Development of Non-Enzymatic Method to Translate D-Nucleic Acid to L-Nucleic Acid
| Project/Area Number |
16K14028
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bio-related chemistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
Taniguchi Tohru 北海道大学, 先端生命科学研究院, 助教 (00587123)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 鏡像体 / 核酸 / 構造解析 / キラリティー / らせん / 有機化学 |
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| Outline of Final Research Achievements |
Aiming both at creation of mirror-image life and at development of nucleic acid drugs resistant to nucleases, this work studied toward creation of non-enzymatic reaction to translate D-nucleic acid to L-nucleic acid. During the synthesis of L-nucleotide monomers, we succeeded in developing an efficient glycosidation reaction to connect a sugar and a nucleobase. We also established a new method to elucidate the configuration and conformation of furanoside and nucleoside by using vibrational circular dichroism. L-Nucleotides obtained in this study were submitted to solid-state synthesis, which yielded several oligonucleotides containing L-nucleotides. Oligonucleotides containing L-methylcytidines showed high stability when forming a duplex with D-oligonucleotides.
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Report
(3 results)
Research Products
(5 results)
