| Project/Area Number |
16K10428
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | The University of Tokushima |
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Principal Investigator |
MORINE Yuji 徳島大学, 大学院医歯薬学研究部(医学域), 准教授 (60398021)
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| Co-Investigator(Kenkyū-buntansha) |
池本 哲也 徳島大学, 病院, 特任准教授 (20398019)
齋藤 裕 徳島大学, 病院, 特任助教 (50548675)
居村 暁 徳島大学, 病院, 特任教授 (90380021)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 異所性肝移植 / ADRC / 胆管細胞 / 3次元培養 / 肝細胞 / 肝再生 / 胆管構築 |
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| Outline of Final Research Achievements |
The purpose of this study was to establish the procedure of functional liver tissue rebuilding with bile duct structure on heterotopic liver tissue transplantation using adipose-derived regenerative cells (ADRC) and 3D culture system.
We demonstrated the protective effect of ADRC for transplant liver function and the differentiation to hepatocyte like cell, but not the ability to differentiate along multiple lineage pathways for other liver component cell from ADRC. Therefore, the pre-induction protocol of ADRC towards cholangiocyte seemed to be necessary before the combined transplantation. Also, 3D culture system indicated the protective effect of primary hepatocyte culture. But, we could not establish the optimal protocol of cholangiocyte differentiation from ADRC, yet. Consequently, while there were some future problems, the possibility of 3D culture system on liver tissue transplantation and the directionality of liver tissue rebuilding approach could be indicated in this study.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究ではADRC・3次元培養システムを活用した異所性肝移植の長期安定化や胆管構築を伴った機能的異所性肝組織再構築の可能性について検討した。残念ながら、ADRCの肝細胞機能維持は確認されたが、胆管細胞分化・組織再構築までの結果を得ることはできず、胆管構造を有する組織再構築には、課題が残ったが、3次元培養システムの可能性や肝組織再構築手法の方向性を示すことはできたと思われる。
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