typing system which can determine the X chromosome reactivation and, by using the system, isolate iPS cells with different reactivation state. A transcriptional profile in the iPS cells made us identify the initiation regions of the X chromosome reactivation.
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