Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Outline of Final Research Achievements |
Chloroplast genomes in higher plants encode ~ 20 group II and 1 group I introns. The CRM domain (The chloroplast RNA splicing and ribosome maturation) is an RNA binding domain that is required for splicing of group II and group I introns in higher plant chloroplasts. This domain is derived from a prokaryotic 50S pre-ribosome binding protein, YhbY. It comprises 14 or 16 orthologous groups in rice and Arabidopsis, respectively. To explore the functional repertoire of CRM domain proteins, we studied CFM1 (for CRM Family Member 1) which harbors 3 CRM domains in rice and maize. RNA co-immunoprecipiation assay showed that CFM1 in maize associates with several group II introns in chloroplasts. A rice cfm1-1 mutant possesses a point mutation conditions white or white-variegated seedling. The cfm1-1 mutant impaired in the splicing of several group II introns in chloroplasts.
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