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The analysis of microRNA localization using fluorescence resonance energy transfer based in situ hybridization

Research Project

Project/Area Number 16K08471
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field General anatomy (including histology/embryology)
Research InstitutionUniversity of Miyazaki

Principal Investigator

Hishikawa Yoshitaka  宮崎大学, 医学部, 教授 (60304276)

Co-Investigator(Kenkyū-buntansha) 石塚 匠  宮崎大学, 医学部, 助教 (50700085)
川口 真紀子  宮崎大学, 医学部, 助教 (90405598)
チョウジョウフ ナランツオツク  宮崎大学, 医学部, 講師 (90640962)
ピュー シンウー  宮崎大学, 医学部, 助教 (90718123)
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordsin situハイブリダイゼーション / マイクロRNA / 蛍光 / 蛍光共鳴エネルギー移動 / in situ hybridization / 28S rRNA / microRNA / miR122 / ラット肝切除モデル / パラフィン切片 / 凍結切片 / in situ hybidization / miR-122 / ラット肝臓 / 解剖学 / 細胞組織化学
Outline of Final Research Achievements

We have developed a new application of fluorescence resonance energy transfer based molecular beacon probe for in situ hybridization (FRET-ISH) for detection of specific microRNA at the tissue and cellular level.

Academic Significance and Societal Importance of the Research Achievements

がんを含む様々な疾病に関与する小分子非翻訳RNAであるmicroRNA (miRNA)について、組織や細胞での発現動態を解析する新たな検出法として、FRET based in situハイブリダイゼーション(FRET-ISH)法を用いた高感度miRNA 局在検出法を開発した。このFRET-ISH法は、様々なmiRNAの特定の細胞や組織での細胞生物学的役割や疾病への関与について新たな知見が得られる有用な方法論であると考える。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (31 results)

All 2019 2018 2017 2016 Other

All Int'l Joint Research (2 results) Journal Article (17 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 16 results,  Open Access: 13 results,  Acknowledgement Compliant: 4 results) Presentation (11 results) (of which Int'l Joint Research: 4 results,  Invited: 4 results) Remarks (1 results)

  • [Int'l Joint Research] Department of Medical Research/Pathein University/Department of Traditional Medicine(ミャンマー)

    • Related Report
      2016 Research-status Report
  • [Int'l Joint Research] Mongolian Nat. Univ. of Med. Sciences(モンゴル)

    • Related Report
      2016 Research-status Report
  • [Journal Article] The HDAC Inhibitor, SAHA, Combined with Cisplatin Synergistically Induces Apoptosis in Alpha-fetoprotein-producing Hepatoid Adenocarcinoma Cells2019

    • Author(s)
      Kyaw MTH, Yamaguchi Y, Choijookhuu N, Yano K, Takagi H, Takahashi N, Synn Oo P, Sato K, Hishikawa Y.
    • Journal Title

      ACTA HISTOCHEMICA ET CYTOCHEMICA

      Volume: 52 Issue: 1 Pages: 1-8

    • DOI

      10.1267/ahc.18044

    • NAID

      130007605543

    • ISSN
      0044-5991, 1347-5800
    • Year and Date
      2019-02-28
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] HMGB2 is a novel adipogenic factor that regulates ectopic fat infiltration in skeletal muscles.2018

    • Author(s)
      Lee D, Taniguchi N, Sato K, Choijookhuu N, Hishikawa Y, Kataoka H, Morinaga H, Lotz M, Chosa E.
    • Journal Title

      Sci rep

      Volume: 8 Issue: 1 Pages: 9601-9601

    • DOI

      10.1038/s41598-018-28023-7

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Pivotal Role of IL-22 Binding Protein in the Epithelial Autoregulation of Interleukin-22 Signaling in the Control of Skin Inflammation2018

