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The development of simple Amyloid beta detection method with aggregation induced emission dye labeled peptide

Research Project

Project/Area Number 16K08952
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Laboratory medicine
Research InstitutionKonan University

Principal Investigator

Murashima Takashi  甲南大学, フロンティアサイエンス学部, 教授 (20263923)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords凝集誘起発光色素 / アルツハイマー / アミロイドβ / 水溶性 / ピリジンN-オキシド / バックグラウンド蛍光 / 凝集誘起発光 / 親水性 / ピリジン-N-オキシド / テロメア / 立体障害 / アミロイド
Outline of Final Research Achievements

A novel, facile amyloid beta (Aβ) peptide detection method was developed based on an aggregation-induced emission (AIE) dye using a modified amyloid fibrillization-promoting peptide (AFPP) probe. The AFPP portion of the probe plays a role in accelerating Aβ fibrillization, and the AIE portion of the probe plays a role in signal emission. Since the probe molecules were uniformly dispersed in buffer solution without Aβ, the fluorescent intensity was weak. While Aβ was added to the probe solution, the aggregation of Aβ was promoted and the fluorescence was enhanced within 3 hrs. The limit of detection with this method is 4.2 nM. The limit of detection value is insufficient for the quantification of Aβ found in bodily fluids; however, the present method has the advantage of a simple “add-and-measure” procedure without the need for a high level of laboratory skills and sophisticated equipment.

Academic Significance and Societal Importance of the Research Achievements

高齢化が進む社会においてアルツハイマー病(AD)は大きな社会問題となっている。現在のところ、この疾患については根本的な治療法はないが、コリンエステラーゼ阻害剤などの、進行を遅らせるのに一定の効果を発揮する薬剤は開発されている。したがって、ADに対する対策として早期発見が非常に重要である。早期発見のためには、AD発症の数年以上前から増加することがわかっているアミロイドβの高感度検出が有効であり、本研究で開発した手法はその目的に適したものであることから社会的意義は高いと思われる。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (14 results)

All 2019 2018 2017 2016 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results,  Acknowledgement Compliant: 2 results) Presentation (11 results) (of which Int'l Joint Research: 2 results) Remarks (1 results)

  • [Journal Article] Nicotine-Selective Polymeric Adsorbent Obtained by Molecular Imprinting with Excess Use of Itaconic Acid2017

    • Author(s)
      野上晴加、中堀祐馬、村嶋貴之、松井淳
    • Journal Title

      CHROMATOGRAPHY

      Volume: 38 Issue: 1 Pages: 15-21

    • DOI

      10.15583/jpchrom.2016.017

    • NAID

      130005439674

    • ISSN
      1342-8284, 1348-3315
    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Elucidation of a “Signal-ON” Mechanism of Aggregation-Induced Emission Dye-Labelled DNA/DNA Duplexes and Application of a Repeat DNA Detection Method2016

    • Author(s)
      村嶋貴之、河村浩司、松本亜衣
    • Journal Title

      Medicinal Chemistry

      Volume: 6 Pages: 704-709

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] アゾ基を含む新規 AIE 色素の合成および物性評価2019

    • Author(s)
      村嶋貴之、立岩雅大
    • Organizer
      日本化学会第99春季年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] AIE 色素を用いた SNPs の特定方法2019

    • Author(s)
      村嶋貴之、衣笠紫野
    • Organizer
      日本化学会第99春季年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 複素環式芳香族化合物の N-Oxide をもつ新規 AIE 色素の合成とその物性評価2019

    • Author(s)
      島田直昭、村嶋貴之
    • Organizer
      日本化学会第99春季年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Synthesis of the AIE dye-DNA conjugate and the study on application possibility for biosensing2019

    • Author(s)
      村嶋貴之、谷 知輝
    • Organizer
      日本化学会第99春季年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Pyridine N-Oxide構造を持つ新規AIE色素の合成とその物性評価2018

    • Author(s)
      島田直昭、村嶋貴之
    • Organizer
      日本化学会第98春季年会
    • Related Report
      2017 Research-status Report
  • [Presentation] 親水性AIE色素を用いた新規シグナルオン型プローブの開発2018

    • Author(s)
      高木武宗、村嶋貴之
    • Organizer
      日本化学会第98春季年会
    • Related Report
      2017 Research-status Report
  • [Presentation] AIE色素とDNAのコンジュゲート作成及びバイオプローブへの応用2018

    • Author(s)
      村嶋貴之、谷 知輝
    • Organizer
      日本化学会第98春季年会
    • Related Report
      2017 Research-status Report
  • [Presentation] Facile Quantification of Alzheimer’s Disease Amyloid-β based on Aggregation-Induced Emission.2017

    • Author(s)
      星川喬哉、村嶋貴之
    • Organizer
      32nd International Conference of Alzheimer’s Disease International
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] 凝集有機発光色素を用いたAβ簡易検出法の開発2017

    • Author(s)
      星川喬哉、村嶋貴之
    • Organizer
      日本化学会 第97春季年会
    • Place of Presentation
      慶應義塾大学日吉キャンパス(神奈川県、横浜市)
    • Related Report
      2016 Research-status Report
  • [Presentation] Synthesis of High-affinity Nicotine-imprinted Polymer by Highly Excess Usage of Itaconic Acid as Functional Monomer2016

    • Author(s)
      野上晴加、中堀祐馬、村嶋貴之、松井淳
    • Organizer
      The 9th International Conference on Molecular Imprinting (MIP2016)
    • Place of Presentation
      スェーデン、ルンド
    • Year and Date
      2016-06-25
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] TICT型BODIPYを用いたナノ粒子の作製2016

    • Author(s)
      松本亜衣、河村浩司、村嶋貴之
    • Organizer
      第6回CSJ化学フェスタ
    • Place of Presentation
      タワーホール船堀(東京都、江戸川区)
    • Related Report
      2016 Research-status Report
  • [Remarks] 有機合成化学研究室

    • URL

      http://www.pi.konan-u.ac.jp/murashima/index.html

    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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