Non-canonical signaling networks mediated by an orphan two-component system histidine kinase in Vibrio cholerae

• Project/Area Number: 16K08798
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bacteriology (including mycology)
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: Shouji Yamamoto 国立感染症研究所, 細菌第一部, 主任研究官 (80469957)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2017: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 二成分制御系 / オルファンヒスチジンキナーゼ / 非二成分制御系 / PTS / シグナル伝達ネットワーク / コレラ菌 / キチン / シグナル伝達系 / PTS系 / シグナルネットワーク / DNA取り込み能 / キチン利用能 / 環境応答

Outline of Final Research Achievements

This study was done to understand how phosphoenolpyruvate:phosphotransferase system (PTS) interferes with chitin-activated signaling pathways initiated by the orphan two-component system histidine kinase ChiS in Vibrio cholerae. The results indicated that: 1) dephosphorylated EIIAglc of the PTS inhibits ChiS-mediated signaling, 2) this inhibition is independent of classical catabolite repression that is caused by lowered levels of cyclic AMP.

Academic Significance and Societal Importance of the Research Achievements

本研究では，キチンの応答に関わる二成分制御系と一般的な糖の取り込みに関わるPTS制御系という異なるタイプのシグナル伝達系が相互作用し，PTS側が二成分制御系側に干渉することを明らかにした。この成果は，細菌におけるシグナル伝達ネットワークの形成様式について新しい知見を提供するとともに，コレラ菌の感染を制御する技術の発展にも繋がることが期待される。

Research Products

• [Journal Article] Single Circular Chromosome Identified from the Genome Sequence of the Vibrio cholerae O1 bv. El Tor Ogawa Strain V060002. (2018)
  • Author(s): Yamamoto S, Lee KI, Morita M, Arakawa E, Izumiya H, Ohnishi M
  • Journal Title: Genome Announcements Volume: 6 Issue: 25
  • DOI: 10.1128/genomea.00564-18
  • Peer Reviewed / Open Access
• [Journal Article] Molecular mechanisms of Streptococcus pneumoniae-targeted autophagy via pneumolysin, Golgi-resident Rab41, and Nedd4-1-mediated K63-linked ubiquitination (2018)
  • Author(s): Ogawa M, Matsuda R, Takada N, Tomokiyo M, Yamamoto S, Shizukusihi S, Yamaji T, Yoshikawa Y, Yoshida M, Tanida I, Koike M, Murai M, Morita H, Takeyama H, Ryo A, Guan JL, Yamamoto M, Inoue JI, Yanagawa T, Fukuda M, Kawabe H, Ohnishi M
  • Journal Title: Cellular Microbiology Volume: e12846 Issue: 8
  • DOI: 10.1111/cmi.12846
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Glucose-Specific Enzyme IIA of the Phosphoenolpyruvate:Carbohydrate Phosphotransferase System Modulates Chitin Signaling Pathways in Vibrio cholerae (2017)
  • Author(s): Yamamoto S, Ohnishi M
  • Journal Title: Journal of Bacteriology Volume: 199 Issue: 18
  • DOI: 10.1128/jb.00127-17
  • Peer Reviewed / Open Access
• [Presentation] Regulation of natural competence by PTS in Vibrio cholerae (2017)
  • Author(s): Shouji Yamamoto
  • Organizer: The 90th Annual Meeting of Japanese Society for Bacteriology
  • Place of Presentation: 仙台
  • Year and Date: 2017-03-19
• [Remarks] Vibrio cholerae V060002 DNA, complete genome
  • URL: https://www.ncbi.nlm.nih.gov/nuccore/AP018677.1