    • Author(s)
      Tomohiro Fukaya, Takehito Fukui, Tomofumi Uto, Hideaki Takagi, Junta Nasu, Noriaki, Miyanaga, Keiichi Arimura, Takeshi Nakamura, Haruhiko Koseki, Narantsog Choijookhuu, Yoshitaka Hishikawa, Katsuaki Sato
    • Journal Title

      Frontiers in Immunology

      Volume: 9 Pages: 1-13

    • DOI

      10.3389/fimmu.2018.01418

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The HDAC Inhibitor, SAHA, Prevents Colonic Inflammation by Suppressing Pro-inflammatory Cytokines and Chemokines in DSS-induced Colitis2018

    • Author(s)
      Ali MN, Choijookhuu N, Takagi H, Srisowanna N, Nguyen Nhat Huynh M, Yamaguchi Y, Synn Oo P, Tin Htwe Kyaw M, Sato K, Yamaguchi R, Hishikawa Y.
    • Journal Title

      ACTA HISTOCHEMICA ET CYTOCHEMICA

      Volume: 51 Issue: 1 Pages: 33-40

    • DOI

      10.1267/ahc.17033

    • NAID

      130006405039

    • ISSN
      0044-5991, 1347-5800
    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Estrogen Regulates Mitochondrial Morphology through Phosphorylation of Dynamin-related Protein 1 in MCF7 Human Breast Cancer Cells2018

    • Author(s)
      Oo PS, Yamaguchi Y, Sawaguchi A, Kyaw MTH, Choijookhuu N, Ali MN, Srisowanna N, Hino S, Hishikawa Y.
    • Journal Title

      ACTA HISTOCHEMICA ET CYTOCHEMICA

      Volume: 51 Issue: 1 Pages: 21-31

    • DOI

      10.1267/ahc.17034

    • NAID

      130006405038

    • ISSN
      0044-5991, 1347-5800
    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Critical role of plasmacytoid dendritic cells in induction of oral tolerance.2018

    • Author(s)
      Uto T, Takagi H, Fukaya T, Nasu J, Fukui T, Miyanaga N, Arimura K, Nakamura T, Choijookhuu N, Hishikawa Y, Sato K.
    • Journal Title

      J Allergy Clin. Immunol.

      Volume: 印刷中 Issue: 6 Pages: 2156-2167.e9

    • DOI

      10.1016/j.jaci.2017.11.048

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Photodynamic therapy of human biliary cancer cell line using combination of phosphorus porphyrins and light emitting diode2017

    • Author(s)
      Jin Matsumoto, Kou Suzuki, Masahide Yasuda, Yuya Yamaguchi, Yoshitaka Hishikawa, Naoya Imamura, Atsushi Nanashima
    • Journal Title

      Bioorganic & Medicinal Chemistry

      Volume: 25 Issue: 24 Pages: 6536-6541

    • DOI

      10.1016/j.bmc.2017.10.031

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Novel role of Rac-Mid1 signaling in medial cerebellar development.2017

    • Author(s)
      Nakamura T, Ueyama T, Ninoyu Y, Sakaguchi H, Choijookhuu N, Hishikawa Y, Kiyonari H, Kohta M, Sakahara M, de Curtis I, Kohmura E, Hisa Y, Aiba A, Saito N.
    • Journal Title

      Development

      Volume: 144 Issue: 10 Pages: 1863-1875

    • DOI

      10.1242/dev.147900

    • NAID

      120006363303

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Flexible adaptation of male germ cells from female iPSCs of endangered Tokudaia osimensis2017

    • Author(s)
      Arata Honda, Narantsog Choijookhuu, Haruna Izu, Yoshihiro Kawano, Mizuho Inokuchi, Kimiko Honsho, Ah-Reum Lee, Hiroki Nabekura, Hiroshi Ohta, Tomoyuki Tsukiyama, Yasuhide Ohinata, Asato Kuroiwa, Yoshitaka Hishikawa, Mitinori Saitou, Takamichi Jogahara, Chihiro Koshimoto
    • Journal Title

      Science Advances

      Volume: - Issue: 5

    • DOI

      10.1126/sciadv.1602179

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Arsenic trioxide induces ROS activity and DNA damage, leading to G0/G1 extension in skin fibroblasts through the ATM-ATR-associated Chk pathway.2017

    • Author(s)
      Chayapong J, Madhyastha H, Madhyastha R, Nurrahmah QI, Nakajima Y, Choijookhuu N, Hishikawa Y, Maruyama M.
    • Journal Title

      Environ Sci Pollut Res Int.

