Identification for radiation signature of cancer risk in irradiated thyroid
Project/Area Number |
16K08714
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Experimental pathology
|
Research Institution | Nagasaki University |
Principal Investigator |
|
Project Period (FY) |
2016-04-01 – 2019-03-31
|
Project Status |
Completed (Fiscal Year 2018)
|
Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
Keywords | 放射線被ばく / 発がん / 甲状腺 / 分子病理学 / バイオマーカー / 放射線発がん / 発がんリスク / ゲノム不安定性 |
Outline of Final Research Achievements |
This study aimed to comprehensively examine the expression profile of genes altering in thyroid after irradiation (IR) by rat model and to identify biomarker in irradiated (IR) thyroid to estimate the cancer risk of individuals exposed to IR. Expression profile of mRNA in thyroid after IR were assessed by microarray technique, expression level of candidate for biomarker was quantitatively confirmed by digital PCR, and their usefulness as a biomarker was finally validated by a blinded experiment. In results, it was indicated that expression level of several molecules was abnormally altered in IR thyroid at precancerous stage. In particular, the level of CDKN1a expression was significantly increased in IR thyroid, and adopting 11.7-fold increase as a cutoff value to distinguish IR from non-IR, the sensitivity and specificity values were 60% and 100%, respectively. Thus, analysis for expression of these candidates may provide a biological evidence for IR effect in respective thyroids.
|
Academic Significance and Societal Importance of the Research Achievements |
被爆者疫学研究では甲状腺がんを始めとする人体における発がん影響が明らかにされていて、福島原発事故後の被曝健康影響の議論がなされてきた。疫学的データでは集団を対象とした被曝影響のリスク比として表現され、個人への放射線影響の評価は曖昧となるため、生物学的エビデンスを基盤とする放射線影響リスク評価の方法論の確立が喫緊の課題となる。本研究では、遺伝子発現の網羅的解析により、被曝甲状腺では前がん状態から分子異常が亢進していて、発がんリスクを反映することを初めて実証した。ラットモデルではCDKN1aが発がんリスク予測のバイオマーカーとして有用で、本法はヒトでのリスク評価への応用に期待できる。
|
Report
(4 results)
Research Products
(57 results)
-
-
[Journal Article] Sporadic pediatric papillary thyroid carcinoma harboring the ETV6/NTRK3 fusion oncogene in a 7-year-old Japanese girl: a case report and review of literature.2018
Author(s)
Otsubo R, Mussazhanova Z, Akazawa Y, Sato A, Matsuda K, Matsumoto M, Yano H, Matsuse M, Mitsutake N, Ando T, Niino D, Nagayasu T, Nakashima M.
-
Journal Title
J Pediatr Endocrinol Metab.
Volume: 31
Pages: 461-467
DOI
Related Report
Peer Reviewed / Int'l Joint Research
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Journal Article] Toyocamycin attenuates free fatty acid-induced hepatic steatosis and apoptosis in cultured hepatocytes and ameliorates nonalcoholic fatty liver disease in mice2017
Author(s)
Ikuko Takahara, Yuko Akazawa, Maiko Tabuchi, Katsuya Matsuda, Hisamitsu Miyaaki, Youko Kido, Yasuko Kanda, Naota Taura, Ken Ohnita, Fuminao Takeshima, Yusuke Sakai, Susumu Egushi, Masahiro Nakashima, Kazuhiko Nakao
-
Journal Title
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
-
[Journal Article] Internal exposure to neutron-activated 56Mn dioxide powder in Wistar rats - Part 2: Pathological effects2017
Author(s)
Shichijo K, Fujimoto N, Uzbekov D, Kairkhanova Y, Saimova A, Chaizhunusova N, Sayakenov N, Shabdarbaeva D, Aukenov N, Azimkhanov A, Kolbayenkov A, Mussazhanova J, Niino D, Nakashima M, Zhumadilov K, Stepanenko V, Tomonaga M, Rakhypbekov T, Hoshi M
-
Journal Title
Radiation and Environmental Biophysics
Volume: 56
Pages: 55-61
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
-
[Journal Article] Internal exposure to neutron-activated 56Mn dioxide powder in Wistar rats - Part 1: Dosimetry-2017
Author(s)
Stepanenko V, Rakhypbekov T, Otani K, Endo S, Satoh K, Kawano N, Shichijo K, Nakashima M, Takatsuji T, Sakaguchi A, Kato H, Onda Y, Fujimoto N, Toyoda S, Sato A, Chaizhunusova N, Uzbekov D, Saimova A, Shabdarbaeva D, Skakov M, Zhumadilov K, Kairikhanova A, Skvortsov V, Bogacheva V, Hoshi M, et al.
-
Journal Title
Radiation and Environmental Biophysics
Volume: 56
Pages: 47-54
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
-
-
[Journal Article] Association between p53-binding protein 1 expression and genomic instability in oncocytic follicular adenoma of the thyroid2016
Author(s)
Mussazhanova Z, Akazawa Y, Matsuda K, Shichijo K, Miura S, Otsubo R, Oikawa M, Yoshiura K, Mitsutake N, Rogounovitch T, Saenko V, Kozykenova Z, Zhetpisbaev B, Shabdarbaeva D, Sayakenov N, Amntayev B, Kondo H, Ito M, Nakashima M.
-
Journal Title
Endocr J
Volume: 63
Issue: 5
Pages: 457-467
DOI
NAID
ISSN
0918-8959, 1348-4540
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-