Identification and functional analysis of host factors involved in early steps of hepatitis E virus infection
| Project/Area Number |
16K08817
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | Jichi Medical University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | E型肝炎ウイルス / 感染初期過程 / 受容体 / 細胞内侵入 / 感染感受性 / エクソソーム / エンベロープ / 細胞侵入 / マイクロアレイ / 細胞内輸送 |
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| Outline of Final Research Achievements |
In this study, to identify the possible entry receptors for hepatitis E virus (HEV) infection, we investigated the antigenicity and morphological features of the membrane-associated HEV particles. Our results indicated that membrane components are common between the membrane-associated HEV particles and exosomes, and that the capsids of HEV particles are individually covered by lipid membranes. In addition, our results suggested that the two candidate proteins identified in our previous study could serve as different entry receptors for two distinct forms of HEV particles.
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| Academic Significance and Societal Importance of the Research Achievements |
これまでの解析からHEVの感染受容体としての関与が示唆されている候補蛋白質に対して、siRNAを用いたスクリーニングを行った。その結果、どちらか一方の粒子形態でのみ増殖効率が低下する蛋白質を同定することができた。また、感染阻害実験やウイルスとの結合実験により、これらの蛋白質がHEVの感染受容体として機能することを示すことができた。感染受容体はHEVの感染初期過程に非常に重要な分子であるため、両者の結合を阻害するような化合物を探索することで、治療薬開発への基礎的知見を提供できるものと期待される。
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Report
(4 results)
Research Products
(18 results)
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[Journal Article] Full-length genome of a novel genotype 3 hepatitis E virus strain obtained from domestic pigs in Japan2017
Author(s)
Primadharsini PP, Miyake M, Kunita S, Nishizawa T, Takahashi M, Nagashima S, Tanggis, Ohnishi H, Kobayashi T, Nishiyama T, Jirintai S, Okamoto H
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Journal Title
Virus Research
Volume: 240
Pages: 147-153
DOI
Related Report
Peer Reviewed
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[Journal Article] Clinical and virological features of acute hepatitis E in Gunma prefecture, Japan between 2004 and 2015.2017
Author(s)
Yamazaki Y, Naganuma A, Arai Y, Takeuchi S, Kobayashi T, Takakusagi S, Hatanaka T, Hoshino T, Namikawa M, Hashizume H, Takizawa D, Ohyama T, Suzuki H, Horiguchi N, Takagi H, Sato K, Kakizaki S, Kusano M, Nagashima S, Takahashi M, Okamoto H, Yamada M.
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Journal Title
Hepatology Research
Volume: 47
Pages: 435-445
DOI
Related Report
Peer Reviewed
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