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Elucidation of the mechanism for enzymatic browning associated with bacteria on fresh-cut produce

Research Project

Project/Area Number 16K07606
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Horticultural science
Research InstitutionKindai University

Principal Investigator

IZUMI Hidemi  近畿大学, 生物理工学部, 教授 (50168275)

Research Collaborator Inoue Ayano  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords酵素的褐変 / カット青果物 / ポリフェノールオキシダーゼ(PPO) / フェニルアラニンアンモニアリアーゼ(PAL) / ペルオキシダーゼ(POD) / 細菌数 / 細菌種 / Psedomonas fluorescens / ぺルオキシダーゼ(POD) / ポリフェノールオキシダーゼ(PPO) / ポリフェノール / リグニン / 衛生 / 園芸学 / 細菌 / 食品 / 微生物
Outline of Final Research Achievements

It is recognized that oxidative enzymatic browning of fresh-cut produce primarily involves the enzymatic activity of polyphenol oxidase (PPO). Bacterial counts on fresh-cut potatoes, apples, and lettuce were higher on browning tissues than on non-browning tissues. Bacterial species isolated from the browning tissues, not non-browning tissues, were identified as Pseudomonas fluorescens. When potato slices were initially inoculated with P. fluorescens and then stored at 5°C, browning reactions on potatoes were enhanced by an increase of plant PPO gene expression and PPO activity that was derived from potatoes rather than P. fluorescens. Thus, enzymatic browning is apparently involved in a plant-bacteria interaction through induction of plant PPO in plant defense reactions to attack by bacteria.

Academic Significance and Societal Importance of the Research Achievements

カット青果物の品質低下の大きな要因として、褐変の発生と微生物の増殖が挙げられる。従来は、酵素的褐変は植物体内の生化学的反応として、また腐敗の原因である微生物増殖は付着微生物個体の生物的成長として捉え、それぞれ別の制御手法で、品質保持を図ってきた。しかし、本研究では、褐変に関わる酵素および褐変関連物質とそれらを生成あるいは刺激する微生物との関連性に注目し、微生物が関与する褐変の機構を解明した。カット青果物の褐変の発生および促進を誘導する特定の微生物を同定できたことから、カット青果物の品質保持のための褐変抑制と微生物制御を一元的に捉え、これらを同時に行う新しい鮮度保持技術の構築も可能となる。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (8 results)

All 2019 2018 2017 2016

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (5 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results) Book (2 results)

  • [Journal Article] Quality and safety of fresh horticultural commodities: Recent advances and future perspectives2017

    • Author(s)
      Pramod V. Mahajan, Oluwafemi J. Caleb, Maria I. Gil, Hidemi Izumi, Giancarlo Colelli, Christopher B. Watkins, Manuela Zude
    • Journal Title

      Food Packaging and Shelf Life

      Volume: 14 Pages: 2-11

    • DOI

      10.1016/j.fpsl.2017.08.001

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Influence of artificial bacterial inoculation on enzymatic browning of fresh-cut potatoes and apples2018

    • Author(s)
      Hidemi Izumi、Natsuki Takebe、Kotoha Inui、Ayano Inoue
    • Organizer
      American Society for Horticultural Science (ASHS) 2018 Annual Conference
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 青果物/カット青果物の微生物制御と品質保持2018

    • Author(s)
      泉 秀実
    • Organizer
      テックデザイン講習会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Disinfectant treatments to reduce microbial food safety risks for fresh and fresh-cut produce2017

    • Author(s)
      Hidemi Izumi
    • Organizer
      IV ISHS Asia Symposium on Quality Management in Postharvest Systems
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] カットジャガイモ褐変部位から単離されたPseudomonas fluorescensが酵素的褐変に及ぼす影響2017

    • Author(s)
      井上あやの、泉 秀実
    • Organizer
      日本防菌防黴学会第44回年次大会
    • Related Report
      2017 Research-status Report
  • [Presentation] カットジャガイモの酵素的褐変と微生物との関連性2016

    • Author(s)
      井上あやの、木戸裕之、泉 秀実
    • Organizer
      日本防菌防黴学会第43回年次大会
    • Place of Presentation
      きゅりあん(品川区立総合区民会館)(東京都・品川区)
    • Year and Date
      2016-09-26
    • Related Report
      2016 Research-status Report
  • [Book] Controlled and Modified Atmospheres for Fresh and Fresh-Cut Produce (Chapter 6. CA/MA requirements for spoilage microorganisms and human pathogens)2019

    • Author(s)
      Hidemi Izumi
    • Total Pages
      42
    • Publisher
      Elsevier
    • ISBN
      9780128045992
    • Related Report
      2018 Annual Research Report
  • [Book] Fresh-Cut Fruits and Vegetables: Technology, Physiology and Safety2016

    • Author(s)
      Izumi, H., Rodov, V., Bai, J., and Wendakoon, S.K.
    • Publisher
      CRC Press
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2025-11-18  

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