Morphology and host responses controlled by glycopeptidolipid in Mycobacterium avium-intracelluare complex
| Project/Area Number |
16K08792
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bacteriology (including mycology)
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| Research Institution | Tezukayama University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
前田 伸司 北海道科学大学, 薬学部, 教授 (50250212)
綾田 稔 大阪市立大学, 大学院医学研究科, 准教授 (90222702)
中 崇 帝塚山大学, 現代生活学部, 准教授 (40426599)
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| Project Period (FY) |
2016-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 非結核性抗酸菌症 / glycopeptidolipid / MAC症 / 糖ペプチド脂質 |
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| Outline of Final Research Achievements |
Mycobacterium avium-intracellulare complex (MAC) is classified into 28 serotypes based on the serotype-specific glycopeptidolipid (GPL) antigen. M. intracellulare Ku11 strain was isolated from a patient in Kumamoto, and produced a novel GPL. The Ku11-GPL was composed of 6 pieces of non-acylated sugars. The gene cluster involved in GPL biosynthesis was isolated and sequenced. We checked the similarity of the deduced amino acid sequences in the open reading frames (ORFs). In this study, it was identified the two ORFs that are functionally responsible for the elongation of oligosaccharide, glycosyltransferase. In addition, it is clarified that GPL was recognized via TLR2 not TLR4.
These results make a new avenue of the diagnosis and protection in non-tuberculous infection.
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| Academic Significance and Societal Importance of the Research Achievements |
難治性の呼吸器感染症である非結核性抗酸菌症の感染性・病原性を細胞表層に存在する糖ペプチド脂質抗原を中心に検討した。脂質生化学的、脂質免疫学的な基礎研究から非結核性抗酸菌症における有益な知見を得た。今後、診断や治療、予防への応用が期待できる。
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Report
(5 results)
Research Products
(32 results)
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[Journal Article] Naturally occurring a loss of a giant plasmid from Mycobacterium ulcerans subsp. shinshuense makes it non-pathogenic.2018
Author(s)
Nakanaga K, Ogura Y, Toyoda A, Yoshida M, Fukano H, Fujiwara N, Miyamoto Y, Nakata N, Kazumi Y, Maeda S, Ooka T, Goto M, Tanigawa K, Mitarai S, Suzuki K, Ishii N, Ato M, Hayashi T, Hoshino Y.
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Journal Title
Sci Rep.
Volume: 29;8(1)
Issue: 1
Pages: 8218-8218
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Mycobacterium shigaense sp. nov., a slow-growing, scotochromogenic species, is a member of the Mycobacterium simiae complex2018
Author(s)
H. Fukano, M. Yoshida, Y. Kazumi, N. Fujiwara, K. Katayama, Y. Ogura, T. Hayashi, Y. Miyamoto, N. Fujimoto, W. Hongsheng, C. Mizumoto, Y. Koizumi, H. Maeda, O. Hiranuma, S. Mitarai, N. Ishii, and Y. Hoshino
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Journal Title
Int J Syst Evol Microbiol.
Volume: 68(8)
Pages: 2437-2442
DOI
Related Report
Peer Reviewed
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[Journal Article] Microarray analysis of macrophage response to infection with Streptococcus oralis reveals the immunosuppressive effect of hydrogen peroxide2017
Author(s)
H. Matsushima, Y. Kumagai, A. Vandenbon, H. Kataoka, M. Kadena, H. Fukamachi, T. Arimoto, H. Morisaki, N. Fujiwara, N. Okahashi, and H. Kuwata
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Journal Title
Biochem Biophys Res Commun
Volume: 485(2)
Pages: 461-467
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Identification of a desaturase involved in mycolic acid biosynthesis in Mycobacterium smegmatis2016
Author(s)
A. Singh, C Varela, K. Bhatt, N. Veerapen, O.Y. Lee, H.H. Wu, G.S. Besra, D.E. Minnikin, N. Fujiwara, K. Teramoto, A. Bhatt
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Journal Title
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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