Effect of brain-derived neurotrophic factor (BDNF) on macrophage activation for periodontal therapy

• Project/Area Number: 16K11831
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Periodontology
• Research Institution: Hiroshima University
• Principal Investigator: Takeda Katsuhiro 広島大学, 医歯薬保健学研究科(歯), 助教 (10452591)
• Research Collaborator: Sasaki Shinya
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 脳由来神経栄養因子 / 歯周炎 / 非外科的歯周治療 / ビーグル犬

Outline of Final Research Achievements

In this study, we investigated the effect of scaling and root planning (SRP) with local application of BDNF/Hyaluronic acid(HMW-HA) complex on the changes in clinical parameters and histology of periodontal tissue in dogs with ligature-induced periodontitis. The BDNF/HMW-HA group showed significant improvement of clinical parameters compared to those of the other groups in dogs. Histological analysis on the BDNF/HMW-HA group indicated suppression of apical migration of epithelial tissue and milder infiltration of inflammatory cells than it was observed in the other three groups. Furthermore, new cementum and alveolar bone were regenerated and collagen fibers were inserted into them in the BDNF/HMW-HA group.

Academic Significance and Societal Importance of the Research Achievements

軽度歯周組織破壊は進行した歯周炎と比較すると、①重症化の危険性がある、②完全再生の可能性がある、③罹患患者が多いという特徴がある。BDNFによって軽度歯周組織破壊を非外科的に再生させることができれば、患者によるセルフケアが容易になり、歯周炎再発・重症化のリスクが軽減する。また、歯周炎は全身疾患と関連していることから、歯周炎を早期に治療することは全身的な疾患リスクの低減につながる。

Research Products

• [Journal Article] BDNF/HMW-HA complex as an adjunct to nonsurgical periodontal treatment of ligature-induced periodontitis in dogs (2018)
  • Author(s): Sasaki Shinya、Takeda Katsuhiro、Takewaki Manabu、Ouhara Kazuhisa、Kajiya Mikihito、Mizuno Noriyoshi、Fujita Tsuyoshi、Kurihara Hidemi
  • Journal Title: Journal of Periodontology Volume: 90 Issue: 1 Pages: 98-109
  • DOI: 10.1002/jper.18-0070
  • Peer Reviewed / Open Access
• [Presentation] 脳由来神経栄養因子（BDNF）がマクロファージ活性化に及ぼす影響 (2018)
  • Author(s): 佐々木慎也，武田克浩，應原一久，本池総太，加治屋幹人，松田真司，水野智仁，藤田 剛，栗原英見
  • Organizer: 第61回春季日本歯周病学会学術大会