Effect of reduced repair ability of DNA double strand beaks on cognitive function in neurons
| Project/Area Number |
16K12599
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Risk sciences of radiation and chemicals
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
Kodak Seiji 大阪府立大学, 理学(系)研究科(研究院), 教授 (00195744)
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| Co-Investigator(Renkei-kenkyūsha) |
SHIRAISHI Kazunori 大阪府立大学, 理学系研究科生物科学専攻, 助教 (40347513)
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| Research Collaborator |
KASHIWAGI Hiroki 大阪府立大学, 理学系研究科生物科学専攻, 客員研究員
SAKAGUCHI Kenta 大阪府立大学, 理学系研究科生物科学専攻, 客員研究員
OIE Ayaka 大阪府立大学, 理学系研究科生物科学専攻
KANABOSHI Sakura 大阪府立大学, 理学系研究科生物科学専攻
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | ニューロン / 神経幹/前駆細胞 / DNA２本鎖切断 / 非相同末端結合 / DNA依存的プロテインキナーゼ / アポトーシス / DNA２本鎖切断修復 / DNA2本鎖切断 / 神経系発生 / 損傷 / 神経系細胞 |
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| Outline of Final Research Achievements |
We investigated the repair kinetics of X-ray-induced DNA double-strand beaks (DSBs) in mouse neural stem/progenitor cells (NSPCs) and their differentiated neurons by scoring the number of phosphorylated histone H2AX (gamma-H2AX) foci or phosphorylated 53BP1 foci post-irradiation. The DSB repair in neurons differentiated from NSPCs in culture was faster than that in mouse embryonic fibroblasts (MEFs), possibly due to the higher DNA-dependent protein kinase activity, but similar to that in NSPCs. Further, the incidence of p53-dependent apoptosis induced by X-irradiation in neurons was significantly higher than that in NSPCs. This difference in response of X-ray-induced apoptosis between neurons and NSPCs may reflect the difference in the fidelity of non-homologous end joining or the differential sensitivity to DNA damage other than DSBs.
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Report
(3 results)
Research Products
(19 results)
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[Journal Article] A Cyclized Helix-Loop-Helix Peptide as a Molecular Scaffold for the Design of Inhibitors of Intracellular Protein-Protein Interactions by Epitope and Arginine Grafting2016
Author(s)
D. Fujiwara, H. Kitada, M. Oguri, T. Nishihara, M. Michigami, K. Shiraishi, E. Yuba, I. Nakase, H. Im, S. Cho, J. Y. Joung, S. Kodama, K. Kono, S. Ham, I. Fujii,
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Journal Title
Angew. Chem. Int. Ed
Volume: 55
Pages: 10612-10615
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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