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Female-specific gene regulation in malaria parasites by an AP2-family transcription factor

Research Project

Project/Area Number 16K08758
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Parasitology (including sanitary zoology)
Research InstitutionMie University

Principal Investigator

Kaneko Izumi  三重大学, 医学系研究科, 助教 (20515720)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsマラリア / マラリア原虫
Outline of Final Research Achievements

The malarial gametocyte, the gamete precursor, is the parasite stage obligatory for malarial transmission to the mosquito vector. We report that an AP2-family transcription factor, AP2-F, is responsible for female-specific gene regulation. AP2-F expression in Plasmodium berghei was observed exclusively in female gametocytes. AP2-F disruption resulted in the arrest of female maturation, but did not affect the development of males. ChIP-seq analysis showed that AP2-FG directly regulates over 750 genes. Its targets include genes for female gametocyte-specific functions, such as gametogenesis, fertilization, and zygote development. AP2-F binding to target gene promoters was associated with a 10-bp sequence motif. These results indicate that AP2-F plays a role in the differentiation of early gametocytes into mature females by governing a female-specific gene expression repertoire.

Academic Significance and Societal Importance of the Research Achievements

本研究ではマラリア原虫のヒトから蚊への伝播を担うステージである雌性ガメトサイトを対象とし、一つの転写因子AP2-Fを手掛かりとすることで、雌性ガメトサイトで発現される遺伝子群を同定した。本研究で得られた成果は、マラリア伝播阻止戦略における新たな候補抗原開発に貢献する。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (3 results)

All 2017 2016

All Journal Article (3 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 3 results,  Open Access: 3 results)

  • [Journal Article] A potent malaria vaccine based on adenovirus with dual modifications at Hexon and pVII.2017

    • Author(s)
      Shiratsuchi T, Rai U, Kaneko I, Zhang M, Iwanaga S, Yuda M, Tsuji M.
    • Journal Title

      vaccine

      Volume: 15;35(50) Pages: 6990-7000

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A potent adjuvant effect of a CD1d-binding NKT cell ligand in human immune system mice.2017

    • Author(s)
      Li X, Huang J, Kaneko I, Zhang M, Iwanaga S, Yuda M, Tsuji M.
    • Journal Title

      Expert Rev Vaccines.

      Volume: 16(1): Pages: 73-80

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] A highly infectious Plasmodium yoelii parasite, bearing Plasmodium falciparum circumsporozoite protein2016

    • Author(s)
      Zhang M, Kaneko I, Tsao T, Mitchell R, Nardin EH, Iwanaga S, Yuda M, Tsuji M.
    • Journal Title

      Malar J

      Volume: 201 Issue: 1 Pages: 15-15

    • DOI

      10.1186/s12936-016-1248-z

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research

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Published: 2016-04-21   Modified: 2020-03-30  

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