      Volume: 24 Issue: 6 Pages: 5316-5325

    • DOI

      10.1007/s11356-016-8215-7

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Discrimination of stem cell status after subjecting cynomolgus monkey pluripotent stem cells to naive conversion.2017

    • Author(s)
      Honda A, Kawano Y, Izu H, Choijookhuu N, Honsho K, Nakamura T, Yabuta Y, Yamamoto T, Takashima Y, Hirose M, Sankai T, Hishikawa Y, Ogura A, Saitou M
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 45285-45285

    • DOI

      10.1038/srep45285

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Frequency of CD4+CD161+ T Cell and Interleukin-10 Expression in Inflammatory Bowel Diseases2017

    • Author(s)
      Tsuchiya K, Ikeda T, Batmunkh B, Choijookhuu N, Ishizaki H, Hotokezaka M, Hishikawa Y, Nanashima A.
    • Journal Title

      ACTA HISTOCHEMICA ET CYTOCHEMICA

      Volume: 50 Issue: 1 Pages: 21-28

    • DOI

      10.1267/ahc.16035

    • NAID

      130005399467

    • ISSN
      0044-5991, 1347-5800
    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Estrogen Accelerates Cell Proliferation through Estrogen Receptor α during Rat Liver Regeneration after Partial Hepatectomy2017

    • Author(s)
      Batmunkh B, Choijookhuu N, Srisowanna N, Byambatsogt U, Synn Oo P, Noor Ali M, Yamaguchi Y, Hishikawa Y.
    • Journal Title

      ACTA HISTOCHEMICA ET CYTOCHEMICA

      Volume: 50 Issue: 1 Pages: 39-48

    • DOI

      10.1267/ahc.17003

    • NAID

      130005399468

    • ISSN
      0044-5991, 1347-5800
    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The role of estrogen receptors in intestinal homeostasis and disease.2016

    • Author(s)
      Choijookhuu N, Hino SI, Hishikawa Y.
    • Journal Title

      Receptors Clin Investig.

      Volume: in press

    • DOI

      10.14800/rci.1109

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Arsenic acid inhibits proliferation of skin fibroblasts, and increases cellular senescence through ROS mediated MST1-FOXO signaling pathway2016

    • Author(s)
      Yamaguchi Y, Madhyastha H, Madhyastha R, Choijookhuu N, Hishikawa Y, Pengjam Y, Nakajima Y, Maruyama M
    • Journal Title

      The Journal of Toxicological Sciences

      Volume: 41 Issue: 1 Pages: 105-113

    • DOI

      10.2131/jts.41.105

    • NAID

      130005119779

    • ISSN
      0388-1350, 1880-3989
    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Plasmacytoid dendritic cells orchestrate TLR7-mediated innate and adaptive immunity for the initiation of autoimmune inflammation2016

    • Author(s)
      Hideaki Takagi, Keiichi Arimura, Tomofumi Uto, Tomohiro Fukaya, Takeshi Nakamura, Narantsog Choijookhuu, Yoshitaka Hishikawa, Katsuaki Sato
    • Journal Title

      Scientific Reports

      Volume: 6 Issue: 1 Pages: 24477-24477

    • DOI

      10.1038/srep24477

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] 遺伝子の局在を見るーin situ hybridization2016

    • Author(s)
      菱川善隆
    • Journal Title

      Clinical Neuroscience

      Volume: 34 Pages: 625-628

    • Related Report
      2016 Research-status Report
  • [Presentation] A new approach for FRET based in situ hybridization2019

