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Development of induction method of insulin-producing cells in vivo

Research Project

Project/Area Number 16K14588
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Laboratory animal science
Research InstitutionUniversity of Tsukuba

Principal Investigator

TAKAHASHI Satoru  筑波大学, 医学医療系, 教授 (50271896)

Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsインシュリン / β細胞 / 再生 / 転写因子 / 肝細胞 / インスリン / 肝臓細胞
Outline of Final Research Achievements

To search for new factors that promote conversion to insulin-producing cells from liver cells, Mouse insulin promoter 1-GFP transgenic mouse (MIP-GFP Tg), which has GFP reporter regulated by mouse insulin promoter was used. After isolating insulin producing cells, we did microarray analysis for identifing genes that are expressed in normal β cells, but not in induced insulin producing cells. As a result, new candidate genes Isl1β and Efl3 were identified. Each candidate gene was examined to confirm cooperative action in insulin production with Pdx1, NeuroD, MafA in vivo. Coexpression of Isl1β enhanced insulin production from liver cells by about 10 times. On the other hand, coexpression of Efl3 revealed that insulin transcription was suppressed contrary to expectation.
From the above results, it has been confirmed that Isl1β, together with Pdx1, NeuroD, MafA, has an effect of enhancing the production of insulin from liver cells. These results were published in Endocrinology.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (5 results)

All 2017 2016 Other

All Journal Article (2 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 2 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Remarks (3 results)

  • [Journal Article] Isl1β Overexpression With Key β Cell Transcription Factors Enhances Glucose-Responsive Hepatic Insulin Production and Secretion2017

    • Author(s)
      Jung Yunshin、Zhou Ruyi、Kato Toshiki、Usui Jeffrey K、Muratani Masafumi、Oishi Hisashi、Heck Margarete M S、Takahashi Satoru
    • Journal Title

      Endocrinology

      Volume: 159 Issue: 2 Pages: 869-882

    • DOI

      10.1210/en.2017-00663

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Generation of CRISPR/Cas9-mediated bicistronic knock-in <i>ins1-cre</i> driver mice2016

    • Author(s)
      Yoshikazu Hasegawa, Yoshikazu Hoshino, Abdelaziz E. Ibrahim, Kanako Kato, Yoko Daitoku1, Yoko Tanimoto, Yoshihisa Ikeda, Hisashi Oishi, Satoru Takahashi, Atsushi Yoshiki, Ken-ichi Yagami, Hiroyoshi Iseki, Seiya Mizuno, Fumihiro Sugiyama
    • Journal Title

      Experimental Animals

      Volume: 65 Issue: 3 Pages: 319-327

    • DOI

      10.1538/expanim.16-0016

    • NAID

      130006882371

    • ISSN
      0007-5124, 1341-1357, 1881-7122
    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Remarks] 筑波大学 医学医療系 解剖学・発生学研究室

    • URL

      http://www.md.tsukuba.ac.jp/basic-med/anatomy/embryology/index.html

    • Related Report
      2017 Annual Research Report
  • [Remarks] 筑波大学 医学医療系 解剖学・発生学研究室ホームページ

    • URL

      http://www.md.tsukuba.ac.jp/basic-med/anatomy/embryology/index.html

    • Related Report
      2016 Research-status Report
  • [Remarks] 筑波大学 生命科学動物資源センター ホームページ

    • URL

      http://www.md.tsukuba.ac.jp/LabAnimalResCNT/

    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2019-03-29  

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