Identification of molecular mechanism of mechanobiological bone formation around implant

• Project/Area Number: 16K11582
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Prosthodontics/ Dental materials science and
• Research Institution: Tohoku University
• Principal Investigator: Matsui Hiroyuki 東北大学, 東北メディカル・メガバンク機構, 講師 (10547277)
• Co-Investigator(Kenkyū-buntansha): 佐々木 啓一 東北大学, 歯学研究科, 教授 (30178644)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: メカノバイオロジー / ジルコニア / 生体親和性 / ナノジルコニア / メカニカルストレス / ERK / 骨メカノバイオロジー

Outline of Final Research Achievements

In this study, we aimed to identify the relation between cell morphology and biocompatibility on zirconia surface from mechanobiological perspectives. Osteobalats showed sharp spindle shape on both yttria-stabilized zirconia (Y-TZP) and seria-stabilized zirconia (NANOZR). This shape form was inhibited both by integrin inhibition and heparin treatment, however, cellular reaction was different. Briefly, integrin alpah2beta1 negatively regulates proliferation only on Y-TZP, but heparin-sensitive protein positively regulates proliferation on NANOZR. These results suggest that osteobalst possesses multiple specific adhesion molecule against specific substrate, however, they integrate cellular context to proliferation by unknown mechanism.

Academic Significance and Societal Importance of the Research Achievements

ジルコニアはアレルギーがなく強度に優れており，金属に代わる歯科用材料として注目されている．特に近年，CAD/CAMによる加工技術の進歩が臨床応用の幅を広げている．このことは同時に，ジルコニア材料の改質や新たな表面修飾を有するジルコニアの開発の必要性を示している．一方，ジルコニアの生体親和性の分子メカニズムはほとんど不明である．本研究はこれをつぶさに解明し，新材料開発の礎を築くことに意義がある．

Research Products

• [Journal Article] Initial osteoblast adhesion and subsequent differentiation on zirconia surfaces are regulated by integrins and heparin-sensitive molecule. (2018)
  • Author(s): Luo F, Hong G, Matsui H, Endo K, Wan Q, Sasaki K
  • Journal Title: Int J Nanomedicine Volume: 13 Pages: 7657-7667
  • DOI: 10.2147/ijn.s175536
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Heparin-sensitive molecule regulates osteoblast adhesion and proliferation on zirconia surface (2018)
  • Author(s): Luo F., Hong G., Matsui H., Endo K. and Sasaki K.
  • Organizer: European association of osseointegration
  • Int'l Joint Research
• [Presentation] Effect of RGD Peptides on Cellular Morphology and Adhesion (2017)
  • Author(s): F. Luo, G. Hong, H. Matsui, K. Endo, Q. Wan, and K. Sasaki
  • Organizer: International association of dental research
  • Int'l Joint Research
• [Presentation] Identification of MAP3K of ERK pathway activated by low-magnitude of mechanical stress in osteoblasts (2016)
  • Author(s): Hiroyuki Matsui, Qi Zhang, Xing Liang, Keiichi Sasaki
  • Organizer: European association of osseointegration
  • Place of Presentation: Palais des congress, Paris, France
  • Year and Date: 2016-09-29
  • Int'l Joint Research