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Analysis on the genotype transition of measles virus prevailing in Japan - molecular bases explaining the ability of the virus to spread in the field

Research Project

Project/Area Number 16K09112
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Hygiene and public health
Research InstitutionNagahama Institute of Bio-Science and Technology

Principal Investigator

ITOH MASAE  長浜バイオ大学, バイオサイエンス学部, 教授 (10201328)

Research Collaborator Takahashi Ken-ichi  
Wakimoto Hiroshi  
Satoh Yuto  
Sakamoto Kento  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords麻疹ウイルス / 遺伝子型 / 流行株 / 個体間伝搬 / 個体間伝播 / ウイルス排出 / 麻疹 / ウイルス / ゲノム / 感染症 / 伝播
Outline of Final Research Achievements

Measles virus (MV) is genetically highly variable and classified into 24 genotypes, whose comparison among genotypes has hardly been performed so far. Here, the growth characteristics were studied on the genotype D3- and H1-MV that caused outbreak in Japan in the late 1990s and after 2000, respectively. The growth pattern of the H1-MV absolutely differed from that of the D3-MV. The H protein of the H1-MV enhanced, but the F protein reduced the cell fusion as well as the infectious progeny virus production compared to those of the D3-MV. The results demonstrated for the first time that functional activities of MV proteins differ, leading to the specific growth characteristics depending on the genotypes. Transition of the MV genotype prevailing in an endemic area could not be explained by the antigenicity of the virus and immunized status of the residents since MV is thought to be serologically monotypic. Difference in growth characteristics might be possible to cause the transition.

Academic Significance and Societal Importance of the Research Achievements

麻疹ウイルスは、A遺伝子型のワクチンが野生株の全ての遺伝子型に対して有効で遺伝子型間で抗原性に大差がなく、遺伝子型特異的抗原性や血中抗体の保有状況など従来の疫学因子では日本国内で流行株が置換した現象を説明できない。これまで遺伝子型間で、増殖特性の比較はほとんど行われてこなかったが、本研究では、D3型とH1型で大きく異なること、ウイルス蛋白質の機能も遺伝子型で違いのあることを明らかにした。遺伝子型間でのこの違いが流行株交代を説明する可能性があり、その意義は大きい。D3型とH1型の増殖特性を決定するウイルス蛋白質の同定と機序の解明には至らず今後の課題である。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report

Research Products

(9 results)

All 2018 2017 2016

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Acknowledgement Compliant: 1 results,  Open Access: 1 results) Presentation (6 results)

  • [Journal Article] Nonstructural protein of severe fever with thrombocytopenia syndrome phlebovirus targets STAT2 and not STAT1 to inhibit type I interferon-stimulated JAK-STAT signaling.2018

    • Author(s)
      Yoshinori Kitagawa, Madoka Sakai, Masayuki Shimojima, Masayuki Saijo, Masae Itoh, Bin Gotoh.
    • Journal Title

      Microbes and Infection

      Volume: 20 Pages: 360-368

    • DOI

      10.1016/j.micinf.2018.05.007

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A residue located at the junction of the head and stalk regions of measles virus fusion protein regulates membrane fusion by controlling conformational stability2017

    • Author(s)
      Yuto Satoh, Saeka Yonemori, Mitsuhiro Hirose, Hiroko Shogaki, Hiroshi Wakimoto, Yoshinori Kitagawa, Bin Gotoh, Tsuyoshi Shirai, Ken-ichi Takahashi, and Masae Itoh
    • Journal Title

      Journal of General Virology.

      Volume: 98 Pages: 143-154

    • DOI

      10.1099/jgv.0.000670

    • Related Report
      2017 Research-status Report 2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Human Metapneumovirus M2-2 Protein Acts as a Negative Regulator of Alpha Interferon Production by Plasmacytoid Dendritic Cells2017

    • Author(s)
      Kitagawa Yoshinori、Sakai Madoka、Funayama Mariko、Itoh Masae、Gotoh Bin
    • Journal Title

      Journal of Virology

      Volume: 91

    • DOI

      10.1128/jvi.00579-17

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Mutation in the matrix (M) protein is the first requirement for adaptation of the wild-type measles virus to Vero cells.2018

    • Author(s)
      Miyuki Tsunemi, Akihiko Shinoda, Yuto Satoh, Kento Sakamoto, Hiroshi Wakimoto, Miho Konami, Takumi Tsukamoto, Hiroko Shogaki, Yoshinori Kitagawa, Bin Gotoh, Ken-ichi Takahashi, Masae Itoh.
    • Organizer
      The 66th Annual Meeting of the Japanese Society for Virology
    • Related Report
      2018 Annual Research Report
  • [Presentation] Middle East respiratory syndrome coronavirus accessory proteins that can block TLR7/9-dependent signaling leading to interferon production.2018

    • Author(s)
      Yoshinori Kitagawa, Takumi Tsukamoto, Miki Kohno, Masae Itoh, Bin Gotoh.
    • Organizer
      The 66th Annual Meeting of the Japanese Society for Virology
    • Related Report
      2018 Annual Research Report
  • [Presentation] Measles virus fusion protein carries leucine zipper-like conformation optimized for efficient fusion induction in the HR-B domain2017

    • Author(s)
      Shogaki Hiroko、Satoh Yuto、Wakimoto Hiroshi、Kitagawa Yoshinori、Gotoh Bin、Takahashi Ken-Ichi、Itoh Masae
    • Organizer
      The 65th Annual Meeting of the Japanese Society for Virology
    • Related Report
      2017 Research-status Report
  • [Presentation] Effect of human metapneumovirus M2-2 on Toll-like receptor 7/9-dependent K63-linked polyubiquitination and phosphorylation of IRF72017

    • Author(s)
      Kitagawa Yoshinori、Kohno Miki、Tsukamoto Takumi、Itoh Masae、Gotoh Bin
    • Organizer
      The 65th Annual Meeting of the Japanese Society for Virology
    • Related Report
      2017 Research-status Report
  • [Presentation] Functional roles of the hemagglutinin protein of a subacute sclerosing panencephalitis (SSPE) virus, the Kobe-1 strain.2016

    • Author(s)
      Yuto Satoh, Daichi Nishikawa, Kurara, Higuchi, Da-Peng Jiang, Hiroko Shogaki, Hiroshi Wakimoto, Yoshinori Kitagawa, Bin Gotoh, Ken-ichi Takahashi, Hak Hotta, Masae Itoh.
    • Organizer
      The 64th Annual Meeting of the Japanese Society for Virology.
    • Place of Presentation
      Sapporo Convention Center(北海道札幌市)
    • Year and Date
      2016-10-23
    • Related Report
      2016 Research-status Report
  • [Presentation] Effect of human metapneumovirus M2-2 protein expression on IRF7 homodimerization critical for alpha interferon gene activation.2016

    • Author(s)
      Yoshinori Kitagawa, Mariko Funayama, Madoka Sakai, Masae Itoh, Bin Gotoh.
    • Organizer
      The 64th Annual Meeting of the Japanese Society for Virology.
    • Place of Presentation
      Sapporo Convention Center(北海道札幌市)
    • Year and Date
      2016-10-23
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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