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The origin of androdioecy and genetic diversity of androgenetic clam Corbicula lumina

Research Project

Project/Area Number 16K07462
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Evolutionary biology
Research InstitutionMie University

Principal Investigator

Komaru Akira  三重大学, 生物資源学研究科, 教授 (10293804)

Co-Investigator(Kenkyū-buntansha) 遊佐 陽一  奈良女子大学, 自然科学系, 教授 (60355641)
河村 功一  三重大学, 生物資源学研究科, 教授 (80372035)
Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords雄性発生 / 自家受精 / 卵胎生 / タイワンシジミ / 雌雄同体 / 雄性異体 / androdioecy / 二枚貝 / 卵寄生 / 進化 / 遺伝学 / 繁殖
Outline of Final Research Achievements

The present study demonstrated thatWe conduct the 1) population analysis such as growth and maturity on the population including male and hermaphrodites, 2) comparison of spermatozoa produced by male and hermaphrodite, 3) microsatellite analysis of parents and its brooding juveniles. The self-fertilization rates were fluctuated among the hermaphrodites. These analyses demonstrated that the hermaphrodites accepted the spermatozoa from neighboring hermaphrodites and males. We propose the requirements for maintaining males, and for their successful reproduction in self-compatible hermaphrodite populations, to be: 1) high population density, 2) low self-fertilization rates of hermaphrodites; 3) more spermatozoa and greater survival of males than those of hermaphrodites, 4) simultaneous discharge of gametes from hermaphrodites and males.

Academic Significance and Societal Importance of the Research Achievements

本研究では親子判別、クローン性の等の遺伝解析、さらにフィールドにおける個体群動態調査を平行して行って、本集団が真のandrodioecyであることを明らかにすることができた。またandrodioecyが成り立つための条件についても 1)個体群密度が高い、2)雌雄同体と同時にオスの放精が起きる、3)自家受精率の低い同体集団、と言う条件下でないと雄が維持されることは困難、ということを明らかにすることができた。動物界で極めて稀な雄と雌雄同体という組み合わせ(雄性異体 androdioecy) が、維持されていることを明らかにすることができ、それが維持される条件について推察を行った。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (6 results)

All 2020 2018 2017 Other

All Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (1 results) (of which Int'l Joint Research: 1 results) Remarks (1 results) Funded Workshop (1 results)

  • [Journal Article] Genetic confirmation of “egg parasitism” in androgenetic freshwater <i>Corbicula</i> clams by paternity testing using microsatellite DNA markers2020

    • Author(s)
      Sano N., Houki S., Kodan A., Kawamura K., Yamada M. & Komaru A.
    • Journal Title

      Plankton and Benthos Research

      Volume: 15 Issue: 1 Pages: 58-62

    • DOI

      10.3800/pbr.15.58

    • NAID

      130007815684

    • ISSN
      1880-8247, 1882-627X
    • Year and Date
      2020-02-28
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The process of first polar body formation in eggs of the androgenetic clam Corbicula fluminea2018

    • Author(s)
      Hotta M & Komaru A
    • Journal Title

      J Shellfish Res

      Volume: 37 Issue: 1 Pages: 131-137

    • DOI

      10.2983/035.037.0111

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Meiotic and Early Zygotic Development in Crassostrea virginica Observed through Confocal Microscopy2017

    • Author(s)
      Hotta Masaru, Akira Komaru, Joana Sousa and Standish K. Allen, Jr
    • Journal Title

      Journal of shellfish research

      Volume: 36 Issue: 3 Pages: 699-706

    • DOI

      10.2983/035.036.0319

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] The process of two first polar body formation of androgenetic clam Corbicula fluminea eggs labeled with α-, γ-tubulin antibody, rhodamine-phalloidine, and DAPI, observed by confocal microscopy2017

    • Author(s)
      A Komaru, M Hotta
    • Organizer
      日本水産学会
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Remarks] 水圏資源生物学教室の業績一覧(三重大学生物資源学研究科水圏資源生物学研究室)

    • URL

      http://www.bio.mie-u.ac.jp/~kawa-k/

    • Related Report
      2019 Annual Research Report
  • [Funded Workshop] Intl symposium Fisheries Science for future generations2017

    • Related Report
      2017 Research-status Report

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Published: 2016-04-21   Modified: 2021-02-19  

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