    • Author(s)
      Narantsog Choijookhuu、菱川善隆
    • Organizer
      第13回日中合同組織細胞化学セミナー
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] FRETを用いたin situハイブリダイゼーションによる遺伝子発現解析2018

    • Author(s)
      菱川善隆
    • Organizer
      日本顕微鏡学会第74回学術講演会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] A new strategy of FRET based molecular beacon fluorescent probe for in situ hybridization.2017

    • Author(s)
      Choijookhuu N ,石塚匠,徐岩,小路武彦,菱川善隆
    • Organizer
      第122回日本解剖学会総会・全国学術集会
    • Place of Presentation
      長崎市
    • Year and Date
      2017-03-28
    • Related Report
      2016 Research-status Report
  • [Presentation] FRET(蛍光共鳴エネルギー移動)標識プローブによる高感度ISH法の有用性2017

    • Author(s)
      菱川善隆
    • Organizer
      第58回日本組織細胞化学会総会・学術集会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] The effect of estrogen and its receptor(ERα)in the liver regeneration after partial hepatectomy.2017

    • Author(s)
      菱川善隆
    • Organizer
      The12th China-Japan joint Seminar on Histochemistry and Cytochemistry
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Role of estrogen on mitochondrial dynamic through Drp1 and P-Drp1 in human breast cancer cell line.2016

    • Author(s)
      Phyu Synn Oo, Akira Sawaguchi,山口優也, Narantsog Choijookhu, Baatarsuren Batmunk,菱川善隆
    • Organizer
      第3回ミャンマーー日本シンポジウム in パテイン大学
    • Place of Presentation
      パテイン、ミャンマー
    • Year and Date
      2016-12-03
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] FRET based in situ hybridization: Advanced molecular histochemical approach2016

    • Author(s)
      Hishikawa Y
    • Organizer
      第3回ミャンマーー日本シンポジウム in パテイン大学
    • Place of Presentation
      パテイン、ミャンマー
    • Year and Date
      2016-12-03
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] A new strategy: FRET based molecular beacon fluorescent probe for in situ hybridization.2016

    • Author(s)
      Choijookhuu N ,石塚匠,徐岩,小路武彦,菱川善隆
    • Organizer
      第58回 日本顕微鏡学会九州支部学術講演会
    • Place of Presentation
      北九州市
    • Year and Date
      2016-12-03
    • Related Report
      2016 Research-status Report
  • [Presentation] Possible regulation of estrogen and estrogen receptor alpha during liver regeneration2016

    • Author(s)
      Baatarsuren Batmunkh, Narantsog Choijookhuu, Phyu Synn Oo, Naparee Srisowanna,山口優也, 菱川善隆
    • Organizer
      第48回日本臨床分子形態学会・学術集会
    • Place of Presentation
      熊本市
    • Year and Date
      2016-09-23
    • Related Report
      2016 Research-status Report
  • [Presentation] A new approach: FRET based molecular beacon fluorescent probe for in situ hybridization.2016

    • Author(s)
      Choijookhuu N ,石塚匠,徐岩,小路武彦,菱川善隆
    • Organizer
      第57回日本組織細胞化学会総会・学術集会
    • Place of Presentation
      三鷹市
    • Year and Date
      2016-09-03
    • Related Report
      2016 Research-status Report
  • [Presentation] RET Based Molecular Beacon Fluorescent Probe For In Situ Hybridization.2016

    • Author(s)
      Choijookhuu N ,石塚匠,徐岩,小路武彦,菱川善隆
    • Organizer
      日本顕微鏡学会第72回学術講演会
    • Place of Presentation
      仙台市
    • Year and Date
      2016-06-14
    • Related Report
      2016 Research-status Report
  • [Remarks] 宮崎大学医学部解剖学講座組織細胞化学分野

    • URL

      http://www.med.miyazaki-u.ac.jp/home/anatomy1/

    • Related Report
      2018 Annual Research Report 2017 Research-status Report 2016 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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