| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
Aiming both at creation of mirror-image life and at development of nucleic acid drugs resistant to nucleases, this work studied toward creation of non-enzymatic reaction to translate D-nucleic acid to L-nucleic acid. During the synthesis of L-nucleotide monomers, we succeeded in developing an efficient glycosidation reaction to connect a sugar and a nucleobase. We also established a new method to elucidate the configuration and conformation of furanoside and nucleoside by using vibrational circular dichroism. L-Nucleotides obtained in this study were submitted to solid-state synthesis, which yielded several oligonucleotides containing L-nucleotides. Oligonucleotides containing L-methylcytidines showed high stability when forming a duplex with D-oligonucleotides.
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