Chararacteristics and pathogenicity of Escherichia albertii
| Project/Area Number |
16K08781
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bacteriology (including mycology)
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| Research Institution | Kagoshima University |
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Principal Investigator |
Ooka Tadasuke 鹿児島大学, 医歯学域医学系, 講師 (50363594)
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| Co-Investigator(Kenkyū-buntansha) |
藺牟田 直子 鹿児島大学, 医歯学域医学系, 助教 (00643470)
西 順一郎 鹿児島大学, 医歯学域医学系, 教授 (40295241)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | Escherichia albertii / 病原性 / タイピング / ゲノム / 運動性 / O抗原 / III型分泌系 / 鞭毛機能 / 病原機構 / 細菌 / 感染症 |
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| Outline of Final Research Achievements |
Escherichia albertii is a recently recognized human enteropathogen. The virulence mechanism and characteristics of the species have not yet been understood. In this study, we performed the functional analysis of the virulence-related and species-specific genes that we previously identified from the genome analysis of multiple E. albertii strains.
The results revealed that 1)E. albertii possessed the ability of invasion and biofilm formation but the E. coli type III secretion system 2 encoding ETT2 region was not involved in these mechanisms, 2)the inter-species horizontal gene transfer of the Stx2f phage between E. albertii and E.coli, and 3) the 40 O genotypes of E. albertii O antigen were defined. In addition, we developed PCR-based O genotyping system of E. albertii.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究では、近年、多数の集団感染事例の原因となっている新興下痢症起因菌E. albertiiについて、ゲノムから得た知見を基に解析を進め、その機構を明らかにするには至らなかったものの腸管病原性大腸菌や腸管出血性大腸菌とは異なる細胞侵入能を持つことを明らかにした。また、走化性関連因子を持たないにも関わらず環境中で運動能を示す可能性を示すことを明らかにし、本菌の感染経路を推定する上で非常に大きなヒントを得ることができたことは、今後の感染症対策に関する行政の観点からも重要である。加えて、40種類のO抗原遺伝子型を同定し、その遺伝子タイピング法を構築できたことも、本菌の特性を知る上で有用な情報である。
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Report
(3 results)
Research Products
(8 results)
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[Presentation] Genomic comparison of Stx2f phages from Escherichia coli and Escherichia albertii.2018
Author(s)
Tadasuke Ooka, Md. Rakibul Islam, Shoko Ochiai, Yoshitoshi Ogura, Kazuko Seto, Junko Isobe, Tetsuya Ikeda, Junji Seto, Yasuhiro Gotoh, Junichiro Nishi, Tetsuya Hayashi
Organizer
10th International Symposium on Shiga Toxin (Verocytotoxin) Producing Escherichia Coli Infections
Related Report
Int'l Joint Research
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