| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
The interaction between rhodopsin, a typical G-protein coupled receptor, and arrestin was studied by small-angle X-ray scattering using nanodiscs. The comparison of self-association and interaction with phosphorylated rhodopsin between rod arrestin and its splice variant p44 suggested that p44 forms tetramer similar to that of arrestin. In addition, it was experimentally demonstrated that p44 and phosphorylated rhodopsin form unstable complex (pre-coupled state) in the dark, which is converted to fully coupled state by light.
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