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Effective differentiation of insulin-producing cell from ADSC with three dimensional culture method

Research Project

Project/Area Number 16K10429
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field General surgery
Research InstitutionThe University of Tokushima

Principal Investigator

IKEMOTO Tetsuya  徳島大学, 病院, 特任准教授 (20398019)

Co-Investigator(Kenkyū-buntansha) 齋藤 裕  徳島大学, 病院, 特任助教 (50548675)
森根 裕二  徳島大学, 大学院医歯薬学研究部(医学域), 准教授 (60398021)
居村 暁  徳島大学, 病院, 特任教授 (90380021)
Research Collaborator YAMADA Shinichiro  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsインスリン産生細胞 / 脂肪由来幹細胞 / 3次元培養 / RCPピース / Insulin-producing cell / ADSC / 幹細胞分化 / 膵島移植 / 1型糖尿病 / I型糖尿病 / IPC / β細胞補填療法
Outline of Final Research Achievements

Generation of insulin-producing cells (IPCs) is expected as the new option for the radical therapy of type 1 DM patients. Technique of regenerative medicine is considered as useful for generating these IPCs from mesenchymal stem cells. Among these cells, we have focused on adipose-derived stem cells (ADSCs) due to its multipotency and easy-procurement, thus we have already established an easy and rapid 2-step differentiation protocol for IPCs from ADSC. However, some issues which should be resolved still remain such as the poor cell expansion rate and the lot equalization of generated IPCs. For solving these issues, here we are investigating to establish a rapid and more effective protocol of generating IPCs from ADSCs with choosing the best procedure among three dimensional culture systems which are many reported, by modifying our 2-step protocol.

Academic Significance and Societal Importance of the Research Achievements

ADSCは、採取量が豊富・倫理的問題が少ない・遺伝子導入などの DNA ダメージがない、といった有用性のために臨床応用が比較的容易である。上記の実験結果から得られる知見は、安全性が担保され、脳死アロ膵島移植と同等もしくはそれ以上の成績であることが実証されれば、即臨床応用可能である。今後、donor source の問題解決のみならず、膵島移植自体へのブレイクスルーとなる可能性を秘めている。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (11 results)

All 2019 2018 2017 2016

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (8 results)

  • [Journal Article] The protective effect of epigallocatechin 3-gallate on mouse pancreatic islets via the Nrf2 pathway2019

    • Author(s)
      Wada Yuma、Takata Atsushi、Ikemoto Tetsuya、Morine Yuji、Imura Satoru、Iwahashi Shuichi、Saito Yu、Shimada Mitsuo
    • Journal Title

      Surgery Today

      Volume: Epub ahead of print Issue: 6 Pages: 536-545

    • DOI

      10.1007/s00595-019-1761-0

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of monoclonal mouse antibodies that specifically recognize pancreatic polypeptide2019

    • Author(s)
      Hara A, Nakagawa Y, Nakao K, Tamaki M, Ikemoto T, Shimada M, Matsuhisa M, Mizukami H, Maruyama N, Watada H, Fujitani Y.
    • Journal Title

      Endocrine Journal

      Volume: 66 Issue: 5 Pages: 459-468

    • DOI

      10.1507/endocrj.EJ18-0441

    • NAID

      130007655243

    • ISSN
      0918-8959, 1348-4540
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A New 2-Step Acceleration Protocol Using a Histone Deacetylase Inhibitor to Generate Insulin-Producing Cells From Adipose-Derived Mesenchymal Stem Cells2018

    • Author(s)
      Ikemoto Tetsuya、Feng Rui、Shimada Mitsuo、Saito Yu、Iwahashi Shuichi、Morine Yuji、Imura Satoru
    • Journal Title

      Pancreas

      Volume: 47(4) Issue: 4 Pages: 477-481

    • DOI

      10.1097/mpa.0000000000001017

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 脂肪由来幹細胞からのインスリン産生細胞分化誘導に関する研究2019

    • Author(s)
      1.和田佑馬, 池本哲也, 居村暁, 岩橋衆一, 齋藤裕, 山田眞一郎, 太田昇吾, 岩橋祥子, 島田光生
    • Organizer
      第18回日本再生医療学会総会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Insulin-producing cell(IPC)分化成熟と亜鉛イオン濃度相関に関する研究2019

    • Author(s)
      2.太田昇吾, 島田光生, 池本哲也, 森根裕二, 居村暁, 岩橋衆一, 齋藤裕, 山田眞一郎, 和田佑馬, 良元俊昭, 岩橋祥子
    • Organizer
      第18回日本再生医療学会総会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 臨床応用を目指した間葉系幹細胞から創生されるインスリン産生細胞による糖尿病の治療法確立に関する研究2018

    • Author(s)
      1.池本哲也, 島田光生, 居村暁, 森根裕二, 岩橋衆一, 齋藤裕, 和田佑馬, 太田昇吾
    • Organizer
      第54回日本移植学会総会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 臨床応用を目指した間葉系幹細胞から創生されるインスリン産生細胞による糖尿病の治療法確立に関する研究2018

    • Author(s)
      池本哲也, 島田光生, 森根裕二, 居村暁, 岩橋衆一, 齋藤裕, 和田佑馬, 寺奥大貴, 太田昇吾
    • Organizer
      第118回日本外科学会定期学術集会
    • Related Report
      2017 Research-status Report
  • [Presentation] 膵島移植に対するブレイクスルーとしてのAdipose tissue derived stem cellsからの効果的Insulin-producing cells創生2017

    • Author(s)
      池本哲也, 島田光生, 居村暁, 森根裕二, 岩橋衆一, 齋藤裕, 寺奥大貴
    • Organizer
      第53回日本移植学会総会
    • Related Report
      2017 Research-status Report
  • [Presentation] The establishment of effective differentiation protocols for insulin-producing cells using 3D culture system from rat adipose-tissue derived mesenchymal stem cell2017

    • Author(s)
      Ikemoto T, Shimada M, Iwahashi S,Rui Feng, Yoshimoto T, Saito Y, Higashijima J, Morine Y. and Imura S.
    • Organizer
      European Society for Organ Transplantation
    • Related Report
      2017 Research-status Report
  • [Presentation] 脂肪由来幹細胞によるInsulin-producing cell作成における新規分化誘導方法に関する検討2016

    • Author(s)
      1.池本哲也, 島田光生, 居村暁, 森根裕二, 岩橋衆一, 齋藤裕, 吉川雅登, 良元俊昭
    • Organizer
      第52回日本移植学会総会
    • Place of Presentation
      グランドプリンスホテル新高輪(東京都港区)
    • Year and Date
      2016-10-01
    • Related Report
      2016 Research-status Report
  • [Presentation] The prospects and challenges of achievement in islet transplantation using adipose-tissue derived mesenchymal stem cell2016

    • Author(s)
      池本 哲也
    • Organizer
      第71回日本消化器外科学会総会
    • Place of Presentation
      あわぎんホール(徳島県徳島市)
    • Year and Date
      2016-07-16
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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