Analysis of the diversity of collagen disease treatment response using recombinant inbred mice
| Project/Area Number |
16K08735
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Experimental pathology
|
|---|
| Research Institution | Gifu University |
|---|
Principal Investigator |
|
|---|
| Research Collaborator |
SUZUI Natsuko
KOBAYASHI Kazuhiro
NODA Touko
Gröne Hermann-Josef
|
|---|
| Project Period (FY) |
2016-04-01 – 2019-03-31
|
| Project Status |
Completed (Fiscal Year 2018)
|
| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
|---|
| Keywords | 病理学 / ゲノム / 薬剤反応性 / 炎症・膠原病 / モデル動物 / オステオオンチン / オステオポンチン / 蛋白アナログ |
|---|
| Outline of Final Research Achievements |
In order to establish a diversity model of therapeutic response to collagen diseases, recombinant inbred MXH/lpr mice were bred and returned from frozen fertilized eggs to provide mice for experiments. Among Opn and autoantigen-inhibiting proteins, proteins containing a binding epitope with Opn were analyzed and synthesized by a wheat germ cell-free protein synthesis system. In parallel with the in vitro analysis, we developed a drug delivery system (DDS) by lymphatic administration. Cyclophosphamide was administered to patients with glomerulonephritis. Cyclophosphamide was administered to patients with glomerulonephritis. DDS was established, and the effect of treatment was confirmed and adverse events were analyzed.
|
| Academic Significance and Societal Importance of the Research Achievements |
本研究により、多因子疾患である膠原病の治療多様性モデルが確立し、新薬開発等における実験モデルとして有用であることが明らかとなった。
また、膠原病治療のためのリンパ節への薬剤投与という新たなdrug delivery systemの開発を果たし得た。
さらに、Opnの多型部位阻害蛋白の開発は、膠原病治療の新たな治療戦略につながる大きなシーズとなった。今後さらに蛋白製剤の低分子化をすすめ、治療薬として実用化できる方策を進めることが期待される。
|
Report
(4 results)
Research Products
(26 results)
-
-
-
-
-
[Journal Article] Strain differences in the proteome of dioxin-sensitive and -resistant mice treated with 2,3,7,8-tetrabromodibenzo-p-dioxin2017
Author(s)
Nguyen, H.T., Lauan Tsuchiya, M.C., Yoo, J., Iida, M., Agusa, T., Hirano, M., Kim, E.-Y., Miyazaki, T., Nose, M., Iwata, H.
-
Journal Title
Archives of Toxicology
Volume: 4
Issue: 4
Pages: 1763-1782
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
-
[Journal Article] Non-alcoholic steatohepatitis-related liver tumorigenesis is suppressed in mice lacking hepatic retinoid storage2017
Author(s)
Ideta, T. Shirakami, Y. Ohnishi, M. Maruta, A. Obara, K. Miyazaki, T. Kochi, T. Sakai, H. Tomita, H. Tanaka, T. Blaner, W. S. Shimizu, M.
-
Journal Title
Oncotarget
Volume: 8
Issue: 41
Pages: 70695-70706
DOI
Related Report
Peer Reviewed / Open Access
-
[Journal Article] TAFRO syndrome: 2 cases and review of the literature2017
Author(s)
Kawashima, M. Usui, T. Okada, H. Mori, I. Yamauchi, M. Ikeda, T. Kajita, K. Kito, Y. Miyazaki, T. Fujioka, K. Ishizuka, T. Morita, H.
-
Journal Title
Mod Rheumatol
Volume: 27
Issue: 6
Pages: 1093-1097
DOI
Related Report
Peer Reviewed
-
-
[Journal Article] Promotion of cell proliferation by the proto-oncogene DEK enhances oral squamous cell carcinogenesis through field cancerization2017
Author(s)
Nakashima, T. Tomita, H. Hirata, A. Ishida, K. Hisamatsu, K. Hatano, Y. Kanayama, T. Niwa, A. Noguchi, K. Kato, K. Miyazaki, T. Tanaka, T. Shibata, T. Hara, A.
-
Journal Title
Cancer Med
Volume: 6
Issue: 10
Pages: 2424-2439
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
