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Investigation of extracellular vesicles as novel biomarker in 4 type gastric cancer patients

Research Project

Project/Area Number 16K10521
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Digestive surgery
Research InstitutionOsaka Medical College

Principal Investigator

Lee Sang-Woong  大阪医科大学, 医学部, 講師 (40368080)

Co-Investigator(Kenkyū-buntansha) 赤尾 幸博  岐阜大学, 大学院連合創薬医療情報研究科, 特任教授 (00222505)
田代 圭太郎  大阪医科大学, 医学部, 講師 (20645527)
河合 英  大阪医科大学, 医学部, 講師 (40465604)
内山 和久  大阪医科大学, 医学部, 教授 (80232867)
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords胃癌 / 胃液 / 細胞外小胞 / Extracellular Vesicles / Exosome / Microvesicles / 微小環境 / EVs / エクソソーム / マイクロベジクル / 超遠心法 / 医療・福祉 / 4型胃癌 / バイオマーカー
Outline of Final Research Achievements

In this study, we sought to clarify the existence of GJ-EVs derived from gastric cancer patients. GJ-EVs were isolated by the ultracentrifuge method combined with our own preprocessing from gastric cancer (GC) patients. We verified GJ-EVs by morphological experiments, i.e., nanoparticle tracking system analysis and electron microscopy. In addition, protein and microRNA markers of EVs were examined by Western blotting analysis, Bioanalyzer, or quantitative reverse transcription polymerase chain reaction. GJ-EVs were found to promote the proliferation of normal fibroblast cells. Our findings suggest that isolates from the GJ of GC patients contain EVs and imply that GJ-EVs partially affect their microenvironments and that analysis using GJ-EVs from GC patients will help to clarify the pathophysiology of GC.

Academic Significance and Societal Importance of the Research Achievements

がん患者の血液や尿など様々な体液中の細胞外小胞に対する解析がなされているが、本研究では、粘調度によりこれまで抽出が試みられてこなかった胃液中にも細胞外小胞が含有されることが同定された。また、がん細胞から放出されるであろう胃液中の細胞外小胞が周囲の微小環境に影響を与えることが示唆された。本研究成果により、がんにおける細胞外小胞の役割がより付加されたと考える。また本研究は、胃癌患者の胃液という臨床試料を用いた検討でありより臨床を反映した研究成果であると考える。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (4 results)

All 2019 2018 2017 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (2 results) Remarks (1 results)

  • [Journal Article] Analysis of Extracellular Vesicles in Gastric Juice from Gastric Cancer Patients2019

    • Author(s)
      Kagota Shuji、Taniguchi Kohei、Lee Sang-Woong、Ito Yuko、Kuranaga Yuki、Hashiguchi Yasuyuki、Inomata Yosuke、Imai Yoshiro、Tanaka Ryo、Tashiro Keitaro、Kawai Masaru、Akao Yukihiro、Uchiyama Kazuhisa
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 20 Issue: 4 Pages: 953-953

    • DOI

      10.3390/ijms20040953

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 胃液由来細胞外小胞の抽出と機能解析2018

    • Author(s)
      駕田修史、谷口高平、猪俣陽介、倉永祐希、伊藤裕子、赤尾幸博、内山和久
    • Organizer
      第77回 日本癌学会学術総会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Extraction and functional analysis of extracellular vesicles derived from gastric juice2017

    • Author(s)
      Shuji Kagota
    • Organizer
      第21回日本がん免疫学会総会
    • Related Report
      2017 Research-status Report
  • [Remarks] 大阪医科大学 一般・消化器外科学教室 研究室

    • URL

      https://www.osaka-med.ac.jp/~sur000/html/laboratory.html

    • Related Report
      2018 Annual Research Report

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Published: 2016-04-21   Modified: 2020-03-30  

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