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Effects of polyunsaturated fatty acids supplemented in medium on primary culture

Research Project

Project/Area Number 16K12864
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biomedical engineering/Biomaterial science and engineering
Research InstitutionYamagata University

Principal Investigator

NAKAMURA TAKAO  山形大学, 大学院医学系研究科, 教授 (00142654)

Co-Investigator(Renkei-kenkyūsha) KUSUNOKI Masataka  名古屋大学, 総合保健体育科学センター, 教授 (80214956)
SATO Daisuke  山形大学, 大学院医学系研究科, 助教 (60536960)
Research Collaborator FENG Zhonggang  
TAKAHASHI Kazuaki  
SASAKI Hiroyuki  
KOSAKA Kaoru  
IGUCHI Seiya  
SAITO Jo  
TAKAHASHI Shinya  
OTORI Maki  
YANO Mizuna  
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords再生医工学 / 脂質 / 初代培養 / 心筋細胞 / 脂肪細胞
Outline of Final Research Achievements

Primary culture of rat cardiomyocytes was conducted, using medium containing one of 5 fatty acids including docosahexaenoic (DHA) and arachidonic (AA) acids. Maximal contractile performance was obtained in cases of 20μM DHA and 50μA AA, with large difference in gene expression. Although combined supplementation of 10μM DHA and 10-20μM AA showed maximal performance of contractility, its level stayed rather close to that obtained in single DHA supplementation.
Primary culture of adult rat adipocytes was superior to that of juvenile one. However, even in the case of DHA supplementation, retaining of adipocytes remained difficult. These results may show effects of fatty acids are dependent on cell species.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (7 results)

All 2018 2017 2016

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (6 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results)

  • [Journal Article] Unknown biological effects of L-glucose, ALA, and PUFA2017

    • Author(s)
      Yamada, K., Sato, D., Nakamura, T., Amano, H., and Morimoto, Y.
    • Journal Title

      J. Physiol. Sci.

      Volume: 67 Issue: 5 Pages: 539-548

    • DOI

      10.1007/s12576-017-0544-x

    • NAID

      40021301945

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Supplementation of arachidonic or docosahexaenoic acid in culture medium improves contractile performance of cardiomyocytes2018

    • Author(s)
      Nakamura T, Sato D, Saito J, Feng Z, Takahashi K, Kusunoki M
    • Organizer
      World Cong Med Physics Biomed Eng
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 多価不飽和脂肪酸の培地添加は培養心筋細胞の拍動収縮能を改善する2018

    • Author(s)
      佐藤大介,大鳥真紀,馮忠剛,楠正隆,中村孝夫
    • Organizer
      第57回日本生体医工学会大会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] ラット初代培養心筋細胞の拍動能に及ぼすドコサヘキサエン酸及びアラキドン酸の効果2017

    • Author(s)
      斉藤丈,小坂薫,佐藤大介,馮忠剛,楠正隆,中村孝夫
    • Organizer
      第56回日本生体医工学会大会
    • Related Report
      2017 Annual Research Report 2016 Research-status Report
  • [Presentation] ラット培養心筋細胞の拍動能及び脂肪酸組成に及ぼす多価不飽和脂肪酸の効果2017

    • Author(s)
      佐藤大介,小坂薫,斉藤丈,楠正隆,中村孝夫
    • Organizer
      第69回日本生物工学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Polyunsaturated fatty acids to maintain cardiomyocyte function in culture2016

    • Author(s)
      Nakamura T
    • Organizer
      Bio-IST Symp
    • Place of Presentation
      Kuala Lumpur (Malaysia)
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 多価不飽和脂肪酸の培地添加がラット培養心筋細胞の拍動収縮能に及ぼす効果2016

    • Author(s)
      小坂薫,井口 聖也,佐藤大介,馮 忠剛,楠正隆,中村孝夫
    • Organizer
      SICE ライフエンジニアリング部門シンポジウム2016
    • Place of Presentation
      大阪国際交流センター(大阪)
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2019-03-29  

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