Analysis of Hippo-YAP mediated 3D organogenesis
| Project/Area Number |
16K08588
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General medical chemistry
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| Research Institution | Yamaguchi University |
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Principal Investigator |
ASAOKA Yoichi 山口大学, 大学院医学系研究科, 講師 (10436644)
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| Project Period (FY) |
2016-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 細胞内シグナル伝達 / 三次元器官構築 / YAP / メカノホメオスターシス / 小型魚類 / YAPメカノホメオスターシス / 器官構築 |
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| Outline of Final Research Achievements |
In order to clarify the YAP molecular network that controls 3D organogenesis, we performed a comparative transcriptome analysis between the wild type and hir mutant medaka using the RNA-seq, and identified several genes that exhibited the characteristic expression pattern in hir mutant. KO fish were generated by using the CRISPR-Cas9 system for YAP molecular network candidates. To date, several KO fish have been established and their phenotypes are being analyzed.
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| Academic Significance and Societal Importance of the Research Achievements |
今回のメダカhir変異体を用いたRNA-seq解析により、YAPの下流標的遺伝子群を網羅的に明らかにすることができた。特にhir変異体において顕著な発現変動を示す上位50遺伝子の中には、インテグリンシグナルやアクチンの重合・脱重合に関与する遺伝子群が含まれており、YAPメカノホメオスターシスを支える重要因子の可能性がある。今後、これまでに作出に成功したノックアウト変異体の詳細な表現型解析をさらに進めることにより、YAPメカノホメオスターシスの分子基盤の一層の理解につながるとともに、YAPメカノ分子ネットワークの破綻がどのように疾患の発症・増悪へ関与するのかを明らかにできると考えている。
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Report
(5 results)
Research Products
(21 results)
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[Journal Article] The clock components Period2, Cryptochrome1a, and Cryptochrome2a function in establishing light-dependent behavioral rhythms and/or total activity levels in zebrafish.2019
Author(s)
Hirayama J, Alifu Y, Hamabe R, Yamaguchi S, Tomita J, Maruyama Y, Asaoka Y, Nakahama KI, Tamaru T, Takamatsu K, Takamatsu N, Hattori A, Nishina S, Azuma N, Kawahara A, Kume K, Nishina H.
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Journal Title
Sci Rep
Volume: 9
Pages: 1-15
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] The mevalonate pathway regulates primitive streak formation via protein farnesylation2016
Author(s)
Okamoto-Uchida Y, Yu R, Miyamura N, Arima N, Ishigami-Yuasa M, Kagechika H, Yoshida S, Hosoya T, Nawa M, Kasama T, Asaoka Y, Alois RW, Elling U, Penninger JM, Nishina S, Azuma N, Nishina H
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Journal Title
Scientific Reports.
Volume: 6
Pages: 37697-37697
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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